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http://dx.doi.org/10.3746/jkfn.2012.41.2.283

Effect of Triacsin C on LPS-induced Inflammation in 3T3-L1 Adipocytes  

Park, Eun-Ju (Dept. of Food & Nutrition, Kyungnam University)
Spurlock, Michael (Dept. of Food Science and Human Nutrition, Iowa State University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.41, no.2, 2012 , pp. 283-288 More about this Journal
Abstract
Triacsin C, an inhibitor of acyl-CoA synthetase, is known to have antiatherosclerotic and vasodilatory activities. The aims of this study were to evaluate the effects of triacsin C on endotoxin-induced (lipopolysaccharide, LPS) inflammation in 3T3-L1 adipocytes and also to evaluate its synergistic effect with triacsin C and resveratrol, a potent antiinflammatory agent. Exposure to LPS for 18 hr increased secretion of IL-6 into the culture medium and mRNA expression of IL-6, MCP-1, TLR and iNOS. Pretreatment of triacsin C for 2 hr suppressed IL-6 accumulation in the medium and the induction of IL-6 expression by LPS, which was more effective than resveratrol treatment. The synergistic effect of triacsin C and resveratrol was found to reduce the expression of iNOS by LPS. However, neither triacsin C nor resveratrol affected the LPS-induced expression of MCP-1, TLR or iNOS. These findings indicate that triacsin C may be a local regulator of inflammation in the adipocyte, although detailed mechanisms are needed to elucidate this through further research.
Keywords
triacsin C; resveratrol; 3T3-L1 adipocytes; pro-inflammatory cytokines;
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1 Namatame I, Tomoda H, Arai H, Inoue K, Omura S. 1999. Complete inhibition of mouse macrophage-derived foam cell formation by triacsin C. J Biochem 125: 319-327.   DOI
2 Weis MT, Crumley JL, Young LH, Stallone JN. 2004. Inhibiting long chain fatty acyl CoA synthetase increases basal and agonist-stimulated NO synthesis in endothelium. Cardiovasc Res 63: 338-346.   DOI   ScienceOn
3 Weis MT, Brady M, Moore M, Crumley J, Stallone JN. 2005. Inhibiting long-chain fatty acyl CoA synthetase does not increase agonist-induced release of arachidonate metabolites from human endothelial cells. J Vasc Res 42: 275-283.   DOI   ScienceOn
4 Lin Y, Lee H, Berg AH, Lisanti MP, Shapiro L, Scherer PE. 2000. The lipopolysaccharide-activated toll-like receptor (TLR)-4 induces synthesis of the closely related receptor TLR-2 in adipocytes. J Biol Chem 275: 24255-24263.   DOI
5 Ajuwon KM, Jacobi SK, Kuske JL, Spurlock ME. 2004. Interleukin-6 and interleukin-15 are selectively regulated by lipopolysaccharide and interferon-γ in primary pig adipocytes. Am J Physiol Regul Integr Comp Physiol 286: R547-R553.   DOI
6 Davis JE, Gabler NK, Walker-Daniels J, Spurlock ME. 2009. The c-Jun N-terminal kinase mediates the induction of oxidative stress and insulin resistance by palmitate and toll-like receptor 2 and 4 ligands in 3T3-L1 adipocytes. Horm Metab Res 41: 523-530.   DOI   ScienceOn
7 Nathan C. 1997. Inducible nitric oxide synthase: what difference does it make? J Clin Invest 100: 2417-2423.   DOI   ScienceOn
8 Centeno-Baez C, Dallaire P, Marette A. 2011. Resveratrol inhibition of inducible nitric oxide synthase in skeletal muscle involves AMPK but not SIRT1. Am J Physiol Endocrinol Metab 301: E922-E930.   DOI
9 Lira FS, Rosa JC, Cunha CA, Ribeiro EB, do Nascimento CO, Oyama LM, Mota JF. 2011. Supplementing alpha-tocopherol (vitamin E) and vitamin D3 in high fat diet decrease IL-6 production in murine epididymal adipose tissue and 3T3-L1 adipocytes following LPS stimulation. Lipids Health Dis 10: 37-41.   DOI
10 Kern PA, Ranganathan S, Li C, Wood L, Ranganathan G. 2001. Adipose tissue tumor necrosis factor and interleukin-6 expression in human obesity and insulin resistance. Am J Physiol Endocrinol Metab 280: E745-E751.   DOI
11 de Pablo MA, Alvarez de Cienfuegos G. 2000. Modulatory effects of dietary lipids on immune system functions. Immunol Cell Biol 78: 31-39.   DOI   ScienceOn
12 Haversen L, Danielsson KN, Fogelstrand L, Wiklund O. 2009. Induction of proinflammatory cytokines by long-chain saturated fatty acids in human macrophages. Atherosclerosis 202: 382-393.   DOI   ScienceOn
13 Lee JY, Sohn KH, Rhee SH, Hwang D. 2001. Saturated fatty acids, but not unsaturated fatty acids, induce the expression of cyclooxygenase-2 mediated through Toll-like receptor 4. J Biol Chem 276: 16683-16689.   DOI
14 Igal RA, Wang P, Coleman RA. 1997. Triacsin C blocks de novo synthesis of glycerolipids and cholesterol esters but not recycling of fatty acid into phospholipid: evidence for functionally separate pools of acyl-CoA. Biochem J 324: 529-534.   DOI
15 Weigert C, Brodbeck K, Staiger H, Kausch C, Machicao F, Haring HU, Schleicher ED. 2004. Palmitate, but not unsaturated fatty acids, induces the expression of interleukin-6 in human myotubes through proteasome-dependent activation of nuclear factor-kappaB. J Biol Chem 279: 23942-23952.   DOI   ScienceOn
16 Staiger H, Staiger K, Stefan N, Wahl HG, Machicao F, Kellerer M, Häring HU. 2004. Palmitate-induced interleukin-6 expression in human coronary artery endothelial cells. Diabetes 53: 3209-3216.   DOI   ScienceOn
17 Ajuwon KM, Spurlock ME. 2005. Palmitate activates the NF-${\kappa}B$ transcription factor and induces IL-6 and TNF-$\alpha$ expression in 3T3-L1 adipocytes. J Nutr 135: 1841-1846.   DOI
18 KCDC. 2010. 2009 Korea national health and nutrition and examination survey. Ministry of health and welfare, Seoul, Korea.
19 Douketis JD, Sharma AM. 2005. Obesity and cardiovascular disease: pathogenic mechanisms and potential benefits of weight reduction. Semin Vasc Med 5: 25-33.   DOI   ScienceOn
20 Matsuzawaa Y, Shimomuraa I, Kiharaa S, Funahashia T. 2003. Importance of adipocytokines in obesity-related diseases. Horm Res 60: 56-59.   DOI
21 Yudkin JS. 2007. Inflammation, obesity, and the metabolic syndrome. Horm Metab Res 39: 707-709.   DOI   ScienceOn
22 Ahn J, Lee H, Kim S, Ha T. 2007. Resveratrol inhibits TNF-alpha-induced changes of adipokines in 3T3-L1 adipocytes. Biochem Biophys Res Commun 364: 972-977.   DOI   ScienceOn
23 Cani PD, Amar J, Iglesias MA, Poggi M, Knauf C, Bastelica D, Neyrinck AM, Fava F, Tuohy KM, Chabo C, Waget A, Delmée E, Cousin B, Sulpice T, Chamontin B, Ferrières J, Tanti JF, Gibson GR, Casteilla L, Delzenne NM, Alessi MC, Burcelin R. 2007. Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes 56: 1761-1772.   DOI   ScienceOn
24 Yu R. 2005. Modulation of obesity-related pathologies based on inflammation theory. Food Industry and Nutrition 10: 5-9.   과학기술학회마을
25 Ajuwon KM, Spurlock ME. 2005. Adiponectin inhibits LPSinduced NF-${\kappa}B$ activation and IL-6 production and increases PPAR ${\gamma}2$ expression in adipocytes. Am J Physiol Regul Integr Comp Physiol 288: R1220-R1225.   DOI   ScienceOn
26 Cao H, Anderson RA. 2011. Cinnamon polyphenol extract regulates tristetraprolin and related gene expression in mouse adipocytes. J Agric Food Chem 59: 2739-2744.   DOI   ScienceOn