Browse > Article
http://dx.doi.org/10.3746/jkfn.2011.40.5.635

Suppressive Effects of Ethyl Acetate Fraction from Green Tea Seed Coats on the Production of Cell Adhesion Molecules and Inflammatory Mediators in Human Umbilical Vein Endothelial Cells  

Noh, Kyung-Hee (School of Foods and Life Science and Institute of Basic Sciences, Inje University)
Kim, Jong-Kyung (School of Foods and Life Science and Institute of Basic Sciences, Inje University)
Song, Young-Sun (School of Foods and Life Science and Institute of Basic Sciences, Inje University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.40, no.5, 2011 , pp. 635-641 More about this Journal
Abstract
Anti-atherogenic effects in tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$)-stimulated human umbilical vein endothelial cells (HUVEC) are involved with suppressed oxidative stress, cell adhesion molecules, and pro-inflammatory factors. The aim of this study was to determine whether green tea seed coat ethyl acetate fraction (GTSCE) could modulate cell adhesion molecules and inflammatory mediators in HUVEC stimulated with TNF-${\alpha}$. Nitric oxide (NO) production was significantly increased in TNF-${\alpha}$-stimulated HUVEC compared to TNF-${\alpha}$ only treated cells. The NO that is produced by endothelial nitric oxide synthase dilates blood vessels and has protective effects against platelet and leucocyte adhesion. GTSCE at 25, 50, 75, and $100\;{\mu}g$/mL significantly (p<0.05) reduced TNF-${\alpha}$ production. GTSCE significantly (p<0.05) inhibited soluble vascular cell adhesion molecule-1 level, in a dose-dependent manner. Monocyte chemoattractant protein-1 level was also significantly (p<0.05) inhibited by GTSCE treatment at $75\;{\mu}g$/mL compared to the TNF-${\alpha}$-only treated group. Total antioxidant capacity by GTSCE was significantly (p<0.05) enhanced compared to the TNF-${\alpha}$-only treated group. These results suggest that GTSCE can inhibit the production of cell adhesion molecules and inflammatory mediators and could be used as a candidate bioactive material to prevent the development of atherosclerosis.
Keywords
HUVEC; green tea seed coat; pro-inflammatory mediators; cell adhesion molecules;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Hayashi E, Hayashi M, Yamazoe H. 1990. Pharmacological action of tea extracts on the central nervous system in mice. Oyo Yakuri 40: 351-356.
2 Kim MJ, Choi JH, Yang JA, Kim SY, Kim JH, Lee JH, Kim JK, Rhee SJ. 2002. Effects of green tea catechin on enzyme activities and gene expression of antioxidative system in rat liver exposed to microwaves. Nutr Res 22: 733-744.   DOI
3 Cho SY, Oh YJ, Park JY, Lee MK, Kim MJ. 2003. Effect of dandelion leaf extracts on (Taraxacum officinale) hepatic antioxidative system in rats fed high cholesterol diet. J Korean Soc Food Sci Nutr 32: 458-463.   과학기술학회마을   DOI
4 Nakayama T, Yoshizaki A, Izumida S, Suehiro T, Miura S, Uemura T, Yakata Y, Shichijo K, Yamashita S, Sekin I. 2007. Expression of interleukin-11 (IL-11) and IL-11 receptor alpha in human gastric carcinoma and IL-11 upregulates the invasive activity of human gastric carcinoma cells. Int J Oncol 30: 825-833.
5 Ozer N, Tangurek B, Firat F, Ozer S, Tartan Z, Ozturk R, Ozey B, Ciloglu F, Yilmaz H, Cam N. 2008. Effects of drug-eluting stents on systemic inflammatory response in patients with unstable angina pectoris undergoing percutaneous coronary intervention. Heart Vessels 23: 75-82.   DOI
6 Churg A, Dai J, Tai H, Xie C, Wright JL. 2002. Tumor necrosis factor-alpha central to acute cigarette smoke-induced inflammation and connective tissue breakdown. Am J Respir Crit Care Med 166: 849-854.   DOI
7 Ohta H, Wada H, Niwa T, Kirii H, Iwamoto N, Fuji H, Saito K, Seishima M. 2005. Disruption of tumor necrosis factor-alpha gene diminishes the development of atherosclerosis in ApoE-deficient mice. Atherosclerosis 180: 11-17.   DOI
8 Finkel T, Holbrook JN. 2000. Oxidants, oxidative stress and the biology of aging. Nature 408: 239-249.   DOI
9 Gao X, Xu X, Belmadani S, Park Y, Tand Z, Feldman A, Chilian W, Zhang C. 2007. TNF-alpha contributes to endothelial dysfunction by upregulating arginase in ischemia/reperfusion injury. Arterioscler Thromb Vasc Biol 27: 1269-1275.   DOI
10 Maekawa Y, Ishikawa K, Yasuda O, Oguro R, Hanasaki H, Kida I, Takemura Y, Ohishi M, Katsuya T, Rakugi H. 2009. Klotho suppresses TNF-alpha-induced expression of adhesion molecules in the endothelium and attenuates NF-kappa B activation. Endocrine 35: 341-346.   DOI
11 Rong Y, Geng Z, Lau BH. 1996. Ginko biloba attenuates oxidative stress in macrophages and endothelial cells. Free Radic Biol Med 20: 121-127.   DOI
12 Stein O, Thiery J, Stein Y. 2002. Is there a genetic basis for resistance to atherosclerosis? Atherosclerosis 160: 1-10.   DOI
13 Lee CH, Yi HS, Kim JE, Heo SK, Cha CM, Won CW, Park SD. 2009. Anti-oxidative and anti-inflammatory effect of fractionated extracts of Smilacis glabrae rhizoma in human umbilical vein endothelial cell. Kor J Herbology 24: 39-50.   과학기술학회마을
14 Mohamed F, Monge JC, Gordon A, Cernacek P, Blais D, Stewart DJ. 1995. Lack of role for nitric oxide (NO) in the selective destabilization of endothelial NO synthase mRNA by tumor necrosis factor-$\alpha$. Arterioscler Tromb Vasc Biol 15: 52-57.   DOI   ScienceOn
15 Vallance P, Collier J. 1994. Biology and clinical relevance of nitric oxide. Br Med J 309: 453-457.   DOI   ScienceOn
16 Kim TH, Ko SS, Park C, Park SE, Hong SH, Kim BW, Choi YH. 2010. Anti-inflammatory effects of Nerium indicum ethanol extracts through suppression of NF-kappaB activation. J Life Sci 20: 1221-1229.   DOI
17 Lee HH, Park C, Kim MJ, Seo MJ, Choi SH, Jeong YK, Choi YH. 2010. Inhibition of cyclooxygenase-2 activity and prostaglandin $E_2$ production through down-regulation of NF-kB activity by the extracts of fermented beans. J Life Sci 20: 388-395.   과학기술학회마을   DOI
18 Kim EH, Rhee DK. 2009. Anti-oxidative properties of ginseng. J Ginseng Res 33: 1-7.   과학기술학회마을   DOI
19 Balzary RW, Cocks TM. 2006. Lipopolysaccharide induces epithelium- and prostaglandin E(2)-dependent relaxation of mouse isolated trachea through activation of cyclooxygenase (COX)-1 and COX-2. J Pharmacol Exp Ther 317:806-812.   DOI
20 Lee EM, Choi YJ, Lee J, Ku L, Kim KH, Choi JS, Lim SJ. 2006. $\alpha$-Tocopheryl succinate, in contrast to $\alpha$-tocopherol and $\alpha$-tocopheryl acetate, inhibits prostaglandin $E_2$ production in human lung epithelial cells. Carcinogenesis 27: 2308-2315.   DOI
21 Seals DF, Courtneidge SA. 2003. The ADAMs family of metalloproteases: multidomain proteins with multiple functions. Genes Dev 17: 7-30.   DOI
22 Xu B, Nakhla S, Makris A, Hennessy A. 2011. TNF-$\alpha$ inhibits trophoblast integration into endothelial cellular networks. Placenta 32: 241-246.   DOI
23 Yoon WH, Choi JH, Lee KH, Kim CH. 2005. Antimicrobial and antitumor activities of seed extracts of Camellia sinensis L. Korean J Food Sci Technol 37: 108-112.   과학기술학회마을
24 Park JS, Rho HS, Kim DH, Chang IS. 2006. Enzyme preparation of kaempferol from green tea seed and its antioxidant activity. J Agric Food Chem 54: 2951-2956.   DOI
25 Rah HH, Baik SO, Han SB, Bock JY. 1992. Chemical compositions of the seed of Korean green tea plant (Camellia sinecis L.). J Korean Agric Chem Soc 35: 272-275.   과학기술학회마을
26 Yosioka I, Nishimura T, Matsuda A, Kitagawa I. 1970. Saponin and sapogenol. II. Seeds sapogenols of Thea sinensis L. Theasapogenol A. Chem Pharm Bull 18: 1621-1632.   DOI
27 Mnocada S, Palmer RM, Higgs EA. 1991. Nitric oxide: physiology, pathophysiology and pharmacology. Pharmacol Rev 43: 109-142.
28 Bernas T, Dobrucki J. 2002. Mitochondrial and nonmitochondrial reduction of MTT: interaction of MTT with TMRE, JC-1, and NAO mitochondrial fluorescent probes. Cytometry 47: 236-242.   DOI
29 Green LC, Wagner DA, Glogowski J. 1982. Analysis of nitrate, nitrite, and [$15^N$]nitrate in biological fluids. Anal Biochem 126: 131-138.   DOI
30 Erel O. 2004. A novel automated method to measure total antioxidant response against potent free radical reaction. Clin Biochem 37: 112-119.   DOI
31 Ignarro LJ, Cirino G, Casini A, Napoli C. 1999. Nitric oxide as signaling molecule in the vascular system: an overview. J Biol Chem 34: 879-886.
32 Marsden PA, Schappert KT, Chen HS, Flowers M, Sundell CL, Wilcox JN, Lamas S, Michel T. 1992. Molecular clonning and characterization of human endothelial nitric oxide synthase. FBSS Lett 307: 287-293.   DOI
33 Yun HJ, Heo SK, Lee YT, Park WH, Park SD. 2008. Antiinflammatory effect of Evodia officinalis DODE in mouse macrophage and human vascular endothelial cells. Kor J Herbology 23: 29-38.
34 Stenvinkel P. 2003. Interactions between inflammation, oxidative stress, and endothelial dysfunction in end-stage renal disease. J Ren Nutr 13: 144-148.   DOI
35 Libby P. 2002. Inflammation in atherosclerosis. Nature 420: 868-874.   DOI
36 Ito T, Ikeda U. 2003. Inflammatory cytokines and cardiovascular disease. Curr Drug Targets Inflamm Allergy 2: 257-265.   DOI
37 Riehemann K, Behnke B, Schulze-Osthoff K. 1999. Plant extracts from stinging nettle (Urtica dioica), an antirheumatic remedy, inhibit the proinflammatory transcription factor NF-${\kappa}B$. FEBS Lett 442: 89-94.   DOI
38 Cheon MS, Yoon T, Choi G, Kim SJ, Lee AY, Moon BC, Choo BK, Kim HK. 2009. Comparative study of extracts from rhubarb on inflammatory activity in RAW 264.7 cells. Korean J Medicinal Crop Sci 17: 109-114.   과학기술학회마을
39 Li JJ, Zhu CG, Yu B, Liu YX, Yu MY. 2007. The role of inflammation in coronary artery calcification. Ageing Res Rev 6: 263-270.   DOI
40 Lawrence T, Gilroy DW, Colville-Nash PR. 2001. Possible new role for NF-${\kappa}B$ in the resolution of inflammation. Nat Med 7: 1291-1297.   DOI
41 Allenm R, Tresini M. 2000. Oxidative stress and gene regulation. Free Radic Biol Med 28: 463-499.   DOI
42 Lee HB, Kim EK, Park SJ, Bang SG, Kim TG, Chung DW. 2010. Isolation and characterization of nicotiflorin obtained by enzymatic hydrolysis of two precursors in tea seed extract. J Agric Food Chem 58: 4808-4813.   DOI
43 Guha M, Mackman N. 2001. LPS induction of gene expression in human monocytes. Cell Signal 13: 85-94.   DOI
44 Madamanchi NR, Vendrov A, Runge MS. 2005. Oxidative stress and vascular disease. Arterioscler Thromb Vasc Biol 25: 29-38.
45 Mariathasan S, Monack DM. 2007. Inflammation adaptors and sensors; intracellular regulators of infection and inflammation. Nat Rev Immunol 7: 31-40.   DOI
46 Zedler S, Faist E. 2006. The impact of endogenous triggers on trauma-associated inflammation. Curr Opin Crit Care 12: 595-560.   DOI