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http://dx.doi.org/10.3746/jkfn.2010.39.6.927

Defatted Grape Seed Extracts Suppress Adipogenesis in 3T3-L1 Preadipocytes  

Cho, Young-Min (Dept. of Food Science and Technology, Chungbuk National University)
Lee, Seon-Mi (Dept. of Food Science and Technology, Chungbuk National University)
Kim, Young-Hwa (Dept. of Food Science and Technology, Chungbuk National University)
Jeon, Geon-Uk (Dept. of Food Science and Technology, Chungbuk National University)
Sung, Jee-Hy (Dept. of Food Science and Technology, Chungbuk National University)
Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.6, 2010 , pp. 927-931 More about this Journal
Abstract
The objective of this study was to evaluate the effect of defatted grape seed extract (DGSE) on adipocyte differentiation in 3T3-L1 preadipocytes. DGSE at 100 ${\mu}g$/mL significantly suppressed lipid accumulation and glycerol-3-phosphate dehydrogenase activity in hormonally stimulated adipocytes, an indicator of adipocyte differentiation. In order to understand the anti-adipogenic effects of DGSE, the changes in the expression of several adipogenic transcription factors including peroxisome proliferator-activated receptor (PPAR) $\gamma$, CCAAT/enhancer-binding protein (C/EBP) $\alpha$ and $\beta$ were investigated using immunoblotting. DGSE suppressed the expression of PPAR$\gamma$, C/EBP$\alpha$, and C/EBP$\beta$ proteins compared with control adipocytes in a dose-dependent manner. This results indicated that DGSE may alter fat mass by directly affecting adipogensis in maturing preadipocytes and thus may have applications for the treatment of obesity.
Keywords
defatted grape seed extract; 3T3-L1; adipogenesis; PPAR$\gamma$; C/EBP;
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