Browse > Article
http://dx.doi.org/10.3746/jkfn.2012.41.7.895

Effects of Fractions from Benincasa hispida on Inhibition of Adipogenesis in 3T3-L1 Preadipocytes  

You, Yang-Hee (Dept. Food and Nutrition, and Human Ecology Research Institute, Chonnam National University)
Jun, Woo-Jin (Dept. Food and Nutrition, and Human Ecology Research Institute, Chonnam National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.41, no.7, 2012 , pp. 895-900 More about this Journal
Abstract
The effects of three fractions, hexane (BHHH), chloroform (BHHC), and ethyl acetate (BHHE), from water extract of Benincasa hispida on the underlying mechanisms of adipogenesis were investigated in 3T3-L1 cells. Intracellular lipid droplets were stained with Oil Red O dye and quantified. Compared to control, lipid accumulation significantly decreased by 11% and 13% upon treatment with BHHC and BHHE, respectively at a concentration of 50 ${\mu}g/mL$. Intracellular triglyceride (TG) levels were also reduced by 21% and 16%, respectively, at the same concentration. To determine the mechanism behind the reductions in TG content and lipid accumulation, glycerol release and expression levels of adipogenic marker genes were measured. The levels of free glycerol released into culture medium increased by 13% and 17% upon treatment with BHHC and BHHE, respectively. In subsequent measurements using real-time polymerization chain reaction, the mRNA levels of $PPAR{\gamma}$, C/$EBP{\alpha}$, and leptin significantly decreased upon treatment with BHHE (45%, 67%, and 35%) in comparison with non-treated control. These results suggest that BHHE inhibits adipocyte differentiation by blocking $PPAR{\gamma}$, C/$EBP{\alpha}$, and leptin gene expression in 3T3-L1 cells, resulting in reduced lipid accumulation, increased glycerol release, and intracellular triglycerides.
Keywords
Benincasa hispida; adipogenesis; 3T3-L1; lipid accumulation; triglyceride;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Rosen ED, Hsu CH, Wang X, Sakai X, Freeman MW, Gonzalez FJ, Spiegelman BM. 2001. C/EBP$\alpha$ induces adipogenesis through PPAR: a unified pathway. Genes Dev 16: 22-26.
2 Morrison RF, Farmer SR. 2000. Hormonal signaling and transcriptional control of adipocyte differentiation. J Nutr 130: 3116-3121.   DOI
3 Rosen ED, Macdougald OA. 2006. Adipocyte differentiation from the in side out. Nat Rev Mol Cell Biol 7: 885-896.   DOI
4 Lim SJ, Jeong JG, Kim MW, Choi SS, Han HK, Park JE. 2003. Effects of Benincasa hispida intake blood glucose and lipid level in streptozotocin induced diabetic rats. Kor J Nutr 36: 335-343.
5 Huh J. 1994. The handbook of oriental medicine. Namsandong, Daegu, Korea. p 1170.
6 Warier PK. 1994. Indian medicinal plants. Orient longman limited, Delhi, India. p 261.
7 Sharma LK. 1984. Food medicines. Practical nature cure. Natural Care Publishing House, Pudukkottai, India. p 169.
8 Lee JM, Yoon HK, Lee YH, Park JJ, You YH, Jang JY, Yang JW, Jun WJ. 2010. The potential effects of ethyl acetate fraction from Curcuma longa L. on lipolysis in differentiated 3T3-L1 adipocytes. J Med Food 13:1-7.   과학기술학회마을   DOI
9 Jeong HJ, Yoon SJ, Pyun YR. 2008. Polysaccharides from edile mushroom Hinmogi (Tremella fuciformis) inhibit differentiation of 3T3-L1 adipocyte reducing mRNA expression of PPAR$\gamma$, CEPB$\alpha$, and leptin. Food Sci Biotechnol 17: 267-273.
10 Liu F, Kim J, Li Y, Liu X, Li J, Chen X. 2001. An extract of Lagerstroemia speciosa L has insulin-like glucose uptake stimulatory and adipocyte differentiation-inhibitory activities in 3T3-L1 cells. J Nutr 131: 2242-2247.   DOI
11 Spiegelman BM, Flier S. 1996. Adipogenesis and obesity; rounding out the big picture. Cell 87: 377-389.   DOI   ScienceOn
12 Kopelman PG. 2000. Obesity as a medical problem. Nature 404: 635-643.   DOI
13 Visscher TL. 2001. The public health impact of obesity. Annu Rev Publ Health 22: 355-375.   DOI   ScienceOn
14 Cha SY, Jang JY, Lee YH, Lee GY, Lee HJ, Hwang KT, Kim YJ, Jun YJ, Lee JM. 2010. Lipolytic effect of methanol extracts from Luffa cylindrica in mature 3T3-L1 adipocytes. J Korean Soc Food Sci Nutr 39: 813-819.   과학기술학회마을   DOI
15 Frayn KN, Karpe F, Fielding BA, Macdonald IA, Coppack SW. 2003. Integrative physiology of human adipose tissue. Int J Obes Relat Metab Disord 27: 875-888.   DOI   ScienceOn
16 Rosen ED, Walkey CJ, Ouigserver P, Spiegelman BM. 2000. Transcriptional regulation of adipogenesis. Genes & Dev 14: 1293-1307.
17 Jeon T, Hwang SG, Hirai S, Matsui T, Yano H, Kawda T, Lim BU, Ki D. 2004. Red yeast rice extract suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells. Life Sci 75: 31945-3203.
18 Cowherd RM, Lyle RE, McGehee Jr RE. 1999. Molecular regulation of adipocyte differentiation. Semin Cell Dev Biol 10: 3-10.   DOI   ScienceOn
19 Cornelius P, Mac Dougald OA, Lane MD. 1994. Regulation of adipocyte development. Annu Rev Nutr 14: 99-129.   DOI   ScienceOn
20 Gustafson B, Jack MM, Cushman SW, Smith U. 2005. Adiponectin gene activation by thiazolidinediones requires PPAR gamma 2, but not CEBP alpha-evidence for differential regulation of the aP2 and adiponectin genes. Biochem Biophys Res Commun 308: 933-939.
21 Hsu CL, Yen GC. 2007. Effects of flavonoids and phenolic acids on the inhibition of adipogenesis in 3T3-L1 adipocyte. J Agric Food Chem 55: 8404-8410.   DOI   ScienceOn
22 Sato M, Hiragun A, Mitsui H. 1980. Preadipocytes possess cellular retinoid binding proteins and their differentiation is inhibited by retinoids. Biochem Biophys Res Commun 95: 1839-1845.   DOI
23 Aihaud G, Grimaldu P, Negrel R. 1992. Cellular and molecular aspects of adipose tissue development. Annu Rev Nutr 12: 207-233.   DOI   ScienceOn
24 Boney CM, Staats BM, Atiles AD, Dercole AJ. 1994. Expression on insulin-like growth factor-I(IGF-I) and IGF-binding proteins during adipogenesis. Endocrinology 135: 1863-1868.   DOI   ScienceOn
25 Choi JH, Rhim CH, Kim JY, Yang JS, Choi JS, Byun DS. 1986. Basic studies on the development of diet for the treatment of obesity in the inhibitory effect of alginic acid as a diet fiber on obesity. J Kor Fish Soc 19: 303-311.   과학기술학회마을
26 Kawada T, Aoki N, Kamei Y, Maeshige K, Nishiu S, Sugimoto E. 1990. Comparative investigation of vitamins and their analogues on terminal differentiation from preadipocytes to adipocytes of 3T3-L1 cells. Comp Biochem Physiol 96: 323-326.   DOI   ScienceOn
27 Cno M, Aratani Y, Kitagawa I, Kitagawa Y. 1990. Ascorbic acid phosphate stimulate type IV collagen synthesis and accelerate adipose conversion of 3T3-L1 cells. Exp Cell Res 187: 309-314.   DOI   ScienceOn
28 Harrison SA, Buxtaon JM, Clancy BM, Czech MP. 1991. Evidence that erythroid-type glucose transporter intrinsic activity is modulated by cadmium treatment of mouse 3T3-L1 cells. J Biol Chem 266: 19438-19449.
29 Darlington GJ, Ross SE, MacDougald OA. 1998. The role of C/EBP genes in adipocyte differentiation. J Biol Chem 273: 30057-30060.   DOI