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http://dx.doi.org/10.3746/jkfn.2010.39.9.1263

Antioxidative and Anti-asthma Effect of Morus Bark Water Extracts  

Kim, Jeong-Mi (Dept. of Bio-Health Technology, Kangwon National University)
Baek, Jong-Mi (Dept. of Bio-Health Technology, Kangwon National University)
Kim, Hyun-Sook (Well-being Bioproducts RIC Center, Kangwon National University)
Choe, Myeon (Dept. of Bio-Health Technology, Kangwon National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.9, 2010 , pp. 1263-1269 More about this Journal
Abstract
This study was performed to evaluate the antioxidant activities and anti-asthma effects of Morus bark water extracts. Inhibitory effect of Morus bark onto free radical generation was determined by measuring DPPH and hydroxyl radical scavenging activities in vitro. Anti-asthma activities of Morus bark water extracts were assessed by testing their effects on the degranulation of mast cell. For this, $\beta$-hexosaminidase released from a basophilic cell line, RBL-2H3 was used and pro-inflammatory cytokines were measured by ELISA kit. The antioxidant activities of water extracts of Morus bark was 59.2% in the DPPH assay at $2,000\;{\mu}g/mL$ and 78.8% in the hydroxyl radical scavenging assay at $2,000\;{\mu}g/mL$. Our results indicated that Morus bark water extracts effectively inhibited free radical generation. Morus bark water extracts inhibited inflammation-mediating substances such as histamine and $\beta$-hexosaminidase release from RBL-2H3 cells. Cytokine release demonstrated a more effective blockading ability of the Morus bark water extracts to the release of IL-4 and TNF-$\alpha$ compared to control. These results demonstrate that Morus bark may be beneficial in the treatment of allergic inflammatory disease.
Keywords
Morus bark; inflammation; RBL-2H3; IL-4; TNF-$\alpha$; $\beta$-hexosaminidase;
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