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http://dx.doi.org/10.3746/jkfn.2010.39.8.1119

Resveratrol Induces Apoptosis in Primary Human Prostate Cancer Cells  

Kang, Hye-In (Dept. of Food and Nutrition, Sunchon National University)
Kim, Jae-Yong (Research Institute of Basic Science, Sunchon National University)
Cho, Hyun-Dong (Dept. of Food and Nutrition, Sunchon National University)
Park, Kyung-Wuk (S-biofood)
Kang, Jum-Soon (Dept. of Hortcultural Bioscience, Pusan National University)
Seo, Kwon-Il (Dept. of Food and Nutrition, Sunchon National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.8, 2010 , pp. 1119-1125 More about this Journal
Abstract
To evaluate resveratrol as a prostate cancer preventive material, we investigated its anti-proliferative and apoptotic effects in RC-58T/h/SA#4 primary human prostate cancer cells. Resveratrol significantly decreased the number of viable RC-58T/h/SA#4 cells in a dose- and time-dependent manner. Resveratrol showed cytotoxicity against RC-58T/h/SA#4, LNCaP, PC-3 human prostate cancer cells with $IC_{50}$ values of 245, 320 and $340\;{\mu}M$, respectively. However the cytotoxic potential of resveratrol against normal RWPE-1 cells was lower ($IC_{50}=982\;{\mu}M$). Resveratrol induced cell death as evidenced by the increased formation of apoptotic bodies, nuclear condensation, sub-G1 phase, and DNA fragmentation. Resveratrol activated initiator caspases 8, and 9 as well as effector caspase 3 in a dose-dependent manner. Furthermore, the general caspase inhibitor z-VAD-fmk significantly inhibited resveratrol-induced apoptosis compared to cells without treatment. These results clearly indicate that resveratrol-induced apoptosis was dependent on caspase activation. Further, resveratrol modulated the down regulation of Bcl-2 (anti-apoptotic), and Bid. However, the level of Bax (pro-apoptotic) remained unchanged. These results suggest that resveratrol induced apoptosis in RC-58T/h/SA#4 cells via a mitochondrial-mediated caspase-dependent pathway, suggesting therapeutic potential against prostate cancer.
Keywords
resveratrol; prostate cancer cell; RC-58T/h/SA#4; apoptosis;
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Times Cited By KSCI : 8  (Citation Analysis)
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