Browse > Article
http://dx.doi.org/10.3746/jkfn.2013.42.4.570

Effects of Turmeric (Curcuma longa L.) on Antioxidative Systems and Oxidative Damage in Rats Fed a High Fat and Cholesterol Diet  

Kim, Min-Sun (Dept. of Food Science, International University of Korea)
Chun, Sung-Sik (Dept. of Food Science, International University of Korea)
Choi, Jeong-Hwa (Dept. of Food and Nutrition, International University of Korea)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.42, no.4, 2013 , pp. 570-576 More about this Journal
Abstract
The purpose of this study was to investigate the effect of turmeric on antioxidative systems and oxidative damage in rats fed a high fat and cholesterol diet. A total 40 rats were divided into four experimental groups: a normal diet group (N), a high fat and cholesterol diet group (HF), a high fat and cholesterol diet group supplemented with 2.5% turmeric powder (TPA group) and a high fat and cholesterol diet group supplemented with 5% turmeric powder (TPB group). The serum glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activity of the turmeric supplemented groups were decreased compared to the HF group. The GPT activity of the TPB group was especially and significantly decreased compared to the HF group. Hepatic superoxide dismutase (SOD) of the TPB group was significantly increased compared to the HF group. However, there were no significant differences in the activities of hepatic glutathione peroxidase (GSHpx) and catalase (CAT) among all experimental groups. Hepatic glutathione S-transferase (GST) activity in the TPA and TPB groups were increased compared to the HF group. Hepatic superoxide radical content in mitochondria of the 5% turmeric supplemented group was significantly decreased compared to the HF group. Hepatic hydrogen peroxide content in the cytosol and mitochondria of the turmeric-supplemented groups were decreased compared to the HF group. Hepatic carbonyl values in the mitochondria of the turmeric supplemented groups were significantly decreased compared to the HF group. Thiobarbituric acid reaction substance (TBARS) values in the liver were significantly reduced in turmeric supplemented groups compared to the HF group. These result suggest that turmeric powder may reduce oxidative damage through the activation of antioxidative defense systems in rats fed high fat and cholesterol diets.
Keywords
turmeric; oxidative damage; antioxidative system; high cholesterol; free radical;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Oh HI, Park HB, Ju MS, Jung SY, Oh MS. 2010. Comparative study of anti-oxidant and anti-inflammatory activities between Curcumae longae Radix and Curcumae longae Rhizoma. Korean J Herbology 25: 83-91.   과학기술학회마을
2 Jacoby WB. 1978. The glutathione S-transferases: a group of multifunctional detoxification proteins. Adv Enzymol Relat Areas Mol Biol 46: 383-414.
3 Adaramoye OA, Nwosu IO, Farombi EO. 2012. Sub-acute effect of NG-nitro-I arginine methyl-ester (L-NAME) on biochemical indices in rats: Protective effects of Kolaviron and extract of Curcuma longa L. Pharmacognosy Res 4: 127-133.   DOI   ScienceOn
4 An BJ, Lee JY, Park TS, Pyeon JR, Bae HJ, Song MA, Baek EJ, Park JM, Con JH, Lee CE, Choi KI. 2006. Antioxidant activity and whitening effect of extraction conditions in Curcuma longa L. Korean J Medicinal Crop Sci 14: 168-172.   과학기술학회마을
5 Bus JS, Aust SD, Gibson JE. 1975. Lipid peroxidation: a possible mechanism for paraquat toxicity. Res Commun Chem Pathol Pharmacol 11: 31-38.
6 Kim KB, Yoo KH, Park HY, Jeong JM. 2006. Anti-oxidative activities of commercial edible plant extracts distributed in Korea. J Korean Soc Appl Biol Chem 49: 328-333.   과학기술학회마을
7 Plaa GL, Witscri H. 1976. Chemicals, drugs and lipid peroxidation. Annu Rev Pharmacol Toxicol 16: 125-141.   DOI   ScienceOn
8 Del Boccio GLD, Porreca EPA, Savini R, Feliciani P, Ricci G, Cuccurullo F. 1990. Aortic antioxidant defence mechanism time-related change in cholesterol-fed rabbits. Atherosclerosis 84: 127-135.
9 Mantha SV, Kalra J, Prasad K. 1996. Effects of probucol on hypercholesterolemia-induced changes in antioxidant enzymes. Life Sci 58: 503-509.   DOI   ScienceOn
10 Andrew MA, Matthew SM. 2000. Isolation of curcuma from tumeric. J Chem Educ 77: 359-362.   DOI   ScienceOn
11 An BJ, Lee JT, Park TS, Pyeon JR, Bae JH, Song MA, Beak EJ, Park JM, Son JH, Lee CE, Cho IK. 2006. Antioxidant activity and whitening effect of extraction condition in Curcuma longa L. Korean J Medicinal Crop Sci 14: 168-172.
12 Ammon HRT, Wahl MA. 1991. Pharmacology of Curcumin longa. Planta Med 57: 1-7.   DOI   ScienceOn
13 Fu Y, Zheng S, Lin J, Ryerse J, Chen A. 2008. Curcumin protects the rat liver from CCI4-caused injury and fibrogenesis by attenuating oxidative stress and suppressing inflammation. Mol Pharmacol 73: 339-409.
14 Jones DP, Eklow L, Orrenius S. 1981. Metabolism of hydrogen peroxide in isolated hepatocytes: relative contribution of catalase and glutathione peroxidase in decomposition of endogenously generated H2O2. Arch Biochem Biophys 210: 505-516.   DOI   ScienceOn
15 Liju VB, Jeena K, Kuttan R. 2011. An evaluation of antioxidant, anti-inflammatory, and antinociceptive activities of essential oil from Curcuma longa. L. Indian J Pharmacol 43: 526-531.   DOI   ScienceOn
16 Akira A, Kiyotake N, Teruo N. 1999. Antioxidative effects of turmeric, rosemary and capsicum extracts on membrane phospholipid peroxidation and liver lipid metabolism in mice. Biosci Biotechnol Biochem 63: 2118-2122.   DOI   ScienceOn
17 Ryn GY, No KH, Ryu SR, Yang HS. 2005. Study of separation and analysis method an effective component from UIGeum (Curcuma longa) and a contained curcumin as product of national and partial region cultures. Appl Chem 9: 57-60.
18 Kang WS, Kim SH, Park EJ, Yoon KR. 1998. Antioxidative property of turmeric (Curcumae Rhizoma) ethanol extract. Korean J Food Sci Technol 30: 266-271.   과학기술학회마을
19 Song EK, Cho H, Kim JS, Kim NY, An NH, Kim JA, Lee SH, Kim YC. 2001. Diarylheptanoids with free radical scavenging and hepatoprotective activity in vitro from Curcuma longa. Planta Med 67: 876-877.   DOI   ScienceOn
20 Masuda T, Isobe T, Jito A, Nakatani N. 1992. Antioxidative curcuminoids from rhizomes of Curcuma xanthorrhiza. J Phytochem 31: 3645-3649.   DOI   ScienceOn
21 Russell LR. 1988. High performance liquid chromatographic separation and spectral characterization of the pigments in tumeric and anatto. J Food Sci 53: 1823-1826.   DOI
22 Reitman S, Frankel S. 1957. A colorimetric method for the determination of serum blutamic oxaloacetic and glutamic pyruvic transaminase. Am J Clin Pathol 28: 56-63.   DOI
23 Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the antioxidation of pyrogallol and a cinvenient assay for superoxide dismutase. Eur J Biochem 47: 469-474.   DOI   ScienceOn
24 Lawrence RA, Burk RF. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71: 952-958.   DOI   ScienceOn
25 Azzi A, Montecucco C, Richter C. 1975. The use of acetylated ferricytochrome c for the detection of superoxide radicals produced in biological membrane. Biochem Biophys Res Commun 65: 597-603.   DOI   ScienceOn
26 Abei H, Wyss SR, Scherz B, Skvaril F. 1974. Heterogeneity of erythrocyte catalase II. Isolation and characterization of normal and variant erythrocyte catalase and their subunits. Eur J Biochem 48: 137-145.   DOI   ScienceOn
27 Habig WH, Pabst MJ, Jakoby WB. 1974. Glutathione Stransferase; the first enzymatic steps in mercapturic acid formation. J Biol Chem 249: 7130-7139
28 Gay C, Gebicki JM. 2000. A critical evaluation of the effect of sorbitol on the ferric-xylenol orange hydroperoxide assay. Anal Biochem 284: 217-220.   DOI   ScienceOn
29 Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn B, Shaliel S, Stadtment ER. 1990. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 186: 464-478.   DOI
30 Uchiyama M, Mihara M. 1978. Determination of malondialdehyde precursor in tissues by TBA test. Anal Biochem 86: 271-278.   DOI   ScienceOn
31 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275.
32 Sreel RGD, Torrie JH. 1990. Principles and procedures of statistics. Mcgrow Hill, New York, NY, USA.
33 Halliwell B, Gutteridge JMC. 1996. Free radicals, ageing, and disease. In Free Radicals in Biology and Medicine. Clarendon Press, London, UK. p 416.
34 Mantha SV, Kalra J, Prasad K. 1996. Effects of probucol on hyper cholesterolemia-induced changes in antioxidant enzymes. Life Sci 58: 503-509.   DOI   ScienceOn
35 Trackshel G, Maines MD. 1988. Characterization of glutathione S-transferase in rat kidney. Biochem J 252: 127-136.   DOI
36 Del Boccio G, Lapenna K, Porreca E, Pennelli A, Savini F, Feliciani P, Ricci G, Cuccurullo F. 1990. Aortic antioxidant defence mechanisms: time-related changes in cholesterolfed rabbits. Atheroscleosis 81: 127-135.   DOI
37 Lee JM, Choi SW, Cho SH, Rhee SJ. 2003. Effect of seeds extract of Paeonia lactiflora on antioxidative system and lipid peroxidation of liver in rats fed high-cholesterol diet. Korean J Nutr 36: 793-800.   과학기술학회마을
38 Wokozawa T, Nakagawa T, Kitani K. 2002. Antioxidative activity of green tea polyphenol in cholesterol-fet rats. J Agric Food Chem 50: 3549-3552.   DOI   ScienceOn
39 Jang JR, Hwang SY, Lim SY. 2010. Effects of extracts from dried yam on antioxidant and growth of human cancer cell lines. J Life Sci 20: 1365-1372.   과학기술학회마을   DOI   ScienceOn
40 Halliwell B, Gutteridge JMC. 1999. Antioxidant defenses. In Free Radicals in Biology and Medicine. 3rd ed. Oxford University Press, Oxford, UK. p 105-245.
41 Halliwell B, Aruoma OJ. 1991. DNA damage by oxygenderived species. FEBS Letters 281: 9-19.   DOI   ScienceOn
42 Lee JM. 1997. Protective effect of Ganoderma lucidum and Panax ginseng C.A. Meyer on oxidative damage. MS Thesis. Seoul National University, Seoul, Korea.
43 Oh H, Park H, Ju MS, Jung SY, Oh MS. 2010. Comparative study of anti-oxidant and anti-inflammatory activities between Curcumae longae Radix and Curcumae longae Rhizoma. Kor J Herbology 25: 83-91.   과학기술학회마을
44 Geoffrey NR, Amitahb C, Muraleedharan GN. 1998. Nobel bioactivities of Curcuma longa constituents. J Nat Prod 61: 542-545.   DOI   ScienceOn