Browse > Article
http://dx.doi.org/10.3746/jkfn.2012.41.4.456

Anti-Inflammatory Activity of Austroinulin from Stevia rebaudiana in LPS-induced RAW264.7 Cells  

Byun, Myung-Woo (Dept. of Culinary Netrition, Woosong University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.41, no.4, 2012 , pp. 456-461 More about this Journal
Abstract
The leaves of $Stevia$ $rebaudiana$ are well-known in Japan, Korea, and China as a natural sweetener. Medicinal uses of this plant originated in Paraguay and Brazil in the form of aqueous decoctions of the leaves used as a contraceptive agent and for the treatment of hyperglycemia. In the present study, the antioxidant, anti-hypertension, and anti-inflammatory activities of $S.$ $rebaudiana$ extracts are investigated for their use in food. The biologically-active compound was isolated and purified from $S.$ $rebaudiana$. The isolated compound was identified as austroinulin ($C_{20}H_{34}O_3$; molecular weight 322) by mass, IR spectrophotometry, 1D, and 2D-NMR. Austroinulin was characterized as a diterpenoid possessing a 3-methylpenta-2,4-dienyl at C-9. When subjected to an inflammatory mediator inhibitory assay from lipopolysaccharide (LPS)-activated macrophages, the austroinulin inhibited the enhanced production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) expression (10 ${\mu}g$/mL＝67.9 and 45.1%, respectively). This was significant and dose-dependent. The results suggest that austroinulin from $S.$ $rebaudiana$ inhibited the NO and iNOS in RAW 264.7 cells.
Keywords
$Stevia$ $rebaudiana$; austroinulin; RAW264.7; anti-inflammation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Zhao F, Wang L, Liu K. 2009. In vitro anti-inflammatory effects of arctigenin, a lignan from Arctium lappa L., through inhibition on iNOS pathway. J Ethnopharmacol 122: 457-462.   DOI   ScienceOn
2 Metzger BT, Barnes DM, Reed JD. 2008. Purple carrot (Daucus carota L.) polyacetylenes decrease lipopolysaccharideinduced expression of inflammatory proteins in macrophage and endothelial cells. J Agric Food Chem 56: 3554-3560.   DOI   ScienceOn
3 Rimon G, Sidhu R, Lauver D, Lee J, Sharma N, Yuan C, Frieler R, Trievel R, Lucchesi B, Smith W. 2010. Coxibs interfere with the action of aspirin by binding tightly to one monomer of cyclooxygenase-1. PNAS 107: 28-33.   DOI   ScienceOn
4 Anderson GD, Keys KL, Ciechi PAD, Masferrer JL. 2009. Combination therapies that inhibit cyclooxygenase-2 and leukotriene synthesis prevent disease in murine collagen induced arthritis. Inflamm Res 58: 109-117.   DOI   ScienceOn
5 Dogne JM, Hanson J, Supuran C, Pratico D. 2006. Coxibs and cardiovascular side-effects: from light to shadow. Curr Pharm Des 12: 917-975.
6 Makins R, Ballinger A. 2003. Gastrointestinal side effects of drugs. Expert Opin Drug Saf 2: 421-429.   DOI
7 Lee MK, Choi YJ, Sung SH, Shin DI, Kim JW, Kim YC. 1995．Antihepatotoxic activity of icariin, a major constituent of Epimedium koreanum. Plant Med 61: 523-526.   DOI   ScienceOn
8 Quijano L, Calderon JS, Comez F, Vega JL, Rios T. 1982. Diterpenes from Stevia monardaefolia. Phytochemistry 21: 1369-1371.   DOI   ScienceOn
9 Sholichin M, Yamasaki K, Miyama R, Yahara S, Tanaka O. 1980. Labdane-type diterpenes from Stevia rebaudiana. Phytochemistry 19: 326-327.   DOI   ScienceOn
10 Lee ES, Ju HK, Moon TC, Lee E, Jang Y, Lee SH, Son JK, Baek SH, Chang HW. 2004. Inhibition of nitric oxide and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) production by propenone compound through blockade of nuclear factor (NF)-kB activation in cultured murine macrophage. Biol Pharm Bull 27: 617-620.   DOI   ScienceOn
11 Park EK, Shin YW, Lee HU, Kim SS, Lee YC, Lee BY, Kim DH. 2005. Inhibitory effect of ginsenoside Rb1 and compound K on NO and prostaglandin E2 biosyntheses of RAW264.7 cells induced by lipopolysaccharide. Biol Pharm Bull 28: 652-656.   DOI   ScienceOn
12 Suh SJ, Chung TW, Son MJ, Kim SH, Moon TC, Son KH, Kim HP, Chang HW, Kim CH. 2006. The naturally occurring biflavonoid, ochnaflavone, inhibits LPS-induced iNOS expression, which is mediated by ERK1/2 via NF-kB regulation, in RAW264.7 cells. Arch Biochem Biophys 447: 136-146.   DOI   ScienceOn
13 Komisseranko NF, Derkuch AI, Kovalyou IP, Bublik NP. 1994. Diterpene glycoside and phenylpropanoid of Stevia rebaudiana bertoni (astercae). Rast Res 1: 53-64.
14 Oshima Y, Saito JI, Hikino H. 1986. Sterebins A, B, C and D, bisnorditerpenoids of Stevia rebaudiana leaves. Tetrahedron 42: 6443-6446.   DOI   ScienceOn
15 Oshima Y, Saito JI, Hikino H. 1988. Sterebins E, F, G and H, diterpenoids of Stevia rebaudiana leaves. Phyochemistry 27: 624-626.   DOI   ScienceOn
16 Dacome AS, da Silva CC, da Costa CEM, Fontana JD, Adelmann J, da Costa SC. 2005. Sweet diterpenic glycoside balance of a new cultivar of Stevia rebaudiana (Bert.) Bertoni: isolation and quantitative distribution by chromatography, spectroscopic, and electrophoretic methods. Process Biochem 40: 3587-3594.   DOI   ScienceOn
17 Lailerd N, Saengsirisuwan V, Sloniger, JA, Toskulkao C, Henriksen E. 2004. Effects of stevioside on glucose transport activity in insulin-sensitive and insulin-resistant rat skeletal muscle. Metabolism 53: 101-107.   DOI   ScienceOn
18 Vallim MA, Barbosa JE, Cavalcanti DN, de Paula JC, da Silva VAGG, Teixeira VL, de Palmer Paixao ICN. 2010. In vitro antiviral activity of diterpenes isolated from the Brazilian brown alga Canstrocarpus cervicornis. J Med Plants Res 4: 2379-2382.
19 Wang BS, Wu JH, Shyur LF, Kuo YH, Chang ST. 2002. Antioxidant activity of abietane-type diterpenes from heartwood of Taiwania cryptomerioides Hayata. Holzforschung 56: 487-492.   DOI
20 Porto TS, Ramgel R, Furtado NAJC, de Carvalho TC, Martins CHG, Veneziani RCS, da Costa FB, Vinholis AHC, Cunha WR, Heleno VCG, Ambrosio SR. 2009. Pimaranetype diterpenes: antimicrobial activity against oral pathogen. Molecules 14: 191-199.   DOI   ScienceOn
21 Liao JF, Chiou WF, Shen YC, Wang GJ, Chen CF. 2011. Anti-inflammatory and anti-infectious effects of Evodia rutaecarpa (Wuzhuyu) and its major bioactive components. Chinese Med 6: 1-8.   DOI   ScienceOn
22 Hoshino T, Tabuchi K, Hara A. 2010. Effects of NSAIDs on the inner ear: possible involvement in cochlear protection. Pharmaceuticals 3: 1286-1295.   DOI
23 Leung AY, Foster S. 1996. Encyclopedia of common natural ingredients used in doods, drugs, and cosmetics. 2th ed. John Wiley & Sons, NY, USA. p 478-480.