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http://dx.doi.org/10.3746/jkfn.2010.39.2.219

Effects of Scutellaria baicalensis Water Extract on Lipid Metabolism and Antioxidant Defense System in Rats Fed High Fat Diet  

Yoon, Hye-Jin (Dept. of Food and Nutrition, Daegu University)
Park, Young-Sook (Dept. of Food and Nutrition, Daegu University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.2, 2010 , pp. 219-226 More about this Journal
Abstract
The objective of this study was to determine the effects of a Scutellaria baicalensis water extract (SDWE) on lipid levels, lipid peroxidation and antioxidant enzyme activities on rats fed a high fat diet for 6 weeks. Thirty-two male Sprague-Dawley rats (4-weeks-old) were randomly divided into four groups: normal diet and deionized water (ND), normal diet and Scutellaria baicalensis water extract (NDS), high fat diet and deionized water (HFD), high fat diet and Scutellaria baicalensis water extract (HFDS). The food intakes were significantly lower, but the food efficiency ratios were significantly higher in the high fat diet groups than those in other groups. The level of HDL-cholesterol and HDL-cholesterol/total cholesterol ratio in plasma were significantly higher and AI (atherogenic index) in HFDS group was significantly lower than that in HFD group. The level of triglyceride in plasma was significantly decreased in SDWE groups. The triglyceride of liver was significantly increased in the high fat diet groups and the total cholesterol of liver in the HFDS group was significantly lower than that in the HFD group. The plasma and liver concentrations of thiobarbituric acid reactive substances (TBARS) in the NDS group were significantly lower than those in the ND group. The total antioxidant status (TAS) in plasma was significantly increased in the HFDS group compared to the HFD group. The activities of SOD, catalase and GST were significantly increased in SDWE groups compared to ionized water groups. The activity of GSH-Px and the concentration of GSH in liver in the HFDS group were significantly higher than those in the HFD group. These results suggest that a supplement of SDWE on rats fed high fat diet reduce levels of lipid and lipid peroxidation in plasma and liver and improve the antioxidant defense systems.
Keywords
Scutellaria baicalensis; lipid peroxidation; antioxidant defense system;
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Times Cited By SCOPUS : 4
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1 Gao D, Tawa R, Masaki H, Okano Y, Sakurai H. 1998. Protective effects of baicalein against reactive oxygen species. Chem Pharm Bull 46: 1383-1387.   DOI
2 Kimura Y, Kubo M, Tani T, Arichi OH. 1981. Studies on Scutellariae Radix: Effects on lipid peroxidation in rat liver. Chem Pharm Bull 29: 2610-2617.   DOI
3 Hamada H, Hiramatsu M, Edamatsu R, Mori A. 1993. Free radical scavenging action of baicalein. Arch Biochem Biophys 306: 261-266.   DOI
4 Yoshino M, Murakami K. 1998. Interaction of iron with polyphenolic compounds: application to antioxidant characterization. Anal Biochem 257: 40-44.   DOI
5 American Institute of Nutrition. 1976. Report of the American institute of nutrition Ad Hoc Committee on standards for nutritional studies. J Nutr 107: 1340-1348.
6 Chung CK, Han SS, Lee SY, Oh DH, Choi SY, Kang IJ, Nam SM. 1999. Effects of Houttyunia cordata ethanol extracts on serum lipids and antioxidant enzymes in rats fed high fat diet. J Korean Soc Food Sci Nutr 28: 205-211.   과학기술학회마을
7 Muller PH. 1977. A fully enzymatic triglyceride determination. J Clin Chem Clin Biochem 15: 457-464.
8 Richmond W. 1976. Use of cholesterol oxidase for assay of total and free cholesterol in serum continuous flow analysis. Clin Chem 22: 1579-1588.
9 Finley PR, Schifman RB, Williams RJ, Luchit DA. 1978. Cholesterol in high-density lipoprotein. Clin Chem 24: 931-933.
10 Friedewald WT, Levy RI, Fredrickson DS. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499-506.
11 Byun JH. 1995. The goals and strategy for health promotion. Korean Institute for Health and Social Affairs.
12 Yagi K. 1994. Lipid peroxides and related radicals in clinical medicine In Free Radicals in Diagnostic Medicine. Armstrong D, ed. Plenum press, New York, USA. p 1-15.
13 Arthur JR, Boyne R. 1985. Superoxide dismutase and glutathione peroxidase activities in neutrophils from selenium deficient and copper deficient cattle. Life Sci 36: 1569-1575.   DOI
14 Aebi H. 1974. Catalase in vitro. Meth Enzymol 105: 121-126.
15 Paglia ED, Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70: 158-169.
16 Habig WH, Pabst MJ, Jakoby WB. 1974. Glutathione S-transferase: The first enzymatic step in mercapture acid reaction. Anal Biochem 249: 7130-7139.
17 Ellman GL. 1959. Tissue sulfhydryl group. Arch Biochem Biophys 82: 70-72.   DOI
18 Lee JS, Lee KH, Jeong JH. 1999. Effects of extract of Pueraria radix on lipid metabolism in rats fed high fat diet. J Korean Soc Food Sci Nutr 28: 218-224.
19 Sung TS, Son GM, Bae MJ, Choi C. 1992. Effect of Acanthopanacis Cortex boiling extract solutions on fat accumulation in the obese rats induced by high fat dietary. J Korean Soc Food Nutr 21: 9-16.   과학기술학회마을
20 Sohn YS. 2003. Studies on hyperlipidemia in Korean (1)－ hyperlipidemia in normal and hypertensive person. J Korean Med Sci 18: 354-360.
21 Kang YH, Park YK, Ha TY, Moon KD. 1996. Effects of pine needle extract on enzyme activities of serum and liver and liver morphology in rats fed high fat diet. J Korean Soc Food Nutr 25: 374-378.
22 Folch J, Lees M, Sloanestanley GH. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem 226: 497-509.
23 Kim HJ, HwangBo MH, Lee JW, Im HG, Lee IS. 2007. Antioxidant effects of ginseng powder on liver of benzo ($\alpha$)pyrene-treated mice. Korean J Food Sci Technol 39: 217-221.   과학기술학회마을
24 Lovlin R, Cottle W, Pyke I, Kavanagh M, Belcastro AN. 1987. Are induces of free radical damage related to exercise intensity. Eur J Appl Physiol Occup Physiol 6: 313-316.
25 Rivero A, Monreal JI, Gil MJ. 1994. Peroxisome enzyme modification and oxidative stress in rats by hypolipidemic and antinflammatory drugs. Res Esp Fisiol 50: 259-268.
26 Ciriolo MR, Rossi L, Mavelli I, Rotilio G, Borzatta V, Cristofori M, Barbanti M. 1984. The effects of hypolipidemic agents derived from procetofenic acid on the activity of superoxide dismutase and glutathione peroxidase and on malonydialdehyde production of rat liver. Arzneimittelforschung 34: 456-457.
27 Choi HS, Kim YH, Han JH, Park SH. 2008. Effect of Eleutherococcus senticoccus and several oriental medicinal herbs extracts on serum lipid concentrations. Korean J Food Nutr 21: 210-217.
28 Bochorakova H, Paulava H, Slanina J, Musil P, Taborska E. 2003. Main flavonoids in the root of Scutellaria baicalensis cultivated in Europe and their comparative antiradical properties. Phytother Res 17: 640-644.   DOI
29 Chi TS, Lim H, Park H, Kim HP. 2003. Effects of wogonin, a plant flavone from Scutellaria radix on skin inflammation: in vivo regulation of inflammation-associated gene expression. Biochem Pharmacol 66: 1271-1278.   DOI
30 Huang Y, Tsang SY, Tao X, Lau CW, Su YL, Chen ZY. 2002. Baicalin induced vascular response in rat mesenteric artery: role of endothelial nitric oxide. Clin Exp Pharmacol 29: 721-724.   DOI
31 Igarashi K, Ohmuma M. 1995. Effect of isorhamnetin, rhamnetin and quercitin on the concentration of cholesterol and lipoperoxide in the serum and liver and on the blood and liver antioxidative enzyme activities of rats. Biosci Biotech Biochem 59: 595-598.   DOI
32 Kim YH. 2005. Scutellaria baicalensis extract inhibit development of atopic dermatitis in NC/Nga mouse. MS Thesis. Daegu University, Gyeongsan, Korea. p 21.
33 Song YO, Chyun JH. 2004. Effect of β-carotene supplementation on lipid peroxides and antioxidative enzyme activities in hyperlipidemic rats. Korean J Nutr 37: 771-779.   과학기술학회마을
34 Nam JH, Park HS. 1993. Effect of quality and quantity of dietary fat on status of tocopherol and lipid peroxidation of plasma tissue in rats. Korean J Nutr 26: 566-577.
35 Kim SC, Ahn KS, Park CK, Jeon BS, Lee JT, Park WJ. 2006. Isolation of antioxidative compound from Scutellaria baicalensis G. Korean J Med Crop Sci 14: 212-216.   과학기술학회마을
36 Shin SH, Kim MK. 2004. Effect of dried powders or ethanol extracts of garlic flesh and peel on antioxidative capacity in 16-month-old rats. Korean J Nutr 37: 633-644.   과학기술학회마을
37 Halliwell B, Gutteridge JMC. 1999. Free radicals in biology and medicine. 3rd ed. Oxford University Press, New York, USA.
38 Yuling Z, Hailing L, Zhonghong G, Yuefa G, Huibi X. 2006. Effect of flavonoids extracted from Scutellaria baicalensis Georgi on hemin-nitrite-$H_2O_2$ induced liver injury. Euro J Pharmacol 536: 192-199.   DOI   ScienceOn
39 Nam SM, Ham SS, Oh DH, Jung ME, Kang IJ, Chung CK. 2002. Effects of Eucommia ulmoides Olivon ethanol extract on lipid metabolism and antioxidant enzyme activities of rats fed high fat diet. J Korean Soc Food Sci Nutr 31: 796-801.   과학기술학회마을   DOI
40 Hatano T. 1995. Constituents of natural medicines with scavenging effects on active oxygen species-tannins and related polyphenols. Natural Med 49: 357-363.