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http://dx.doi.org/10.3746/jkfn.2012.41.7.901

Ishige sinicola Extracts Induce Apoptosis via Activation of a Caspase Cascade in Human HeLa Cells  

Cho, Byoung-Ok (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Ryu, Hyung-Won (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
So, Yang-Kang (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Jin, Chang-Hyun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Byun, Myung-Woo (Dept. of Culinary Nutrition, Woosong University)
Kim, Wang-Geun (Rare Isotope Science Project Team, Institute for Basic Science)
Jeong, Il-Yun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.41, no.7, 2012 , pp. 901-906 More about this Journal
Abstract
The purpose of this study was to elucidate the anti-proliferative effect and the mechanisms underlying apoptosis induced by a methanol extracts from Ishige sinicola (ISE) in HeLa cells. ISE treatment for 24 hr significantly inhibited cell viability in a dose-dependent manner. Apoptosis was detected by Hoechst 33258 staining and an annexin V/PI assay after 24 hr treatment. Moreover, ISE treatment triggered the cleavage of caspase-8, -9, -3, and poly(ADP-ribose) polymerase (PARP) in dose-dependent and time-dependent manners. In addition, z-VAD-fmk, a general caspase inhibitor, blocked ISE-induced cell death. Taken together, these results suggest that ISE-induced apoptosis is mediated by the activation of a caspase cascade in HeLa cells.
Keywords
Ishige sinicola; apoptosis; caspase; poly(ADP-ribose) polymerase (PARP);
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Times Cited By KSCI : 5  (Citation Analysis)
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