Browse > Article
http://dx.doi.org/10.3746/jkfn.2009.38.5.653

Isolation and Purification of Decursin and Decursinol Angelate in Angelica gigas Nakai  

Kim, Kang-Min (Dept. of Smart Foods and Drugs, Inje University)
Jung, Jae-Yeon (Dept. of Smart Foods and Drugs, Inje University)
Hwang, Sung-Woo (Dept. of Smart Foods and Drugs, Inje University)
Kim, Myo-Jeong (Dept. of Smart Foods and Drugs, Inje University)
Kang, Jae-Seon (Dept. of Pharmacy, Kyungsung University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.38, no.5, 2009 , pp. 653-656 More about this Journal
Abstract
This paper is intended as an investigation of the method of extraction and the analysis by high-performance liquid chromatography mass spectroscopy of decursin and decursinol angelate in the dried root of Angelica gigas Nakai. The extracted decursin and decursinol angelate were the purity of >95% using 60% ethanol at $-20^{\circ}C$ for 12 hours by HPLC analysis. Decursin and decursinol angelate were efficiently isolated using recycling HPLC. The purity of isolated decursin and decursinol angelate was identified as 99.97 and 99.40% by HPLC analysis, respectively. The molecular weights of Decursin and decursinol angelate were also identified as m/z=329 ($[M+H]^+$) and m/z=351 ($[M+Na]^+$) by mass spectroscopy.
Keywords
decursin; decursinol angelate; Angelica gigas Nakai; recycling HPLC;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By SCOPUS : 3
연도 인용수 순위
1 Ahn MJ, Lee MK, Kim YC, Sung SH. 2008. The simultaneous determination of coumarins in Angelica gigas root by high performance liquid chromatography-diode array detector coupled with electrospray ionization/mass spectrometry. J Pharmaceut Biomed 46: 258-266   DOI   ScienceOn
2 Yook CS. 1990. Coloured medicinal plants of Korea. Academy Book Co., Seoul. p 390
3 Kang SY, Lee KY, Sung SH, Park MJ, Kim YC. 2001. Coumarins isolated from Angelica gigas inhibit acetylcholinesterase structure-activity relationships. J Nat Prod 64: 683-685   DOI   ScienceOn
4 Lee JH, Choi YS, Kim JH, Jeong HG, Kim DH, Yun MY, Kim JS, Lee SH, Cho SH, Shen GN, Kim EG, Jin WY, Song GY. 2006. A mass preparation method of (+)-decursinol from the roots of Angelica gigas. Yakhakhoeji 50: 172-176
5 Bae EA, Han MJ, Kim NJ, Kim DH. 1998. Anti-Helicobacter pylori activity of herbal medicines. Biol Pharm Bull 21: 990-992   DOI   ScienceOn
6 Lee SH, Shin DS, Kim JS, Oh KB, Kang SS. 2003. Antibacterial courmins from Angelica gigas roots. Arch Pharm Res 26: 449-452   DOI   ScienceOn
7 Yim DS, Singh RP, Agarwal C, Lee SY, Chi HJ. 2005. A novel anticancer agent, decursin, induces G1 arrest and apoptosis in human prostate carcinoma cells. Cancer Res 65: 1035-1044
8 Ahn KS, Sim WS, Kim IH. 1996. Decursin: a cytotoxic agent and protein kinase C activator from the root of Angelica gigas. Planta Med 62: 7-9   DOI   ScienceOn
9 Ryu KS, Yook CS. 1967. Studies on the coumarins of the root of Angelica gigas Nakai. Yakhakhoeji 11: 22-26
10 Kim HH, Ahn KS, Han H, Choung SY, Choi SY, Kim IH. 2005. Decursin and PDBu: two PKC activators distinctively acting in the megakaryocytic differentiation of K562 human erythroleukemia cells. Leuk Res 29: 1407-1413   DOI   ScienceOn
11 Yim D, Singh RP, Agarwal C, Lee S, Chi H, Agarwal R. 2005. A novel anticancer agent, decursin, induces G1 arrest and apoptosis in human prostate carcinoma cells. Cancer Res 65: 1035-1044
12 Park KW, Choi SR, Shon MY, Jeong IY, Kang KS, Lee ST, Shim KH, Seo KI. 2007. Cytotoxic effects of decursin from Angelica gigas Nakai in human cancer cells. J Korean Soc Food Sci Nutr 36: 1385-1390   과학기술학회마을   DOI   ScienceOn
13 Lim JD, Kim IH, Kim HH, Ahn KS, Han HG. 2001. Enantioselective synthesis of decursinol angelate and decursin. Tetrahedron Lett 42: 4001-4003   DOI   ScienceOn
14 Lee SH, Lee YS, Jung SH, Shin KH, Kim BK, Kang SS. 2003. Anti-tumor activities of decursinol angelate and decursin from Angelica gigas. Arch Pharm Res 26: 727-730   DOI   ScienceOn