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http://dx.doi.org/10.3746/jkfn.2012.41.12.1677

Functionalities of Squid Liver Hydrolysates  

Lee, Su-Seon (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyengsang National University)
Park, Si-Hyang (Sun Marine Biotechnology Co.)
Park, Joo-Dong (Food R&D, CJ Cheiljedang)
Konno, Kunihiko (Lab of Food Biochemistry, Faculty of Fisheries, Hokkaido University)
Choi, Yeung Joon (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyengsang National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.41, no.12, 2012 , pp. 1677-1685 More about this Journal
Abstract
The autolysate and hydrolysate of a common squid liver, Todarodes pacificus, were prepared. Autolysis (liver ratio, pH, temperature) and Protamex-treated hydrolysis (pH, temperature, ratio of protease to liver) conditions were optimized by response surface methodology using central composite design for under 1 hr of hydrolysis time. The desirability profile indicated that maximum DH could be achieved at a squid liver of 93.5%, pH 6.4, and $47^{\circ}C$ in autolysis, while that of Protamex-treated hydrolysis did at a Protamex-to-squid liver level of 0.33%, pH 6.0, and $55^{\circ}C$. Three amino acids, proline, cysteine, and methionine, were not detected in the total amino acid composition of the Protamex-treated hydrolysate, while they were detected in the free amino acid composition. Cadmium was $8.32{\pm}0.03$ mg/100 g-powder for raw, $3.56{\pm}0.02$ mg/100 g-powder for the autolysate, and $13.26{\pm}0.04$ mg/100 g powder for the Protamex-treated hydrolysate. The major molecular weight ranged from 1.0 to 1.5 kDa for the autolysate and from 210 to 470 Da for the Protamex-treated hydrolysate. Food functionalities of the autolysate, such as surface hydrolphobicity, emulsion activity index, emulsion stability, water, and fat adsorption, were similar to the Protamex-treated hydrolysate. Both the autolysate and Protamex-hydrolysate showed high inhibitory activities on the angiotensin-I converting enzyme. Cell toxicity against the HepG2 cell line was not detected in the autolysate or the Protamex-treated hydrolysate by 200 ${\mu}g/mL$.
Keywords
squid liver; autolysate; hydrolysate; functionalities;
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