Browse > Article
http://dx.doi.org/10.3746/jkfn.2010.39.10.1425

Antibacterial and Antioxidant Activities of Solvent Extracts from Different Parts of Hagocho (Prunella vulgaris)  

Seo, Jong-Kwon (Dept. of Food Science, International University of Korea)
Kang, Min-Jung (Namhae Garlic Research Institute)
Shin, Jung-Hye (Namhae Garlic Research Institute)
Lee, Soo-Jung (Dept. of Food Science and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University)
Jeong, Hey-Gwang (Dept. of Toxicology, College of Pharmacy, Chungnam National University)
Sung, Nak-Ju (Dept. of Food Science and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University)
Chung, Young-Chul (Dept. of Food Science, International University of Korea)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.10, 2010 , pp. 1425-1432 More about this Journal
Abstract
This study was designed to investigate the effects of antioxidant and antibacterial activities of water, ethanol and methanol extracts from root, stem and flower stalk of Hagocho (Prunella vulgaris). The solvent extract yields from root were higher than those from stem and flower stalk, and water extract showed the highest extraction yield against ethanol and methanol extracts. The contents of total phenolic and flavonoid in ethanol extract were significantly higher in stem extract compared with those of root and flower stalk. In the case of water and methanol extracts, however, the contents were the highest in flower stalk. The electron donating ability and reducing power in all test groups were significantly increased in a dose-dependent manner, and antioxidant activities were the highest in methanol extract. In extracts from different parts of Hagocho, the antioxidant activity was the highest in flower stalk followed by stem and root. ABTS radical scavenging ability of water and methanol extracts was above 50% at $100\;{\mu}g/mL$ concentration. Antibacterial activities did not show significant differences depending on parts of Hagocho. However, antibacterial activity of ethanol extract was higher than those of other extracts.
Keywords
Hagocho (Prunella vulgaris); antibacterial activity; antioxidant activity;
Citations & Related Records
Times Cited By KSCI : 15  (Citation Analysis)
Times Cited By SCOPUS : 4
연도 인용수 순위
1 Gordon MH. 1990. The mechanism of antioxidant action in vitro. In Food antioxidants. Hudson BJF, ed. Elsevier Applied Science, London, UK. p 1-8.
2 Jeong CH, Shim KH. 2006. Chemical composition and antioxidative activities of Platycodon grandiflorum leaves and stems. J Korean Soc Food Sci Nutr 35: 511-515.   DOI
3 Kim YE, Yang JW, Lee CH, Kwon EK. 2009. ABTS radical scavenging and anti-tumor effects of Tricholoma matsutake Sing. (pine mushroom). J Korean Soc Food Sci Nutr 38: 555-560.   DOI
4 Kim YC, Cho CW, Rhee YK, Yoo KM, Rho JH. 2007. Antioxidant activity of ginseng extracts prepared by enzyme and heat treatment. J Korean Soc Food Sci Nutr 36: 1482-1485.   DOI
5 Choi YM, Kim MH, Shin JJ, Park JM, Lee JS. 2003. The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr 32: 723-727.   DOI
6 Lee OH, Jung SH, Son JY. 2004. Antimicrobial activity of clove extract by extraction solvents. J Korean Soc Food Sci Nutr 33: 494-499.   DOI
7 Park KN, Lee SH. 2003. Antimicrobial activity of pine needle extract and horseradish on the growth of Vibrio. J Korean Soc Food Sci Nutr 32: 185-190.   DOI
8 Lee HA, Nam ES, Park SI. 2003. Antimicrobial activity of Maesil (Prunus mume ) juice against selected pathogenic microorganisms. J Korean Soc Food Sci Nutr 16: 29-34.
9 Lim JW, Lee GB. 1999. Studies on the antimicrobial activities of Prunus mume. J East Asian Soc Dietary Life 9: 442-451.
10 Park JC, Choi JS, Choi JW. 1995. Effects of the fractions from leaves, fruits, stems and roots of Cudrania tricuspidata and flavonoids on lipid peroxidation. Kor J Pharmacogn 26: 377-384.
11 Kim MH, Lee NH, Lee MH, Kwon DJ, Choi UK. 2007. Antimicrobial of aqueous ethanol extracts of Perilla frutescens var. acuta leaf. Korean J Food Culture 22: 266-273.
12 Ju JC, Shin JH, Lee SJ, Cho HS, Sung NJ. 2006. Antioxidative activity of hot water extracts from medicinal plants. J Korean Soc Food Sci Nutr 35: 7-14.   DOI
13 Choi SY, Kim SY, Hur JM, Choi HG, Sung NJ. 2006. Antioxidant activity of solvent extracts from Sargassum thunbergii. J Korean Soc Food Sci Nutr 35: 139-144.   DOI
14 Ito M, Moriyama A, Mastsumoto Y, Takaki N, Fukomoto M. 1985. Inhibition of xanthine oxidase by flavonoids. Agric Biol Chem 49: 2173-2176.   DOI
15 Lee SJ, Song EJ, Lee SY, Kim KBWR, Kim SJ, Yoon SY, Lee CJ, Ahn DH. 2009. Antioxidant activity of leaf, stem and root extracts from Orostachys japonicus and their heat and pH stabilities. J Korean Soc Food Sci Nutr 38: 1571-1579.   DOI
16 Jang MJ, Woo MH, Kim YH, Jun DY, Rhee SJ. 2005. Effects of antioxidative, DPPH radical scavenging activity and antithrombogenic by the extract of Sancho (Zanthoxylum schinifolium). Korean J Nutr 38: 386-394.
17 Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J Food Technol 36: 333-338.
18 Kim JS, Kang SS, Lee KS, Chang SY, Won DH. 2000. Quantitative determination of ursolic acid from Prunellae Herb. Kor J Pharmacogn 31: 416-420.
19 Lee SG, Yu MH, Lee SP, Lee IS. 2008. Antioxidant activity and induction of apoptosis by methanol extracts from avocado. J Korean Soc Food Sci Nutr 37: 269-275.   DOI
20 Kim HJ, Jun BS, Kim SK, Cha JY, Cho YS. 2000. Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctorius L.). J Korean Soc Food Sci Nutr 29: 1127-1132.
21 Shimano T, Mizuno M, Okamoto H, Adachi I. 1956. Studies on triterpenoids. IX. On the new component of Prunella, ursolic acid. J Pharm Soc J pn 76: 974-975.   DOI
22 Lee SJ, Sung NJ, Jeong HG, Shin JH, Chung YC, Seo JK. 2008. Antioxidant activity of methanol extracts from Prunella vulgaris. J Korean Soc Food Sci Nutr 37: 1535-1541.   DOI
23 Gutfinger T. 1981. Polyphenols in olive oil. J Am Oil Chem Soc 58: 966-968.   DOI
24 Moreno MIN, Isla MI, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical scavenging activity of propolis from several region of Argentina. J Enthropharmacol 71: 109-114.   DOI   ScienceOn
25 Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200.   DOI
26 Oyaizu M. 1986. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. J apanese J Nutr 44: 307-315.   DOI
27 Re R, Pellegrini N, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTs radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237.   DOI
28 James GC, Sherman N. 1987. Chemotherapeutic agents in microbiology, a laboratory manual, chemical agent of control. 2nd ed. Prentice Hall, NJ, USA. p 247-254.
29 Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric Biol Chem 51: 1333-1338.   DOI
30 Kim DS, Ahn BW, Yeum DM, Lee DW, Kim ST, Park YH. 1987. Degradation of carcinogenic nitrosamine formation factor by natural food components. Bull Korean Fish Soc 20: 463-468.
31 Sandra J. 1963b. Phytochemical studies of Prunella vulgaris and Prunella grandiflora- I. Flavonoids and phenolicarboxylic acids. Dissertations Pharm 15: 483-489.
32 Dorosh NM, Domaratskaya OP. 1954. Phytochemical studies on plants of the Prunella vulgaris variety and of the type of the common meadow geranium. Nauch Studenschesk Obshchestva L'vov. Med Inst. p 64-67.
33 Kojima H, Ogura H. 1986. Triterpenoids from Prunella vulgaris. Phytochemistry 25: 729-733.   DOI
34 Wang ZJ, Zhao YY, Tu GZ, Hong SL, Chen YY. 1999. Studies on the chemical constituents from Prunella vulgaris. Acta Pharm Sin 34: 679-681.
35 Ko IJ, Yoo SJ, Lee EB. 1986a. Pharmacological studies on Prunellae Herba and Thesii Herba ( I ). On antiinflammatory activity. Kor J Pharmacog 17: 232-241.
36 Ko IJ, Yoo SJ, Lee EB. 1986b. Pharmacological studies on Prunellae Herba and Thesii Herba ( II ). On central nervous and diuretic actions. Kor J Pharmacogn 17: 242-247.
37 Au TK, Lam TL, Ng TB, Fong WP, Wan DC. 2001. A comparison of HIV-1 integrase inhibition by aqueous and methanol extracts of Chinese medicinal herbs. Life Sci 68: 1687-1694.   DOI
38 Fang X, Yu MM, Yuen WH, Zee SY, Chanq RC. 2005. Immune modulatory effects of Prunella vulgaris L. on monocytes/macrophages. Int J Mol Med 16: 1109-1116.
39 Jung YB, Roh KJ, Jung JA, Jung K, Yoo H, Cho YB, Kwak WJ, Kim DK, Kim KH, Han CK. 2001. Effect of SKI 306X, a new herbal anti-arthritic agent, in patients with osteoarthritis of the knee: a double-blind placebo controlled study. Am J Chin Med 29: 485-491.   DOI
40 Lamaison JL, Petitjean-Freytet C, Carnat A. 1991. Medicinal Lamiaceae with antioxidant properties, a potential source of rosmarinic acid. Pharm Acta Helv 66: 185-188.
41 Lee H, Lin JY. 1988. Antimutagenic activity of extracts from anticancer drugs in Chinese medicine. Mutat Res 204: 229-234.   DOI
42 Wiseman H. 1996. Dietary influences on membrane function; important in protection against oxidative damage and disease. Nutr Biochem 7: 2-6.   DOI
43 Nanayama M. 1996. Antibacterial substances in food. Jpn J Food Microbial 12: 209-213.   DOI
44 Ames BN, Saul RL. 1987. Oxidative DNA damage, cancer and aging. Oxygen and human disease. Ann Inter Med 107: 536-539.
45 Osborn-Barnes HT, Akoh CC. 2003. Effect of ${\alpha}$-tocopherol, ${\beta}$-carotene, and isoflavones on lipid oxidation of structured lipid-based emulsions. J Agric Food Chem 51: 6856-6860.   DOI
46 Berg RD. 1998. Probiotics, prebiotics or conbiotics. Trend Microbial 6: 9-92.   DOI
47 Sandra J. 1963a. Phytochemical studies of Prunella vulgaris and Prunella grandiflora- I. Saponin and triterpene compounds. Dissertations Pharm 15: 333-341.