Browse > Article
http://dx.doi.org/10.3746/jkfn.2011.40.1.089

Changes in Properties of Deer Antler by Proteolysis and Extraction Conditions  

Kim, Jae-Hwa (BOT Co., Ltd.)
Yoo, Cheol-Jae (BOT Co., Ltd.)
Sin, Kyung-A (Keimyung Foodex Co., Ltd.)
Jang, Se-Young (Keimyung Foodex Co., Ltd.)
Park, Nan-Young (Keimyung Foodex Co., Ltd.)
Jeong, Yong-Jin (Dept. of Food Science and Technology, Keimyung University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.40, no.1, 2011 , pp. 89-93 More about this Journal
Abstract
This study was conducted to investigate the proteolysis and extraction conditions of deer antler for application of food materials. ProteAX (A) was the most effective enzyme for proteolysis of deer antler and the proteolysis condition was 0.5% (w/w) for enzyme concentration and 5 hr for proteolysis time. The effect of mixing enzyme ProteAX (A)+KFEN 2 (C) treatment in $60^{\circ}C$, 5 hr was investigated; soluble solid and protein content were the highest with A 0.5% (w/w) and B 0.5% (w/w) concentration. Result for DAH (deer antler hydrolysate) and DA (deer antler) prepared with extraction in $95^{\circ}C$ atmospheric pressure (AP, 6~18 hr) and extraction under $120^{\circ}C$ pressure condition (UP, 15~60 min) after hydrolysis on preceding established condition descriptions indicated that difference in pH according to enzyme treatment and extraction conditions was not significant. Sugar content of DA was $1.5^{\circ}Brix$, DA-UP (under pressure) and DAH-AP (atmospheric pressure) were $2.2^{\circ}Brix$; the highest sugar content of $2.7^{\circ}Brix$ was observed in DAH-UP for 60 min extraction. Also total free sugar, crude protein and collagen content were the highest in DAH-UP for 60 min recording at 1.97%, 742.7 mg/100 g and 498.8 mg/100 g, respectively. From these results, deer antler hydrolysate prepared with extraction under pressure was the most effective for functional characteristics enhancement. Hereafter, various practical uses of materials with enhanced characteristics of antler is expected.
Keywords
deer antler; proteolysis; proteolytic enzyme; collagen; extraction;
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Shin KH, Lim SS, Lee SH, Lee YS, Cho SY. 2001. Antioxidant and immunostimulating activities of the fruiting bodies of Paecilomyces japonica, a new type of Cordyceps sp. Ann N Y Acad Sci 928: 261-273.
2 Kim YT, Kim CS. 1996. A study of hematopoietic action of pilose antler in senescence accelerated mice. Kor J Pharmacogn 27: 371-377.   과학기술학회마을
3 Sin GH. 2002. Deer activity and the active ingredients of the medication. The first KNRC Nokyong Science Symposium-Scientific understanding of deer. Cheongju, Korea. p 55-87.
4 Kwak HB, Lee SW, Li YJ, Kim YA, Han SY, Jhon GJ, Kim HH, Lee ZH. 2004. Inhibition of osteoclast differentiation and bone resorption by a novel lysophosphatidylcholine derivative, SCOH. Biochem Pharmac 67: 1239-1248.   DOI
5 Shin KH, Lim EB, Kim JH, Chung MS, Cho SI. 1989. Pharmacological studies on powered whole part of unossified antler. Kor J Pharmcogn 20: 180-187.
6 Park PJ, Jeon YJ, Moon HS, Jeon TB. 2005. Chemical composition and biological activity of velvet antler. Food Industry and Nutrition 10: 50-59.   과학기술학회마을
7 Lee BY, Lee OH, Choi HS. 2003. Analysis of food components of Korean deer antler parts. Korean J Food Sci Technol 35: 52-56.   과학기술학회마을
8 Kim DH, Han SB, Park JS, Han MJ. 1994. Fermentation of antler and its biological activity. Kor J Pharmacogn 25: 233-237.   과학기술학회마을
9 Choi KH, Um CI. 1994. Process for preparing antler extracts. Korean patent 2011.
10 Lee DS. 2008. Method of preparing deer velvet extract. Korean patent 10568.
11 Jeon KJ, Woo YJ, Kim JK. 2002. Extract of deer antler containing bone formation enhancing activity and its purifying method. Korean patent 6876.
12 Qi SB, Zhao XH, Yang XW, Hattori M, Namba T, Nomura Y. 1988. Effect of ethanol extract of Rokujo, pilose antler, on uptake and release of noradrenal in cerebral cortical slices of rats. J Med Pharm Soc For Wakanyaku 5: 93-97.
13 Wang BX, Zhao XH, Yang XY, Kaneko S, Hattori M, Namba T, Nomura Y. 2001. Inhibition of lipid peroxidation by deer antler (Rokujo) extract in vivo and in vitro. J Med Pharm Soc For Wakanyaku 5: 123-128.
14 Lee WB. 2007. Study on the free radical scavenging activity of enzymatic extracts from velvet antler of elk (Cervus Canadensis). MS Thesis. Konkuk University, Seoul, Korea. p 1-3.
15 Lee SH, Cho YJ, Kim S, Ahn BJ, Choi C. 1995. Optimal conditions for the enzymatic hydrolysis of isolated sesame meal protein. J Appl Biol Chem 38: 248-253.   과학기술학회마을
16 Ann YG. 2003. Extraction of freeze dried young antler by water and protease. Korean J Food & Nutr 16: 379-387.   과학기술학회마을
17 Pommer K. 1995. New proteolytic enzymes for the production of savory ingredients. Cereal Foods World 40: 745-748.
18 Baek IB. 2002. A process for preparing antler powder. Korean patent 1557.
19 Kim MK, Jung EY, Lee HS, Shin KS, Kim YK, Ra KS, Park CS, Woo MJ, Lee SH, Kim JS, Suh HJ. 2009. Isolation of strain for the preparation of the fermented antler and its physiological activities. J Korean Soc Food Sci Nutr 38: 1237-1242.   과학기술학회마을   DOI
20 Ann YG. 2004. Extraction of young antler and antler by water, proteases and HCl. Korean J Food & Nutr 17: 147-155.   과학기술학회마을
21 Yeo IH, Lee SY, Park JM. 2002. The process method of concentrated powder for enzyme treatment in Cervi Parvum Corun. Korean patent 14395.
22 An JH. 2002. Hydrolysis manufacture method of buck's cornu. Korean patent 65197.
23 Gu YA, Jang SY, Park NY, Mun CR, Kin OM, Jeong YJ. 2006. Property changes of mung bean depending on hydrolysis conditions. Korean J Food Preserv 13: 563-568.   과학기술학회마을
24 Kim KY, Shin JK, Lee SW, Yoon SR, Chung JS, Jeong YJ, Choi MS, Lee CM, Moon KD, Kwon JH. 2007. Quality and functional properties of red ginseng prepared with different steaming time and drying methods. Korean J Food Sci Technol 39: 494-499.   과학기술학회마을
25 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-271.
26 Kolar K. 1990. Colorimetric determination of hydroxyproline as measure of collagen content in meat and products. NMKL Collaborative Study. J Assoc Off Anal Chem 73: 55-60.