Browse > Article
http://dx.doi.org/10.3746/jkfn.2010.39.8.1156

Synthesis and Characterization of Structured Lipids from Evening Primrose Seeds Oil and Rice Bran Oil  

Kim, Hyo-Jin (Dept. of Food Science and Technology, Chungnam National University)
Lee, Kyung-Su (Dept. of Food Science and Technology, Chungnam National University)
Lee, Ki-Teak (Dept. of Food Science and Technology, Chungnam National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.8, 2010 , pp. 1156-1164 More about this Journal
Abstract
Structured lipids (SLs) were synthesized by enzymatic interesterification with evening primrose oil (EPO) and rice bran oil (RBO) in a batch-type reactor. The interesterification was performed using a water shaker for 24 hr at $55^{\circ}C$. Mixing speed was set at 200 rpm and Lipozyme RM IM (immobilized lipase from Rhizomucor miehei, 10% by weight of total substrates) was used as a biocatalyst. Rice bran oil and evening primrose oil were interesterified with various molar ratios (RBO : EPO, 1:3, 1:4, and 1:5 mol/mol). Reversed-phase high performance liquid chromatography connected with evaporative light-scattering detector was performed to separate the triacylglycerol (TAG) species of SLs. In the fatty acid analysis, $\gamma$-linolenic acid (7.9 mol%), linoleic acid (67.3 mol%) and oleic acid (13.2 mol%) were the most abundant fatty acids in the SLs. During 24 hr reaction, most of the reaction occurred within 3 hr. TAG compositions, tocopherols and phytosterols were also analyzed. In the TAG species analysis, LLL (ECN=42, L=linoleic acid) dramatically decreased when the reaction time increased.
Keywords
structured lipids; rice bran oil; evening primrose oil;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Hudson BJF. 1984. Evening primrose (Oenothera Spp.) oil and seed. J Am Oil Chem Soc 61: 540-543.   DOI
2 Tatematsu K, Fuma S, Nagase T, Ichikawa Y, Fujii Y. 2004. Factors other than phytosterols in some vegetable oils affect the survival of SHRSP rats. Food Chem Toxicol 42: 1443-1451.   DOI
3 Mariod A, Matthaus B, Eichner K. 2004. Fatty acid, tocopherol and sterol composition as well as oxidative stability of three unusual Sudanese oils. J Food Lipids 11: 179-189.   DOI   ScienceOn
4 Miller A, Frenzel T, Schmarr HG, Engel KH. 2003. Coupled liquid chromatography-gas chromatography for the rapid analysis of $\gamma$-oryzanol in rice lipids. J Chromatogr A 985:403-410.   DOI
5 Woo KM, Lee YS, Kim YH. 2007. Effect of dietary tocotrienol extracted from rice bran on hematological and histological changes of the mouse. J Korean Plant Res 20: 104-112.
6 Lee KT, Akoh CC. 1998. Structured lipid: synthesis and applications. Food Rev Int 14: 17-34.   DOI
7 Watanabe T, Sugiura M, Sato M, Yamada N, Nakanishi K. 2005. Diacylglycerol production in a packed bed bioreactor. Process Biochem 40: 637-643.   DOI
8 KFDA. 2009. Department of Food Standardization, labeling of trans fats. Korea Food and Drug Administration, Seoul, Korea.
9 Fomuso LB, Akoh CC. 2002. Lipase-catalyzed acidolysis of olive oil and caprylic acid in a bench-scale packed bed bioreactor. Food Res Int 35: 15-21.   DOI
10 William WC. 1987. High-performance liquid chromatography and lipids: a Practical Guide. Pergamon Press, Oxford, England. p 169-185.
11 Lee KT, Foglia TA. 2001. Fractionation of menhaden oil and partially hydrogenated menhaden oil; characterization of triacylglycerol fractions. J Am Oil Chem Soc 78: 297-303.   DOI
12 Hall B. 2009. Uniformity of human milk. Am J Clin Nutr 32: 304-312.
13 Kim JY, Lee KT. 2009. Enzymatic synthesis of low trans fats using rice bran oil, palm stearin and high oleic sunflower seed oil. J Korean Soc Food Sci Nutr 38: 470-478.   과학기술학회마을   DOI
14 Shimizu S, Nakano M. 2003. Structural characterization of triacylglycerol in several oils containing gamma-linolenic acid. Biosci Biotechnol Biochem 67: 60-67.   DOI
15 Makrides M, Gibson RA. 2009. Long-chain polyunsaturated fatty acid requirements during pregnancy and lactation. Am J Clin Nutr 71: 307-311.
16 Michael Eskin NA. 2008. Borage and evening primrose oil. Eur J Lipid Sci Technol 110: 651-654.   DOI
17 Kang HK, Park BS. 2007. Evening primrose oil and hemp seed oil as an $\gamma$-linolenic acid source for broiler: influence of fatty acid composition of chicken skin, thigh and breast muscle. J Korean Oil Chem Soc 24: 196-204.   과학기술학회마을
18 Harbige LS. 1998. Dietary n-6 and n-3 fatty acids in immunity and autoimmune disease. Proc Nutr Soc 57: 555-562.   DOI
19 Leizer C, Ribnicky D, Poulev A, Dushenkov S, Raskin I. 2000. The composition of hempseed oil and its potential as important source of nutrition. J Nutr Func Med Foods 2: 35-53.   DOI
20 Oomash B, Busson DM, Godfrey DV. 2002. Characteristics of hemp (Cannabis sativa L.) seed oil. Food Chem 76: 33-43.   DOI
21 Nergiz C, Donmez I. 2004. Chemical composition and nutritive value of Pinus pinea L. seeds. Food Chem 86: 365-368.   DOI
22 Grigg D. 1999. The fats of the land: a geography of oil and fat consumption. Geojournal 48: 259-268.   DOI
23 Nam SH, Cho SP, Kang MY, Koh HJ, Kozukue N, Friedman M. 2006. Antioxidative activities of bran extracts from twenty one pigmented rice cultivars. Food Chem 94: 613-620.   DOI
24 Xu Z, Hua N, Godber JS. 2001. Antioxidant activity of tocopherols, tocotrienols, and $\gamma$-oryzanol components from rice bran against cholesterol oxidation accelerated by 2,2'-azobis (2-methylpropinamine) dihydrochloride. J Agric Food Chem 49: 2077-2081.   DOI
25 Park BS. 2008. Gamma fatty acid: a review. J Korean Oil Chem Soc 25: 446-458.   과학기술학회마을
26 Fan YY, Chapkin RS. 1998. Importance of dietary $\gamma$-linolenic acid in human health and nutrition. J Nutr 128: 1411-1414.
27 Emelyanova EV. 1997. Lipid and $\gamma$-linolenic acid production by Mucor inaquisporus. Pro Biochem 32: 173-177.   DOI
28 Barre DE. 2001. Potential of evening primrose, borage, black currant, and fungal oils in human health. Ann Nutr Metab 45: 47-57.   DOI
29 Park BS. 2008. Effect of feeding evening primrose oil and hemp seed oil on the deposition of gamma fatty acid in eggs. J Korean Oil Chem Soc 25: 166-204.