Browse > Article
http://dx.doi.org/10.3746/jkfn.2010.39.8.1126

Antiobesity Effect of the Bacillus subtilis KC-3 Fermented Soymilk in 3T3-L1 Adipocytes  

Kim, Ji-Young (Dept. of Food Science and Nutrition, Pusan National University)
Jeong, Jung-Eun (Dept. of Food Science and Nutrition, Pusan National University)
Moon, Suk-Hee (Dept. of Subdivision of Food Nutrition & Bakery, Kyungnam College of Information and Technology)
Park, Kun-Young (Dept. of Food Science and Nutrition, Pusan National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.8, 2010 , pp. 1126-1131 More about this Journal
Abstract
The antiobesity effect of soymilks fermented with Bacillus subtilis KC-3 (KCCM 42923) from cheonggukjang was compared with other sources of B. subtilis KCCM 11316 and B. subtilis MYCO. The antiobesity effect was investigated by measuring the release of leptin, Oil red O staining, glycerol secretions and adipogenic transcription factor by reverse transcription-polymerase chain reaction (RT-PCR) in the 3T3-L1 adipocytes. Fermented soymilk with B. subtilis KC-3 (F-KC) led to decrease levels of leptin secretion and increase levels of glycerol secretion in the cells. In addition, F-KC reduced contents of Oil red O dye in the 3T3-L1 adipocytes. Also, mRNA expression levels of both SREBP-1c (sterol regulatory element-binding protein 1-c) and PPAR-$\gamma$ (peroxisome proliferator-activated receptor-$\gamma$), which are adipogenic transcription factor, in cells treated with F-KC were markedly down regulated. These results demonstrate that the Bacillus subtillis fermented soymilk (F-KC) decreased lipid content in 3T3-L1 adipocytes by inhibiting lipogenesis. All B. subtilis fermented soymilks had shown antiobesity activities, however, F-KC exhibited the strongest antiobesity effect in the 3T3-L1 adipocytes. Our study suggests that especially F-KC increased the potential of antiobesity effects.
Keywords
fermented soymilk; Bacillus subtilis; antiobesity; 3T3-L1;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Tsai TY, Chu LH, Lee CL, Pan TM. 2009. Atherosclerosispreventing activity of lactic acid bacteria-fermented milksoymilk supplemented with Momordica charantia. J Agric Food Chem 57: 2065-2071.   DOI
2 Jo JW, Hur SH, Han YS, Kim JY. 2009. Isolation of lipase producing Bacillus subtilis and some characteristics of the enzyme. J Appl Biol Chem 52: 151-156.   DOI
3 Park JY, Kim JB. 2002. Molecular insights into fat cell differentiation and functional roles of adipocytokines. J Korean Soc Endocrinol 17: 1-9.
4 Rosen ED, Sarraf P, Troy AE, Bradwin G, Moore K, Milstone DS, Spiegelman BM, Mortensen RM. 1999. PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro. Mol Cell 4: 611-617.   DOI
5 Horton JD, Goldstein JL, Brown MS. 2002. SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Invest 109: 1125-1131.   DOI
6 Fajas L, Schoonjans K, Gelman L, Kim JB, Najib J, Martin G, Fruchart JC, Briggs M, Spiegelman BM, Auwerx J. 1999. Regulation of peroxisome proliferator-activated receptor $\gamma$ expression by adipocyte differentiation and determination factor 1/sterol regulatory element binding protein 1: implications for adipocyte differentiation and metabolism. Mol Cell Biol 19: 5495-5503.   DOI
7 Kim JB, Spiegelman BM. 1996. ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism. Genes Dev 10: 1096-1107.   DOI
8 Frost SC, Lane MD. 1985. Evidence for the involvement of vicinal sulfhydryl groups in insulin-activated hexose transport by 3T3-L1 adipocytes. J Biol Chem 260: 2646-2652.
9 Mac Dougald OA, Hwang CS, Fan H, Lane MD. 1995. Regulated expression of the obese gene product (leptin) in white adipose tissue and 3T3-L1 adipocytes. Proc Natl Acad Sci USA 20: 9034-9037.
10 Green H, Kehinde O. 1975. An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion. Cell 5: 19-27.   DOI
11 Campfield LA, Smith FJ, Guisez Y, Devos R, Burn P. 1995. Recombinant mouse OB protein: evidence for a peripheral signal linking adiposity and central neural networks. Science 269: 546-549.   DOI
12 Palous A, Serra F, Bonet ML, Pico C. 2000. Obesity: molecular bases of a multifactorial problem. Eur J Nutr 3: 127-144.
13 Slavin BG, Ong JM, Kern PA. 1994. Hormonal regulation of hormone-sensitive lipase activity and mRNA levels in isolated rat adipocytes. J Lipid Res 35: 1535-1541.
14 Norman D, Isidori AM, Frajese V, Caprio M, Chew SL, Grossman AB, Clark AJ, Michael Besser G, Fabbri A. 2003. ACTH and alpha-MSH inhibit leptin expression and secretion in 3T3-L1 adipocytes: model for a central-peripheral melanocortin-leptin pathway. Mol Cell Endocrinol 200: 99-109.   DOI
15 Maffei M, Halaas J, Ravussin E, Pratley RE, Lee GH, Zhang Y, Fei H, Kim S, Lallone R, Ranganathan S. 1995. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nat Med 1: 1155-1161.   DOI
16 Havel PJ. 2000. Role of adipose tissue in body-weight regulation:mechanisms regulating leptin production and energy balance. Proc Nutr Soc 59: 359-371.   DOI
17 Kim SH, Yang JL, Song YS. 1999. Physiological functions of Chungkukjang. Food Ind Nutr 4: 40-46.
18 Lee JO, Ha SD, Kim AJ, Yuh CS, Bang IS, Park SH. 2005. Industrial application and physiological function of Chungkukjang. Food Sci Ind 38: 69-78.
19 Lee KA, Jang JO, Yoon HK, Kim MS. 2007. Antithrombotic activities of Cheongkookjang and Cheongkookjang fermented with green tea or mugwort. Korean J Microbiol 43: 298-303.   과학기술학회마을
20 Watanabe S, Haba R, Terashima K, Arai Y, Miura T, Chiba D, Takamatsu M. 2000. Antioxidant activity of soya hypocotyl tea in humans. Biofactors 12: 227-232.   DOI
21 Verdrengh M, Jonsson IM, Holmdahl R, Tarkowski A. 2003. Genistein as an anti-inflammatory agent. Inflamm Res 52: 341-346.   DOI
22 Seo HR, Kim JY, Bae GH, Park KY. 2009. Antiproliferative effect of Bacillus subtilis fermented soy milk in AGS human gastric adenocarcinoma cells. J Korean Soc Food Sci Nutr 38: 644-648.   과학기술학회마을   DOI
23 Shon DH. 1997. Nutritional and bioactive components of soymilk and cow's milk (a review). Korea Soybean Digest 14: 66-76.
24 Lee JE, Lee SY. 2001. Growth characteristics of Bifidobacteria and quality characteristics of soy yogurt prepared with different proteolytic enzymes and starter culture. Korean J Food Sci Technol 33: 603-610.   과학기술학회마을
25 Seo HR, Kim JY, Kim JH, Park KY. 2009. Identification of Bacillus cereus in a Chungkukjang that showed high anticancer effects against AGS human gastric adenocarcinoma cells J Med Food 12: 1274-1280.   DOI
26 Lew EA. 1985. Mortality and weight: insured lives and the American Cancer Society studies. Ann Intern Med 103:1024-1029.   DOI
27 Kopelman PG. 1994. Causes and consequences of obesity. Med Int 22: 385-388.