Browse > Article
http://dx.doi.org/10.3746/jkfn.2010.39.10.1433

The Antioxidative and Antimicrobial Effects of Internal Organs of Aplysia kurodai Fractions  

Shin, Mi-Ok (Dept. of Food and Nutrition, Silla University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.10, 2010 , pp. 1433-1438 More about this Journal
Abstract
This study was carried out to investigate the antioxidative and antimicrobial activities of internal organs of Aplysia kurodai (AK). The internal organs of AK were extracted with methanol (AKM), which was then further fractionated into four subfractions by using solvent partition method, affording hexane (AKMH), methanol (AKMM), butanol (AKMB), and aqueous (AKMA) soluble fractions. The antioxidative activity of fractions from AK was investigated by measuring the scavenging activities of AK against DPPH radical, peroxynitrite ($ONOO^-$) and reactive oxygen species (ROS). Among the various solvent fractions, AKMB showed a marked scavenging effect against DPPH radical, peroxynitrite ($ONOO^-$) and reactive oxygen species (ROS). The antimicrobial activity was increased in proportion to its concentration by the paper disk method. Among the various solvent fractions, AKMM fractions of AK showed the strongest antimicrobial activities. The methanol extracts exhibited antimicrobial activity against all organisms tested, while the hexane extracts showed antimicrobial activity only against Proteus vulgaris. The results suggest that the AK may be suitable for development as a food preservative and alternative antioxidant.
Keywords
antioxidative activity; antimicrobial activity; internal organs of Aplysia kurodai (AK);
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Harman D. 1956. Aging: a theory based on free radical and radiation chemistry. J Gerontol 2: 298-300.
2 Shin MO, Bae SJ. 2009. The anticarcinogenic and antioxidative activity of Hemicentrotus pulacherrimus fractions in various cancer cells. J Life Sci 19: 607-614.   DOI
3 Kang DY, Shin MO, Shon JH, Bae SJ. 2009. The antioxidative and antimicrobial effects of Celastrus orbiculatus. J Life Sci 19: 52-57.   DOI
4 Carr AC. 2000. Oxidation of LDL by myeloperoxidase and reactive nitrogen species: reaction pathways and antioxidant protection. Arterioscler Thromb Vasc Biol 20: 1716-1723.   DOI   ScienceOn
5 Chung HY, Kim HJ, Kim KW, Chio JS, Yu BP. 2002. Molecular inflammation hypothesis of aging based on the anti-aging mechanism of calorie restriction. Micro Res Techinq 59: 264-272.   DOI
6 Pannala AS, Rice-Evans CA, Halliwell B, Singh S. 1997. Inhibition of peroxynitrite-mediated tyrosine nitration by catechin polyphenols. Biochem Biophy Res Commu 232: 164-170.   DOI
7 Blois MS. 1958. Antioxidant determination by the use a stable free radical. Nature 181: 1199-1200.   DOI
8 Paraiathathu T, Kegrer JP. 1992. Production of reactive oxygen by mitochondria from normoxic and hypoxic rat heart tissue. Free Radic Biol Med 13: 289-297.   DOI
9 Crow JP. 1997. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide 1: 145-157.   DOI
10 Davidson PM, Parish ME. 1989. Methods for testing the efficacy of food antimicrobials. J Food Sci Technol 1: 148-155.
11 Lee SH, Shin JH, Koo MO, Jung ES, Jeon GI, Park EJ, Park HR, Lee SC. 2007. Antigenotoxic activities of extracts from anglerfish. J Korean Soc Food Sci Nutr 36: 1229-1234.   DOI
12 Jeong CH, Bae YI, Shim KH, Choi JS. 2004. DPPH radical scavenging effect and antimicrobial activity of plantain (Plantago asiatica L.) extracts. J Korean Soc Food Sci Nutr 33: 1601-1605.   DOI
13 Bae SJ. 2004. Studies on the antioxidative and antimicrobial effects of Chondria crassicaulis. J Life Sci 14: 411-416.   DOI
14 Jung YH, Jung BM, Kang DY, Ku MJ, Shin MO, Bae SJ. 2006. The antioxidative and antimicrobial effects of Gloiopeltis tenax. Korean J Nutr 39: 366-371.
15 Lee HJ, Kim YA, Ahn JW, Lee BJ, Moon SG, Seo YW. 2004. Screening of peroxynitrite and DPPH radical scavenging activities from salt marsh plants. Korean J Biotechnol Bioeng 19: 57-61.
16 Lee HJ, Lee BJ, Lee DS, Seo TW. 2003. DPPH radical scavenging effect and in vitro lipid peroxidation inhibition by Potulaca oleracea. Korean J Biotechnol Bioeng 18: 165-169.
17 Ames BN, Shigenoga MK, Hagen TM. 1993. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci 90: 7915-7922.   DOI
18 Lee JS, Min DK. 2002. A catalogue of molluscan fauna in Korea. Opisthobranchs 18: 93-97.
19 Nishiwaki S, Ueda H, Makioka T. 1975. Tagging studies on the growth of the sea hare Aplysia kurodai on an intertidal rocky shore. Marine Biology 32: 389-395.   DOI
20 Tusa Y. 1994. Size-related egg production in a simultaneous hermaphrodite, the sea hare Aplysia kurodai baba (mollusca: opisthobranchia). Publ Seto Mar Biol 36: 249-254.   DOI
21 Kim CH, Seo HJ, Hwang EY, Kim EJ, Go HJ, Kim IH, Seo JK, Moon JH, Huh MD, Park NG. 2001. Purification of myomodulin A and myomodulin E from the central nervous system of the sea lare, Aplysia kurodai. J Korean Fish Soc 34: 279-284.
22 Kim JH, Ha JH, Lee EH. 1997. Refining of squid viscera oil. J Kor Soc Appl Biol Chem 40: 294-300.
23 Lloyd PE, Kuufermann I, Weiss KR. 1987. Sequence of small cardioactive peptide A: A second member of a class of neuropeptides in Aplysia. Peptides 8: 179-184.   DOI
24 Morris HR, Panico M, Karplus A, Lloyd PE, Riniker B. 1982. Elucidation by FAB-MS of the structure of a new cardioactive peptide from Aplysia. Nature 300: 643-645.   DOI
25 Seo JH, Jeong YJ, Lee GD, Lee MH. 1999. Monitoring characteristics of protease isolated from squid viscera. J East Asian Soc Dietary Life 9: 195-199.
26 Jeong YJ, Seo JH. 2001. Quality characteristics for doenjang using squid internal organs. Korean J Food Sci Technol 33: 89-93.
27 Oh SC, Cho JS. 2002. The change of non-volatile organic acids in low salt fermented squid affected by adding to squid ink. J Korean Oil Chem Soc 20: 64-71.
28 Shim JH, Kim KM, Bae DH. 2003. Comparisons of physicochemical and sensory properties in noodles containing spinach juice, beetroot juice and cuttlefish ink. Food Eng Prog 7: 37-43.
29 Schneider JE, Price S, Maidt L, Gutteridge JM, Floyd RA. 1990. Methylene blue plus light mediates 8-hydroxy-2'-deoxyguanosine formation in DNA preferentially over strand breakage. Nucleic Acids Res 18: 631-635.   DOI
30 Wright A, Bubb WA, Hawkins CL, Davies MJ. 2002. Singlet oxygen-mediated protein oxidation: evidence for the formation of reactive side chain peroxides on tyrosine residues. Photochem Photobiol 76: 35-46.   DOI
31 Wayner DD, Burton GW, Ingold KU, Barclay LR, Locke SJ. 1987. The relative contributions of vitamin E, urate, ascorbate and proteins to the total peroxyl radical-trapping antioxidant activity of human blood plasma. Biochim Biophys Acta 924: 408-419.   DOI
32 Yu HE, Leaniza MM, Bae YJ, Lee DH, Park JS, Kwak HS, Kim HK, Lee JS. 2005. Screening and extraction condition of antiaging bioactive substances from medicinal plants. J Korean Soc Food Sci Nutr 34: 1136-1142.   DOI
33 Namiki M. 1990. Antioxidants/antimutagens in food. Crit Rev Food Sci Nutr 29: 273-300.   DOI
34 Cho MH, Bae EK, Ha SD, Park JY. 2005. Application of natural antimicrobials to food industry. Food Sci Ins 38: 36-45.
35 Lee JH, Lee SR. 1994. Some physiological activity of phenolic substances in plant foods. Korean J Food Sci Technol 26: 317-323.
36 Park JC, Choi JW. 1996. Screening of marine natural products on inhibitory effect of the formation of lipid peroxidation. Kor J Pharmacogn 27: 117-122.
37 Choe BL, Lee JR. 1994. Opisthobranchs (mollusca: gastropoda) from Ullung and Dog-do islands, Korea. Korean J Zool 37: 352-376.
38 Kandel ER, Schwartz JH, Jessell TM. 2000. Principles of neural science. 4th ed. Mc Graw-Hill, New York, USA. p 180.
39 Kim HS, Choe BL. 1981. The fauna of marine invertebrate in Ulreung is and Dog-do is, special report. The Conservation of Nature and Natural Resources 19: 193-200.
40 Stratton SP, Liebler DC. 1997. Determination of singlet oxygen-specific versus radical-mediated lipid peroxidation in photosensitized oxidation of lipid bilayers: effect of beta-carotene and alpha-tocopherol. Biochemistry 36: 12911-12920.   DOI
41 Rice-Evans C, Burdon R. 1993. Free radical-lipid interactions and their pathological consequences. Prog Lipid Res 32: 71-110.   DOI