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http://dx.doi.org/10.3746/jkfn.2012.41.7.881

In Vitro Profiling of Bacterial Influence and Herbal Applications of Lonicerae Flos on the Permeability of Intestinal Epithelial Cells  

Lee, Sin-Ji (Dept. of Oriental Rehabilitation Medicine, Dongguk University)
Lee, Myeong-Jong (Dept. of Oriental Rehabilitation Medicine, Dongguk University)
Jung, Ji-Eun (Dept. of Oriental Rehabilitation Medicine, Dongguk University)
Kim, Ho-Jun (Dept. of Oriental Rehabilitation Medicine, Dongguk University)
Bose, Shambhunath (Dept. of Oriental Rehabilitation Medicine, Dongguk University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.41, no.7, 2012 , pp. 881-887 More about this Journal
Abstract
This study was designed to examine influence of bacteria and herbal applications of Lonicerae Flos on the permeability of intestinal epithelial cells. The HCT-116 human intestinal cell was used as an in vivo model of "leaky gut". Dextran sodium sulphate (DSS) was used to induce an increase in the permeability of epithelial cell tight junctions. Probiotics including Lactobacillus acidophilus, Lactobacillus plantarum, Salomonella spp. and Staphylococcus aureus were used to evaluate bacterial influence on the intestinal epithelial cells. Herbal extract Lonicerae Flos was also assessed to find out what kind of effects it has when used alone or fermented with Lactobacillus plantarum. The permeability of intestinal cell lines was assessed using transepithelial electrical resistance (TEER) and horseradish peroxide (HRP) assessments. This data provides evidence for the probiotic benefits of intestinal permeability. The data also provides promising evidence of herbal effects on reducing intestinal permeability, especially when fermented with probiotics.
Keywords
permeability of intestinal epithelial cells; tight junction; leaky gut syndrome; probiotics; herbal fermentation;
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Times Cited By KSCI : 5  (Citation Analysis)
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