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http://dx.doi.org/10.3746/jkfn.2011.40.7.949

Macrophage and Anticancer Activities of Feed Additives on β-Glucan from Schizophyllum commune in Breast Cancer Cells  

Lee, Jin-Seok (Dept. of Companion and Laboratory Animal Science, Kongju National University)
Lee, Seung-Ho (Dept. of Companion and Laboratory Animal Science, Kongju National University)
Jang, Yong-Man (QueGen Biotech)
Lee, Jong-Dae (QueGen Biotech)
Lee, Byoung-Hee (National Institute of Biological Resources)
Jung, Ji-Youn (Dept. of Companion and Laboratory Animal Science, Kongju National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.40, no.7, 2011 , pp. 949-955 More about this Journal
Abstract
[ ${\beta}$ ]Glucan is a polysaccharide expressed on the cell walls of fungi. It is known that ${\beta}$-glucan is recognized by a family of C-type lectin receptors, dectin-1, which is expressed mainly on myeloid immune cells, including macrophages, neutrophils and dendritic cells. Raw 264.7 cells were treated with ${\beta}$-glucan from Schizophyllum commune. ${\beta}$-Glucan was not cytotoxic up to 400 ${\mu}g$/mL as measured by MTT assay. To measure the activity of macrophages, NO and TNF-${\alpha}$ assays were performed in Raw 264.7 cells. Treatment with ${\beta}$-glucan for 24 hr significantly increased production of NO and TNF-${\alpha}$ compared with control groups (p<0.05), indicating activation of macrophages. To measure inhibition of breast cancer cell proliferation, MTT assay was performed in MDA-MB-231 cells. Cell viability was significantly decreased in the group treated with 400 ${\mu}g$/mL of ${\beta}$-glucan for 48 hr (p<0.05) compared to the control group. However, tumor volume was decreased in the groups administered 200 ${\mu}g$ of ${\beta}$-glucan/mouse compared to the control group. These results indicate that ${\beta}$-glucan inhibits breast cancer cell growth through the induction of apoptosis.
Keywords
${\beta}$-glucan; macrophage; anticancer; breast cancer; Schizophyllum commune;
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