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http://dx.doi.org/10.3746/jkfn.2013.42.3.355

The Effects of Soybean Protopectinase on Melanin Biosynthesis  

Yoo, Jin-Kyoun (Laboratory Affiliated with a CHA BIO F&C)
Lee, Jin-Hee (Laboratory Affiliated with a CHA BIO F&C)
Cho, Hyung-Yong (Laboratory Affiliated with a CHA BIO F&C)
Kim, Jung-Gook (Laboratory Affiliated with a CHA BIO F&C)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.42, no.3, 2013 , pp. 355-362 More about this Journal
Abstract
This study was performed to assess the antioxidant activities and whitening effects of protopectinase enzymes and mechanical maceration from soybeans on melanin synthesis. The whitening effects of enzyme treatment and mechanical maceration were examined by an in vitro mushroom tyrosinase assay and by assessing markers in B16BL6 melanoma cells. We assessed inhibitory effects on the expression of melanogenic enzymes, including tyrosinase, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) in B16BL6 cells. Inhibitory effects on free radical generation were determined by measuring DPPH and hydroxyl radical scavenging activities. In DPPH radical scavenging activity, enzyme treatment and mechanical maceration had a potent anti-oxidant activity in a dose-dependent manner and significantly inhibited tyrosinase activity in vitro and in B16BL6 melanoma cells. There was also an inhibition in the expression of tyrosinase, TRP-1, and TRP-2 in B16BL6 melanoma cells. Our results show that soybean protopectinase treatment inhibits melanogenesis, with the underlying mechanism possibly due to the inhibition of tyrosinase activity and tyrosinase, TRP-1, and TRP-2 expression. We suggest that soybean protopectinase should be contained as natural active ingredients for antioxidant and whitening cosmetics.
Keywords
enzyme; protopectinase; single cell plant; live cell; soybean;
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Times Cited By KSCI : 15  (Citation Analysis)
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