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http://dx.doi.org/10.3746/jkfn.2013.42.12.1899

Cholesterol-lowering Effects of Unripe Black Raspberry Water Extract  

Choi, Hye Ran (Gochang Black Raspberry Research Institute)
Lee, Su Jung (Gochang Black Raspberry Research Institute)
Lee, Jung-Hyun (Gochang Black Raspberry Research Institute)
Kwon, Ji Wung (Gochang Black Raspberry Research Institute)
Lee, Hee Kwon (Gochang Black Raspberry Research Institute)
Jeong, Jong Tae (Gochang Black Raspberry Research Institute)
Lee, Tae-Bum (Gochang Black Raspberry Research Institute)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.42, no.12, 2013 , pp. 1899-1907 More about this Journal
Abstract
We investigated the effects of unripe black raspberry water extract (UBR-W) and oxidation-LDL treatment on cholesterol levels. Experiments using an established human hepatocellular carcinoma cell line (HepG2) showed a time-dependent increase in expression of LDL receptor after UBR-W treatment. Expression of LDL receptor-related genes, such as SREBP1 and 2, increased upon UBR-W treatment. However, expression of HDL-related genes was unaffected by UBR-W. HMG-CoA reductase activity was reduced by UBR-W treatment, whereas HMG-CoA mRNA expression significantly increased. In addition, the ApoB/ApoA1 mRNA level, which is a predictor of cardiovascular risk, was reduced in a time-dependent manner by UBR-W treatment. Macrophage-like cells (RAW 264.7) showed increased expression of ox-LDL-related genes, such as CD36, scavenger receptor-A, adipophilin, and PPAR-gamma, upon ox-LDL treatment compared to untreated control cells, and quantitative lipid analysis indicated a dramatic increase in lipid accumulation. However, UBR-W treatment significantly reduced expression of ox-LDL-related genes and largely prevented lipid accumulation. The results indicate that UBR-W mediates a cholesterol-lowering effect via inhibition of cholesterol synthesis and induction of LDL uptake through SREBP.
Keywords
unripe black raspberry; cholesterol; LDL receptor; HMG-CoA reductase; ox-LDL;
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