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http://dx.doi.org/10.3746/jkfn.2010.39.12.1769

Effect of Ecklonia stolonifera Extracts on Bone Turnover Markers in Ovariectomized Rats  

Kim, Young-Kyoung (Dept. of Food and Nutrition, Silla University)
Kim, Mi-Hyang (Dept. of Food and Nutrition, Silla University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.12, 2010 , pp. 1769-1775 More about this Journal
Abstract
Menopause is often associated with the incidence of several chronic diseases including osteoporosis, cardiovascular disease, and obesity. The purpose of this study was designed to evaluate the possibility of osteoporosis prevention in postmenopausal women. In this study, we investigated the effects of Ecklonia stolonifera (ES) extracts on bone turnover markers in ovariectomized rats. For this study, the following four groups of 9-week-old Sprague-Dawley rats were evaluated over 6 weeks: normal rats (SHAM), ovariectomized rats (OVX-CON) and ovariectomized rats that were treated with ES extracts. We measured the osteocalcin and C-telopeptide of collagen cross-links (CTx) content, enzyme ALP activity in serum and collagen content in the cartilage, bone, skin and lungs. We found that the levels of indicators of bone metabolism such as alkaline phosphatase (ALP), osteocalcin and CTx were lower in rats in the ES extract group than the OVX-CON group. In addition, the collagen contents in the bone, cartilage, skin and lungs decreased in response to ovariectomy, but the levels of collagen were greater in the bone of rats that were treated with ES extract than in the bone of rats in the OVX-CON group. These results suggest that the ES may be an effective functional food to prevent osteoporosis in postmenopausal women.
Keywords
Ecklonia stolonifera; ALP; osteocalcin; CTx; collagen; ovariectomized rats;
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1 Jang JS. 1987. Bone metabolism and its hormonal regulation. Current Medical Research and Opinion 30: 11-15.
2 Kim SJ. 2003. Study on the relationship between osteoporosis- cause factor and bone mineral density, Biochemical Marker. MS Thesis. Pukyong National University, Busan, Korea.
3 Park HM, Kim TC, Kim TC, Kang KH, Yoon SJ, Hur M.2000. The predictive value of changes of bone markers for changes of bone mineral density in postmenopausal hormone replacement therapy with or without active vitamin D. Korean J Obstetrics Gynecology 43: 268-274.
4 Bonde M, Garnero P, Fledelius C, Qvist P, Delmas PD, Christiansen C. 1997. Measurement of bone degradation products in serum using antibodies reactive with an isomerized from of an 8 amino acid sequence of the C-telopeptide of type Ⅰ collagen. J Bone Mine Res 12: 1028-1034.   DOI
5 Garnero P, Hausherr E, Chapuy MC, Marcelli C, Garndjean H, Muller C, Cormier C, Breart G, Meunier PJ, Delmas PD. 1996. Markers of bone resorption predict hip fracture in elderly women: the EPIDOS Prospective Study. J Bone Miner Res 11: 1531-1538.   DOI
6 Ryu BH, Chi BH, Kim DS, Ha MS. 1986. Desmutagenic effect of extracts obtained from seaweed. J Korean Fish Soc 19: 502-508.   과학기술학회마을
7 Woessner JF. 1961. The determination of hydroxyproline in tissue and protein sample containing small proportion of this amino acid. Arch Biochem Biophys 93: 440-447.   DOI
8 Abe T, Chow JM, Lean JM, Chambers TJ. 1993. Estrogen does not restore bone list after ovariectomy in the rat. J Bone Miner Res 8: 831-838.   DOI
9 Cho KJ, Lee YS, Ryu BH. 1990. Antitumor effect and immunology activity of seaweeds toward sarcoma-180. Bull Kor Fish Soc 23: 345-352.   과학기술학회마을
10 Kim SA, Kim J, Woo MK, Kwak CS, Lee MS. 2005. Antimutagenic and cytotoxic effects of ethanol extracts from five kinds of seaweeds. J Korean Soc Food Sci Nutr 34: 451-459.   과학기술학회마을   DOI
11 Kwon MJ, Nam TJ. 2006. Effects of Mesangi (Capsosiphon fulvecens) power on lipid metabolism in high cholesterol fed rats. J Korean Soc Food Sci Nutr 35: 530-535.   과학기술학회마을   DOI
12 Kang JW. 1966. On the geographical distribution of marine algae Korea. Bull Pusan Fish Coll 7: 1-125.
13 Kim OK, Lee TG, Park YB, Park DC, Lee YW, Yeo SG, Kim IS, Park YH, Kim SB. 1996. Inhibition of xanthine oxidase by seaweed extracts. J Korean Soc Food Sci Nutr 25: 1069-1073.   과학기술학회마을
14 Park YB. 2005. Determination of nitrite scavenging activity of seaweed. J Korean Soc Food Sci Nutr 34: 1293-1296.   과학기술학회마을   DOI
15 Kim JH, Lee DS, Lim CW, Park HY, Park JH. 2002. Antibacterial activity of sea-mustard, Laminaria japonica extracts on the cariogenic bacteria, Streptococcus mutans. J Korean Fish Soc 35: 191-195.   과학기술학회마을   DOI
16 Korea National Statistical Office. 2008. Lifetable. Seoul,Korea.
17 Clark AP, Schuttinga JA. 1992. Targeted estrogen/progesterone replacement therapy for osteoporosis: calculation of health care cost savings. Osteoporos Int 2: 195-200.   DOI
18 Nakamura YN, Iwamoto H, Ono Y, Nishimura S, Tabata S. 2003. Relationship among collagen amount, distribution and architecture in the M. Longissimus thoracis and M. pectoralis profundus from pigs. Meat Sci 64: 43-50.   DOI
19 Kim MH, Otsuka M, Arakawa N. 1994. Age-related change in the pyridinoline content of guinea pigs cartilage and achilles tendon collagen. J Nutr Sci Vitaminol 40: 95-103.   DOI   ScienceOn
20 Tiki ML, Allison GT. 2003. Malondialdehyde oxidation of cartilage collagen by chondrocyte. Osteo Arthritis Research Soc Int 11: 159-166.
21 Cecchettin M, Bellomatti S, Cremonesi G, Solomeno LP, Torrim G. 1995. Metabolic and bone effects after administration of ipriflavone and salmon calcitonin postmenopausal osteoporosis. Biomed Pharmacother 49: 465-468.   DOI
22 Pi YZ, Wu XP, Liu SP, Luo XH, Cao XZ, Xie H, Liao EY. 2006. Age-related changes in bone biochemical markers and their relationship with bone mineral density in normal Chinese woman. J Bone Miner Res 24: 380-385.   DOI
23 Kim IG, Kim SB, Kim JG, Kim KC. 1993. Serum enzymes as indicators of radiation exposure in rat. J Radiation Protection 16: 37-44.   과학기술학회마을
24 Khandwala HM, Mumm S, Whyte MP. 2006. Low serum alkaline phosphatase activity and pathologic fracture: case report and brief review of hypophosphatasia diagnosed in adulthood. Endocr Pract 12: 676-681.   DOI
25 Valette A, Meignen KM, Mercier L, Liehr JG, Boyer J. 1986. Effect of 2-fluoroestradiol on lipid metabolism in the ovariectomized rat. J Steroid Biochem 25: 575-578.   DOI
26 Aitken JM, Armstrong E, Anderson JB. 1972. Osteoporosis after oophorectomy in the mature female rat and the effect of oestrogen and/or progesterone replacement therapy in its prevention. J Endocrinol 55: 79-87.   DOI
27 Wronski TJ, Cintron M, Dann LM. 1988. Temporal relationship between bone loss and increased bone turnover ovariectomized rats. Calcif Tissue Int 43: 179-183.   DOI
28 Ramirez ME, McMurry MP, Wiebke GA, Felton KJ, Ren K. 1997. Evidence for sex steroid inhibition of lipoprotein lipase in men; comparison of abdominal and femoral adipose tissue. Metabolism 46: 179-185.   DOI
29 Turner RT, Wakley GK, Hannon KS. 1974. Tamoxifen prevents the skeletal effects of ovarian hormone deficiency in rats. J Bone Mineral Res 2: 449-459.   DOI
30 Muller K, Hsiao S. 1980. Estrus- and ovariectomy-induced body weight changes: evidence for two estrogenic mechanisms. J Comp Physiol Psychol 94: 1126-1134.   DOI
31 Mook DG, Kenney NJ, Roberts S, Nussbaum AI, Rodier WI. 1972. Ovarian-adrenal interactions in regulation of body weight by female rats. J Comp Physiol Psychol 81: 198-211.   DOI
32 Park JC, Hur JM, Gwon HJ, Kim HJ, Chun SS, Choi JS, Choi JW. 2000. Effects of phloroglucinol isolated from Ecklonia stolonifera on the acetaminophen-metabolizing enzyme system in rat. J Korean Soc Food Sci Nutr 29: 448-452.   과학기술학회마을
33 Sung JH, Ha S, Im MH, Im JG, Kang KS. 2002. Seaweed and health. In Foods and Health. Hyungseol Press Co, Seoul, Korea. p 190.
34 Kimble RB, Vannice JL, Bloedowl DC. 1994. Interleukin-1 receptor antagonist decreases bone loss and bone resorption in ovariectomized rats. J Clin Invest 93: 1959-1967.   DOI
35 Lee KE, Park YJ, Byun SJ, Yoo EK, Lee ML, Lee YS, Lee HK, Chung ES, Cho OS, Choi ES, Han HS. 1997. Women Health Nursing. Hyunmoonsa, Seoul, Korea.
36 Johnston CC, Hui SI, Witt RM, Appledorn R, Baker S, Longcope C. 1985. Early menopausal changes in bone mass and sex steroids. J Clin Endocrinol Metab 61: 905-911.   DOI
37 Richelson LS, Wahner HW, Melton LJ. 1984. Relative contributions of aging and estrogen deficiency to postmenopausal bone loss. New Engl J Med 37: 1273-1278.
38 Lee YA, Kim M. 2008. Effects of sea tangle extract on formation of collagen and collagen cross-link in ovariectomized rats. J Life Sci 18: 1578-1583.   과학기술학회마을   DOI
39 Kimble RB, Matayoshi AB, Vannice JL. 1995. Simultaneous block of interleukin-1 and tumor necrosis factor is required to completely prevent bone loss in the early postovariectomy period. Endocrinology 136: 3054-3061.   DOI
40 Dempster DW, Birchman R, Xu R. 1995. Temporal changes in cancellous bone structure of rats immediately after ovariectomy. Bone 16: 157-161.
41 Lee HS, Jin SH, Kim HS, Ryu BH. 1995. Characteristic properties of fucoidan sulfate purified from Gompi, Ecklonia stolonifera. Korean J Food Sci Technol 27: 716-723.   과학기술학회마을