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http://dx.doi.org/10.3746/jkfn.2012.41.5.655

Analyses of Pungency-Related Factors of Field and Rice Paddy Garlic  

Oh, Hye-Lim (Dept. of Food & Nutrition, Chungnam National University)
Kim, Na-Yeon (Dept. of Food & Nutrition, Chungnam National University)
Sohn, Chan-Wok (Dept. of Food & Nutrition, Chungnam National University)
Ryu, Bo-Ram (Dept. of Food & Nutrition, Chungnam National University)
Yoon, Jun-Hwa (Dept. of Food & Nutrition, Chungnam National University)
Kim, Mee-Ree (Dept. of Food & Nutrition, Chungnam National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.41, no.5, 2012 , pp. 655-660 More about this Journal
Abstract
The purpose of this study is to evaluate pungency-related factors of field garlic (FG) and rice paddy garlic (RG) from Youncheon province. Allicin, alliin, and S-allyl-L-cysteine (SAC) contents were analyzed by HPLC. In addition, activities of alliinase, GTPase (${\gamma}$-glutamyltranspeptidase), and pyruvate content of garlic were measured. The moisture content of RG (65.86%) was higher than that of FG (63.34%). However, crude lipid, crude protein, crude ash, and carbohydrate contents of RG were lower than those of FG. The alliin contents of FG and RG were 8.97 and 8.22 mg/g, respectively. The allicin content of FG (2.83 mg/g) was higher than that of RG (2.22 mg/g). Further, SAC content of FG (1.74 mg/g) was higher than that of RG (0.104 mg/g). Alliinase activities of FG and RG were similar, whereas the GTPase activity of FG was higher than that of RG. These results show that the stronger pungency of FG is due to the higher amount of alliin and SAC as well as the higher activity of GTPase compared to RG.
Keywords
garlic; alliin; allicin; S-allylcysteine; HPLC;
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  • Reference
1 Kim MR, Ahn SY. 1983. Garlic flavor. J Korean Soc Food Sci Nutr 12: 176-187.
2 Koch HP, Lawson LD. 1996. Garlic: the science and therapeutic application of Allium sativum L. and related species. Williams & Wilkins, Baltimore, MD, USA. p 37-209.
3 Amagase H, Petesch BL, Matsuura H, Kasuga S, Itakura Y. 2001. Intake of garlic and its bioactive components. J Nutr 131: 955S-962S.   DOI
4 Cavallito CJ, Bailey JH. 1944. Allicin, the antibacterial principle of Allium sativum. I. Isolation, physical properties and antibacterial action. J Am Chem Soc 66: 1950-1951.   DOI
5 Stoll A, Seebeck E. 1951. Chemical investigations on alliin the specific principle of garlic. Adv Enzymol 11: 377-379.
6 Jones MG, Hughes J, Tregova A, Milne J, Tomsett AB, Collin HA. 2004. Biosynthesis of the flavour precursors of onion and garlic. J Exp Bot 55: 1903-1918.   DOI   ScienceOn
7 Amagase H. 2006. Clarifying the real bioactive constituents of garlic. J Nutr 136: 716S-725S.   DOI
8 Kim MR, Mo EK. 1995. Volatile sulfur compounds in pickled garlic. Korean J Food Sci Technol 11: 133-139.
9 Kim MR, Yoon JH, Sok DE. 1994. Correlation between pungency and allicin contents in pickled garlic during aging. J Korea Soc Food Sci Nutr 23: 805-810.
10 Kim MR, Song MJ, Jhee OK, Ahn SY. 1994. Purification and characterization of alliinase from garlic of Korean origin. Korean J Food Sci Technol 10: 376-380.
11 Shin JH, Lee SJ, Jung WJ, Kang MJ, Sung NJ. 2011. Physicochemical characteristics of garlic (Allium sativum L.) on collected from the different regions. J Agric Life Sci 45: 103-114.
12 Nam SS, Chio IH, Chio HS, Kim CW, Bae SK, Bang JK. 2005. Characteristics of new variety 'Saengol' of southern type garlic. Korean J Hort Sci Technol 12: 409-412.
13 Kim CB, Kim CY, Park M, Lee DH, Choi J. 2000. Effect of chemical properties of cultivation soils on the plant growth and quality of garlic. Korean J Soil Sci Fert 33: 333-339.
14 Hwang KS, Lee SJ, Kwack YH, Kim KS. 1997. Soil chemical properties of major vegetable producing open fields. Korean J Soil Sci Fert 30: 146-151.
15 Lee YK, Sin HM, Woo KS, Hwang IG, Kang TS, Jeong HS. 2008. The relationship between functional quality of garlic and soil composition. Korean J Food Sci Technol 40: 31-35.
16 AOAC. 1995. Official methods of analysis. 15th ed. Association of Official Analytical Chemists, Arlington, VA, USA.
17 Arnault I, Christidès JP, Mandon N, Haffner T, Kahane R, Auger J. 2003. High-performance ion-pair chromatography method for simultaneous analysis of alliin, deoxyalliin, allicin and dipeptide precursors in garlic products using multiple mass spectrometry and UV detection. J Chromatogr 991: 69-75.   DOI   ScienceOn
18 Kim MR. 1981. Changes in pungency during garlic pickle. MS Thesis. Seoul National University, Seoul, Korea.
19 Lancaster JE, Shaw ML. 1991. Metabolism of $\gamma$-glutamyl peptides during development, storage and sprouting of onion bulbs. Phytochemistry 30: 2857- 2859.   DOI
20 Yang CH, Yoo CH, Shin BW, Kim JD, Kang SW. 2006. Effect of band spotty fertilization on yields and nutrient utilization of garlic (Allium sativum L.) in plastic film mulching cultivation. Korean J Soil Sci Fert 39: 380-385.
21 Jeong JH. 1998. Quality changes of fresh garlic paste during storage. J Korean Soc Food Sci Nutr 11: 279-282.
22 Park HH, Lee YN, Lee KH, Kim TH. 2004. World of garlic. Hyoil books, Seoul, Korea. p 91-94.
23 Chung DH, Jeong SW. 2005. Garlic science. Worldscience, Seoul, Korea. p 63.
24 Ohsumi C, HAyashi T. 2004. The oligosaccharide unit of the xyloglucans in the cell walls of bulbs of onion, garlic and their hybrid. Plant Cell Physiol 35: 963-967.
25 Wi SU. 2003. Isolation of alliin in garlic and its quantitative determination by high performance liquid chromatography and studies of the antimicrobial effects of alliin and ethanol extracts from Korean garlic (Allium sativum L.). J Korean Soc Food Sci Nutr 16: 296-302.
26 Ziegler SJ, Sticher O. 1989. HPLC of S-alk(en)yl-L-cysteine derivatives in garlic including quantitative determination of (+)-S-allyl-L-cysteine sulfoxide (Alliin). Planta Med 55: 372-378.   DOI
27 Shin DS. 2001. Effect of food components and processing condition on antimicrobial of garlic-alliinase reaction compounds. MS Thesis. Chung-Ang University, Seoul, Korea.
28 Tomofumi M, Asako H, Mitsuyo S, Mami Y, Kazuki S. 1998. Alliinase [S-alk(en)yl-L-cystein suloxide lyase] from Allium tuberosum (Chinese chive). Eur J Biochem 257: 21-30.   DOI   ScienceOn
29 Choi YH, Shim YS, Kim CT, Lee C, Shin DB. 2007. Characteristics of thiosulfinates and volatile sulfur compounds from blanched garlic reacted with alliinase. Korean J Food Sci Technol 39: 600-607.