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http://dx.doi.org/10.3746/jkfn.2013.42.12.1949

Determination of β-Carotene and Retinol in Korean Noodles and Bread Products  

Shin, Jung-Ah (Dept. of Food Science and Technology, Chungnam National University)
Chun, Ji Yeon (Dept. of Food Engineering, Sunchon National University)
Lee, Junsoo (Dept. of Food Science and Technology, Chungbuk National University)
Shin, Ki Yong (Nutrition Safety Policy Division, Ministry of Food and Drug Safety)
Lee, Soon Kyu (Nutrition Safety Policy Division, Ministry of Food and Drug Safety)
Lee, Ki-Teak (Dept. of Food Science and Technology, Chungnam National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.42, no.12, 2013 , pp. 1949-1957 More about this Journal
Abstract
The contents of ${\beta}$-carotene and retinol in processed and restaurant foods, such as Korean noodles, mandus, rice cakes and bread products, were quantified by high-performance liquid chromatography (HPLC) with UV/visible and fluorescence detector, respectively. Samples were collected from different local areas (i.e. Gangwon-do, Gyeonggi-do, Gyeongsang-do, Seoul, Jeolla-do, and Chungcheong-do). After homogenization, samples were hydrolyzed by direct alkali saponification; thereafter, fat-soluble components were extracted by a mixture of n-hexane/ethylacetate (85:15, v/v), containing 0.01% butylated hydroxytoluene (BHT). ${\beta}$-carotene and retinol contents in infant formula used as an in-house material for the analytical quality control. Among 14 Korean noodles, high contents of ${\beta}$-carotene were found in Bibim-Guksu (average 442.43 ${\mu}g/100g$) and Jjolmyeon (average 301.39 ${\mu}g/100g$). In 4 Korean mandus, the highest contents of ${\beta}$-carotene was determined in Kimchi-mandu (average 197.64 ${\mu}g/100g$), resulting in 33.3 RE of the converted vitamin A. Among 12 Korean rice cakes, Maeun-Tteokbokki and Modm-Chaltteok contained relatively high content of ${\beta}$-carotene with 205.11 and 41.33 ${\mu}g/100g$, respectively, while retinol was detected only in Maeun- Tteokbokki (1.65~10.45 ${\mu}g/100g$). In addition, among 8 bread products, 77.3 RE of pastry, 51.2 RE of buttercream- bread, and 41.4 RE of morning roll were found as the contents of the converted vitamin A.
Keywords
${\beta}$-carotene; retinol; retinol equivalent; direct saponification; processed and restaurant foods;
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