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http://dx.doi.org/10.3746/jkfn.2011.40.5.649

Induction of Apoptosis in Human Colon Carcinoma HCT116 Cells Using a Water Extract of Lepidium virginicum L.  

Chae, Yang-Hui (Dept. of Biology, Kyungsung University)
Shin, Dong-Yeok (Dept. of Biochemistry, Dongeui University College of Oriental Medicine)
Park, Cheol (Dept. of Biochemistry, Dongeui University College of Oriental Medicine)
Lee, Yong-Tae (Dept. of Pathology, Research Institute of Oriental Medicine, Dongeui University College of Oriental Medicine)
Moon, Sung-Gi (Dept. of Biology, Kyungsung University)
Choi, Yung-Hyun (Dept. of Biochemistry, Dongeui University College of Oriental Medicine)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.40, no.5, 2011 , pp. 649-659 More about this Journal
Abstract
To examine the anti-cancer effects of Lepidium virginicum L., the anti-proliferative and pro-apoptotic effects of a water extract of L. virginicum leaves (WELVL) and of L. virginicum roots (WELVR) were investigated in HCT116 human colon carcinoma cells. The treatment of HCT116 cells with WELVL and WELVR resulted in the inhibition of growth and morphological changes in a concentration-dependent manner by inducing apoptosis. The growth inhibition and apoptosis induction by WELVR was stronger than that of WELVL thus, we determined that WELVR was the more optimal extract for this study. The increased apoptotic events in HCT116 cells caused by WELVR were associated with an up-regulation of Fas ligand, Bax, and Bad expression, a down-regulation of Bcl-2, Bcl-$_XL$, and Bid expression, and a decrease in the mitochondrial membrane potential (MMP, ${\Delta}{\psi}m$). WELVR treatment induced the proteolytic activation of caspase-3, -8, and -9, and the degradation of caspase-3 substrate proteins, such as poly (ADP-ribose) polymerase (PARP), ${\beta}$-catenin, and phospholipase C-${\gamma}1$ (PLC-${\gamma}1$). In addition, apoptotic cell death induced by WELVR was correlated with a down-regulation of inhibitors of the apoptosis protein (IAP) family, such as the X-linked inhibitor of apoptosis protein (XIAP), cIAP-1, and cIAP-2. These findings suggest that the WELVR-induced inhibition of cell proliferation is associated with the induction of apoptotic cell death. WELVR may be a potential chemotherapeutic agent for the control of HCT116 human colon carcinoma cells.
Keywords
Lepidium virginicum L.; HCT116; apoptosis; caspase;
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