Browse > Article
http://dx.doi.org/10.3746/jkfn.2013.42.1.033

The Effect of Eisenia bicyclis Extracts on Bone Tissues in Ovariectomized Rats  

Park, Yong Soo (Dept. of Food and Nutrition, Silla University)
Kang, Min Suk (Dept. of Food and Nutrition, Silla University)
Kim, Bo Kyung (Dept. of Food and Nutrition, Silla University)
Kim, Mihyang (Dept. of Food and Nutrition, Silla University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.42, no.1, 2013 , pp. 33-39 More about this Journal
Abstract
Menopause is often associated with several chronic diseases, including osteoporosis, cardiovascular disease, and obesity. In this study, we investigated the ability of Eisenia bicyclis (EB) to prevent bone loss in ovariectomized rats, a model for postmenopausal osteoporosis. Extracts from EB obtained using ethanol or hot water were analyzed for total polyphenol content and osteoporosis effects in vivo. Total polyphenol content was higher with extraction by hot water compared to ethanol extraction. Fifty 8-week-old female Sprague-Dawley rats were randomly assigned to four groups: the group were sham-operated rats (SHAM), ovariectomized rats (OVX-CON), and ovariectomized rats that were treated with EB at 50 mg/kg body weight (OVX-EB50) and 200 mg/kg body weight (OVX-EB200), respectively. The diets were fed to rats for 6 weeks after their operation. We found that the alkaline phosphatase (ALP) activity was lower in the EB extract group compared to the OVX-CON group. Collagen and pyridinoline content, in bone and cartilage, were reduced by ovariectomy, but the supplemented EB extract groups exhibited higher concentrations in their bones. These results suggest that EB can be used for the industrial development of foods with therapeutic functions.
Keywords
Eisenia bicyclis; ALP; collagen; pyridinoline; ovariectomized rats;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Abe T, Chow JM, Lean JM, Chambers TJ. 1993. Estrogen does not restore bone lost after ovariectomy in the rat. J Bone Miner Res 8: 831-838.
2 Jeong SH, Lee HH, Han MY, Ryu CY, Yoon MC. 2002. Effects of fenofibrate on body weight and lipid metabolism in female mice. Journal of the Institute of Natural Science, Mokwon University 11: 27-32.
3 Gale SK, Sclafani A. 1977. Comparison of ovarian and hypothalamic obesity syndromes in the female rat: effect of diet palatability on food intake and body weight. J Comp Physiol Psychol 91: 381-392.   DOI
4 Lee SS, Park Yoon JH. 1989. Long-term effect of ovariectomy on body composition. Korean J Nutr 22: 102-107.   과학기술학회마을
5 Park WD, Hwang JS, Hur JW, Ahn SH, Park SK, Kwak CS. 1999. Activities of $\alpha$-D-mannosidase and $\beta$-D-mannosidase in patients with liver diseases. Korean J Gastroenterol 33: 211-221.
6 Riis BJ. 1993. Biochemical markers of bone turnover. II: Diagnosis prophylaxis, and treatment of osteoporosis. Am J Med 95: 17S-21S.   DOI   ScienceOn
7 Kim IG, Kim SB, Kim JG, Kim KC, Chun KC, Park HK, Lee KS. 1993. Serum enzymes as indicators of radiation exposure in rat. J Radiation Protection 18(2): 37-44.   과학기술학회마을
8 Mcconkey B, Fraser GM, Bligh AS, Whiteley H. 1963. Transparent skin and osteoporosis. Lancet 281: 693-695.   DOI   ScienceOn
9 Haroun-Bouhedja F, Ellouali M, Sinquin C, Boisson-Vidal C. 2000. Relationship between sulfate groups and biological activities of fucans. Thromb Res 100: 453-459.   DOI   ScienceOn
10 Colliec S, Fischer AM, Tapon-Bretaudiere J, Boisson C, Durand P, Jozefonvicz J. 1991. Anticoagulant properties of a fucoidan fraction. Thromb Res 64: 143-154.   DOI   ScienceOn
11 Kang JW. 1968. Marine algae. In I llustrated encyclopedia of fauna & flora of Korea. Ministry of Education. Vol 8, p 148-151.
12 Jang YH, Choi SW, Cho SH. 2008. Effect of Eisenia bicyclis and its pill on serum status in rats fed high fat diet. Korean J Nutr 41: 5-12.   과학기술학회마을
13 Kim YM, Han CK, Bang SJ, Park JH. 2006. Effects of laminaran from Eisenia bicyclis on serum lipids in rats fed high cholesterol diet. J Korean Soc Food Sci Nutr 35: 841-846.   과학기술학회마을   DOI   ScienceOn
14 Cahyana AH, Shoto Y, Kinoshita Y. 1992. Pyropheophytin a as an antioxidative substance from the marine alga, Arame (Eisenia bicyclis). Biosci Biotech Biochem 56: 1533-1535.   DOI
15 AOAC. 2005. Official Method of Analysis. 18th ed. Association of Official Analytical Chemists, Washington, DC, USA. Vol 45, p 21-22.
16 Woessner JF Jr. 1961. The determination of hydroxyproline in tissue and protein sample containing small proportion of this imino acid. Arch Biochem Biophys 93: 440-447.   DOI   ScienceOn
17 Arakawa N, Kim M, Otsuka M. 1992. An improved highperformance liquid chromatographic assay for the determination of pyridinoline in connective tissues. J Nutr Sci Vitaminol (Tokyo) 38: 375-380.
18 Kim MH, Otsuka M, Arakawa N. 1994. Age-related changes in the pyridinoline content of guinea pigs cartilage and achilles tendon collagen. J Nutr Sci Vitaminol (Tokyo) 40:95-103.   DOI   ScienceOn
19 Lee S, Jang MK, Kim NY, Jang HJ, Lee DG, Kim M, Kim YY, Kim SG, Yoo BH, Lee SH. 2010. Verification of the fractions with strong estrogenic activities from brown algae. J Life Sci 20: 1807-1811.   과학기술학회마을   DOI   ScienceOn
20 Wronski TJ, Cintron M, Dann LM. 1988. Temporal relationship between bone loss and increased bone turnover in ovariectomized rats. Calcif Tissue Int 43: 179-183.   DOI   ScienceOn
21 Kuboki Y, Mechanic GL. 1982. Comparative molecular distribution of cross-links in bone and dentin collagen. Structure- function relationships. Calcif Tissue Int 34: 306-308.   DOI   ScienceOn
22 Robins SP, Bailey AJ. 1977. The chemistry of the collagen cross-links. Characterization of the products of reduction of skin, tendon and bone with sodium cyanoborohydride. Biochem J 163: 339-346.   DOI
23 Seigel RC. 1976. Collagen cross-linking. Synthesis of collagen cross-links in vitro with highly purified lysyl oxidase. J Biol Chem 251: 5786-5792.
24 Noda M, Roodon GA. 1989. Transcriptional regulation of osteopontin production in rat osteoblast-like cells by parathyroid hormone. J Cell Biol 108: 713-718.   DOI   ScienceOn
25 Ninomiya Y, Showater AM, Olsen BR. 1984. The role of extracellular matrix in development. Alan R Liss Inc., New York, NY, USA. p 225.
26 Schiele F, Henny J, Hitz J, Petitclerc C, Gueguen R, Siest G. 1983. Total bone and liver alkaline phosphatases in plasma: biological variations and reference limits. Clin Chem 29: 634-641.
27 Raisz LG. 1988. Local and systemic factors in the pathogenisis of osteoporosis. N Engl J Med 318: 818-828.   DOI   ScienceOn
28 Hang D, Choi HS, Kang SC, Kim KR, Sohn ES, Kim MH, Pyo S, Son E. 2005. Effects of fucoidan on NO production and phagocytosis of macrophages and the proliferation of neuron cells. J Food Sci Nutr 10: 344-348.   과학기술학회마을   DOI   ScienceOn
29 Sung JH, Ha S, Im MH, Im JG, Kang KS. 2002. Seaweed and health. In Foods and Health. Hyungseol Press Co., Seoul, Korea. p 190.
30 So MJ, Kim BK, Choi MJ, Kim KY, Rhee SH, Cho EJ. 2007. Protective activity of fucoidan and alginic acid against free radical-induced oxidative stress under in vitro and cellular system. J Food Sci Nutr 12: 191-196.   과학기술학회마을   DOI   ScienceOn
31 Johnston CC Jr, Hui SI, Witt RM, Appledorn R, Baker RS, Longcope C. 1985. Easex steroids. J Clin Endocrinol Metab 61: 905-911.   DOI
32 Richelson LS, Wahner HW, Melton LJ 3rd, Riggs BL. 1984. Relative contributions of aging and estrogen deficiency to postmenopausal bone loss. N Engl J Med 311: 1273-1275.   DOI   ScienceOn
33 Smith DM, Nance WE, Kang KW, Chrustian JC, Johnstone CC Jr. 1973. Genetic factors in determining bone mass. J Clin Invest 52: 2800-2808.   DOI   ScienceOn
34 Kim SY, Chang SY, Oh HJ. 1997. Comparison of bone mineral density and lipid profiles in pre and postmenopausal women. J Korean Acad Fam Med 18: 910-917.
35 Kimble RB, Vannice JL, Bloedowl DC, Thompson RC, Hopfer W, Kung VT, Brownfield C, Pacifici R. 1994. Interleukin-1 receptor antagonist decreases bone loss and bone resorption in ovariectomized rats. J Clin Invest 93: 1959-1967.   DOI   ScienceOn
36 Dempster DW, Birchman R, Xu R, Lindsay R, Shen V. 1995. Temporal changes in cancellous bone structure of rats immediately after ovariectomy. Bone 16: 157-161.   DOI   ScienceOn
37 Tiki ML, Allison GT, Naik K, Karry SK. 2003. Malondialdehyde oxidation of cartilage collagen by chondrocytes. Osteoarthritis Cartilage 11: 159-166.   DOI   ScienceOn
38 Fujimoto D, Hirama M, Iwa T. 1982. Histidinoalanine, a new crosslinking amino acid, in calcified tissue collagen. Biochem Biophys Res Commun 104: 1102-1106.   DOI   ScienceOn
39 Odell WD, Heath H 3rd. 1993. Osteoporosis pathophysiology, prevention, diagnosis, and treatment. Dis Mon 39:789-867.
40 Clark AP, Schuttinga JA. 1992. Targeted estrogen/progesterone replacement therapy for osteoporosis: calculation of health care cost savings. Osteoporos Int 2: 195-200.   DOI   ScienceOn
41 Holland EF, Studd JW, Mansell JP, Leather AT, Bailey AJ. 1994. Changes in collagen composition and cross-links in bone and skin of osteoporotic postmenopausal women treated with percutaneous estradiol implants. Obstet Gynecol 83:180-183.