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http://dx.doi.org/10.3746/jkfn.2010.39.2.203

Effects of Petasites japonicus and Momordica charantia L. Extracts on MC3T3-E1 Osteoblastic Cells  

Ji, Suk-Hee (Dept. of Food Science and Nutrition, Dong-A University)
Ahn, Do-Hwan (Dept. of Physiology, Kosin Medical College)
Jun, Mi-Ra (Dept. of Food Science and Nutrition, Dong-A University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.2, 2010 , pp. 203-209 More about this Journal
Abstract
In this study, the effects of Petasites japonicus and Momordica charantia L. extracts on MC3T3-ET1 osteoblastic cells were investigated. Since the activity of osteoblastic cell is one of the important factors for bone formation, the cellular proliferation of osteoblast was evaluated by MTT and alkaline phosphatase (ALP) activity. Compared to control, the cell proliferation was elevated to 114% and 112% by the treatment of Petasites japonicus and Momordica charantia L. extracts, respectively at the concentration of $10\;{\mu}g/mL$. The cell differentiation was also measured by alkaline phosphatase (ALP) activity at 3, 7, 14, and 27 days treatments with one of the extracts, respectively. As results, the ALP activity was significantly increased at 3 days, compared to control (p<0.05). To evaluate the effect of Petasites japonicus and Momordica charantia L. extracts on bone nodule formation, MC3T3-E1 cells were cultured in $\alpha$-MEM for 3, 14, and 21 days and then stained by alizarin red. To determine the expression patterns of bone-related proteins during the MC3T3-E1 osteoblast-like cell differentiation, osteoblast cells were cultured in $\alpha$-MEM for 24 hr. RNA was extracted and RT-PCR analysis was performed to examine the expression of OPG, RANKL and osteocalcin. Petasites japonicus extract exhibited the significant increment of osteocalcin compared with the positive control, which suggests that Petasites japonicus may have beneficial effects on bone health through the proliferation of osteoblast cells.
Keywords
Petasites japonicus; MC3T3-E1; OPG; RANKL; alkaline phosphatase (ALP);
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1 Teitelbaum SL, Ross FP. 2003. Genetic regulation of osteoclasts development and function. Nat Rev Genet 4: 638-649.   DOI
2 Suzuki H, Hayakawa M, Kobayashi K, Takiguchi H, Abiko Y. 1997. H2O2-derived free radicals treated fibronectin substratum reduced the bone nodule formation of rat calvarial osteoblast. Mech Aging Dev 98: 113-125.   DOI
3 Hall TJ, Schaeublin M, Jeker H, Fuller K, Chambers TJ. 1995. The role of reactive oxygen intermediates in osteoclasic bone resorption. Biochem Biophys Res Commun 207: 280-287.   DOI
4 Datta HK, Rathod H, McNeil CJ. 1996. Parathyroid hormone induces superoxide anion burst in the osteoclast by the hormone. Endocrinology 149: 269-275.   DOI
5 Anderson JJ, Graner SC. 1998. Phytoestrogen and bone. Bailliere Clin Endoc 12: 543-557.   DOI
6 Kim MR, Yang CH, Seo BI. 1998. Effects of safflower seeds on bone mineral density in ovarientomy-induced postmenopausal osteoporotic rats. Korean J Herbo 13: 37-43.
7 Setchell KDR, Cassidy A. 1999. Dietary isoflavones: biological effects and relevance to human health. J Nutr 129: 758S-767S.   DOI
8 Lee HK. 1998. Effect of black bean and samryungbackchulsan on ovariectomy-induced postmenopausal osteoporotic rats. PhD Dissertation. Kyung Hee University, Seoul, Korea.
9 Ha H, Kwak HB, Lee SW, Jin HM, Kim HM, Kim HH, Lee ZH. 2004. Reactive oxygen species mediate RANK signaling in osteoclasts. Exp Cell Res 301: 119-127.   DOI
10 Reddy SV. 2004. Regulatory mechanisms operative in osteoclasts. Crit Rev Eukaryot Gene Expr 14: 255-270.   DOI
11 Choi OB. 2002. Anti-allergic effects of Petasites japonicus. Korean J Food Nutr 15: 382-385.   과학기술학회마을
12 Oh SI, Kim GH, Kwak CS, Lee MS. 2001. Effect of melatonin on the antioxidative enzyme activities in type 1 osteoporosis. Korea J Gerontol 11: 29-35.
13 Park Y, Park YL, Cho DH, Lee HH. 2007. Antioxidant activity of Momordica charantia L. extracts. Korean J Medicinal Crop Sci 15: 56-61.   과학기술학회마을
14 Suda T, Takagashi N, Udagawa N, Jimi E, Gillespie MT. 1999. Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr Rev 20: 345-357.   DOI
15 Duda RJ, O'Brien JF, Katzmann JA. 1988. Concurrent assays of circulating bone-gla protein and alkaline phophatase: effects of sex, age, and metabolic bone disease. J Clin Endocr Meta 66: 951-957.   DOI
16 Pacifici R. 1996. Estrogen, cytokines and pathogenesis of postmenopausal osteoporosis. J Bone Mine Res 11: 1043-1051.   DOI
17 Ha WH, Hwang DS. 2007. Identification of receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL) and osteoprotegerin (OPG) in ameloblastoma. Oral Maxillofac J Kor Surg 33: 94-102.   과학기술학회마을
18 Kawamoto S, Ejiri S, Hoshi K. 2002. Immunolocalization of osteoclast differentiation factor in rat periodontium. Arch Oral Biol 47: 55-58.   DOI
19 Grover JK, Yadav SP. 2000. Pharmacological actions and potential uses of Momordica charantia: a review. J Ethnopharmacol 93: 123-132.
20 Yasuda H, Shima N, Nakagawa N. 1998. Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis is a ligand for osteoprotegerin-inhibitory factor and is identical to TRANCE/RANKL. Proc Natl Acad Sci 95: 3597-3602.   DOI
21 Hofbauer LC, Khosla S, Dunstan CR, Lacey DL, Boyle WJ, Riggs BL. 2000. The roles of osteoprotegerin ligand in the paracrine regulation of bone resorption. J Bone Miner Res 15: 2-12.
22 Suda T, Takahashi N, Udagawa N, Jimi E, Gillespie MT, Martin TJ. 1999. Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocrine Rev 20: 345-357.   DOI
23 Cho SH. 1995. The electron microscopic observation of the effects of estrogen on the osteoblast-like cell differentiation. Korean Soc Menopause 1: 28-34.
24 Lee J, Lee I. 2004. Effect of Rubus coreanus M. extract on the activity and differentiation of MC3T3-E1 osteoblastic cell. J Life Sci 14: 967-974.   DOI
25 Lee YS. 2001. Effect of isoflavones on proliferaion and oxidative stress of MC3T3-E1 osteoblast-like cells. Korea Soybean Digest 18: 35-42.
26 Park JY. 2007. The effect of Petasites japonicus extract on hepatotoxicity in rats. Kor J Env Hlth 33: 202-206.   과학기술학회마을   DOI
27 Jee YH, Lee CS. 1996. Pathological changes on rats and mice fed with Petasites japonicus Maxim. Korean J Vet Res 36: 417-428.
28 Oh SH, Yang YH, Kwon OY, Kim MR. 2006. Effects of diet with added butterbur (Petasites japonicus Maxim) of the plasma lipid profiles and antioxidant index of mice. J East Asian Soc Dietary Life 16: 399-407.   과학기술학회마을
29 Wasnich R. 1996. What is an osteoporotic fracture. In Osteoporosis: Diagnostic and Therapeutic Principles. Rosen CJ, ed. Humana Press, NJ, USA. p 79-88.
30 Pittenger JJ, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD. 1999. Multilineage potential of adult human mesenchymal stem cells. Science 284: 143-147.   DOI
31 Lee C, Jang Y, Park J, Yoon S. 2007. Antioxidant and cell proliferation effects of Acanthopanax senticosus extract in human osteoblast-like MG-63 cell line. Korean J Food Sci Technol 39: 694-700.   과학기술학회마을
32 Jang H, Eom H, Roh S, Yun I. 2005. Effect of extracts from safflower seeds on osteoblastic differentiation and intracellular free calcium concentration in MC3T3-E1 cells. Korean J Physiol Pharmacol 9: 55-62.   과학기술학회마을
33 Matsunuma M, Kawane T, Horiuchi N. 2001. Simvastatin promotes osteoblast differentiation and mineralization in MC3T3-ET1 cell. Biochem Biophy Res Comm 208: 874-877.
34 Schiller PC, D'ipplito G, Balkan W, Roos BA, Howard GA. 2001. Gap-junctional communication is required for the maturation process of osteoblastic cell in culture. Bone 28: 362-369.   DOI