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http://dx.doi.org/10.3746/jkfn.2009.38.3.297

Antioxidative Effects and Anti-proliferative Effects of MeOH, BuOH and Ethyl Acetate Fractionated from Stephania delavayi Diels  

Li, Yong-Chun (Korea Institute of Oriental Medicine)
Kim, Kyoung-Hee (Dept. of Food and Nutrition, Chungnam National University)
Xu, Hong-De (Korea Institute of Oriental Medicine)
Park, Dae-Hun (Korea Institute of Oriental Medicine)
Choi, Yeon-Shik (Korea Bio Polytechnic)
Hwang, Hye-Rim (Dept. of Food and Nutrition, Chungnam National University)
Lee, Min-Jae (School of Veterinary Medicine, Kangwon National University)
Choi, Jong-Jin (Crops Research Division, Chungcheongnam-do Agricultural Research and Extension Services)
Kwon, Myung-Sang (School of Veterinary Medicine, Kangwon National University)
Yook, Hong-Sun (Dept. of Food and Nutrition, Chungnam National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.38, no.3, 2009 , pp. 297-301 More about this Journal
Abstract
Stephania delavayi Diels. (S. delavayi Diels.) has been used as a drug for pain-relieving and acute gastroenteritis treatment in China. Because the major therapeutic mechanism of anti-inflammatory drug is to inhibit the cyclooxygenase (COX)-2 and because COX-2 proteins inhibit apoptosis, COX-2 inhibitor has been thought as the anticancer drug candidate. For this reason, we examined S. delavayi Diels. as an anticancer drug. S. delavayi Diels. was fractionated with methanol and then partitioned with ethyl acetate, n-butanol and water. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and reducing power. DPPH radical scavenging activities of the crude fractions at the concentration of $1,000{\mu}g/mL$ were 75.23% (n-butanol), 68.11% (methanol), 63.58% (ethyl acetate), and 50.13% (water). The reducing power increased according to the concentration in dose-dependent manner. Also, when the antiproliferation effects of each fraction against human breast cancer cell-lines MDA-MB-231 and MCF-7 were examined, methanol extract, n-butanol fraction and ethyl acetate fraction exhibited cell proliferative inhibition effects in both cell-lines whereas water fraction did not. Among the crude fractions, the n-butanol fraction exhibited the most potent anti-proliferation effect. In conclusion, fractions from S. delavayi Diels. are promising anticancer drug candidates.
Keywords
Stephania delavayi Diels; fraction; antioxidant; anti-proliferation against cancer cell-line;
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1 Meng LH, Zhang H, Hayward L, Takemura H, Shao RG, Pommier Y. 2004. Tetrandrine Induces Early $G_1$ Arrest in Human Colon Carcinoma Cells by Down-Regulating the Activity and Inducing the Degradation of $G_1S_-$Specific Cyclin-Dependent Kinases and by Inducing p53 and $p21^{Cip1}$. Cancer Res 64: 9086-9092   DOI   ScienceOn
2 Klaunig JE, Kamendulis LM. 2004. The role of oxidative stress in carcinogenesis. Annu Rev Pharmacol Toxicol 44: 239-267   DOI   ScienceOn
3 Takamatsu S, Hodges TW, Rajbhandari I, Gerwick WH, Hamann MT, Nagle DG. 2003. Marine natural products as novel antioxidant prototypes. J Nat Prod 66: 605-608   DOI   ScienceOn
4 Oyaizu M. 1986. Studies on product of browning reaction prepared from glucose amine. Jap J Nutr 44: 307-315   DOI
5 Hayase F, Hirashima S, Okamoto G, Kato H. 1989. Scavenging of active oxygens by melanoidins. Agric Biol Chem 53: 3383-3385   DOI
6 Editorial Board. 1999. The Dictionary of Traditional Chinese Medicine. Scientific Technological Press, Beijing
7 Halicka D, Ita M, Tanaka T, Kurose A, Darzynkiewicz Z. 2008. Biscoclaurine alkaloid cepharanthine protects DNA in TK6 lymphoblastoid cells from constitutive oxidative damage. Pharmacol Rep 60: 93-100
8 Kikukawa Y, Okuno Y, Tatetsu H, Nakamura M, Harada N, Ueno S, Kamizaki Y, Mitsuya H, Hata H. 2008. Induction of cell cycle arrest and apoptosis in myeloma cells by cepharanthine, a biscoclaurine alkaloid. Int J Oncol 33: 807-814
9 Yoo SJ, Kim SH, Jun MS, Oh HT, Choi HJ, Ham SS. 2007. Antioxidative, antimutagenic and cytotoxic effects of Prunus armeniaca extracts. Korean J Food Preserv 14: 220-225   과학기술학회마을
10 Aoshima H, Tsunoue H, Koda H, Kiso Y. 2004. Aging of whisky increases 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. J Agric Food Chem 52: 5240-5244   DOI   ScienceOn
11 Song HS, Park YH, Jung SH, Kim DP, Jung YH, Lee MK, Moon KY. 2006. Antioxidant activity of extracts from Smilax china root. J Korean Soc Food Sci Nutr 35: 1133-1138   과학기술학회마을   DOI   ScienceOn
12 Lee JC, Lim KT, Jang YS. 2002. Identification of Rhus verniciflua Stokes compounds that exhibit free radical scavenging and anti-apoptotic properties. Biochem Biophys Acta 1570: 181-191   DOI   ScienceOn
13 Lim KT, Lee JC. 1999. Bioactive utility of the extracts from Rhus verniciflua Stokes (RVS): Biological function of the extracts from RVS. Korean J Food Sci Technol 31: 238-245   과학기술학회마을
14 Kim OK. 2005. Antidiabetic and antioxidative effects of Corni fructus in streptozotocin-induced diabetic rats. J Korean Oil Chemists Soc 22: 157-167   과학기술학회마을
15 Choi DY, Do JH, Lee KS, Yang CB. 1994. Changes in hydrogen donating activities of the extract from Hololeion maximowiczii root by drying methods. Korean J Food Sci Technol 26: 147-151   과학기술학회마을
16 Jeong CH, Shim KH. 2006. Chemical composition and antioxidative activities of Platycodon grandiflorum leaves and stems. J Korean Soc Food Sci Nutr 35: 511-515   과학기술학회마을   DOI   ScienceOn
17 DiPaola RS, Zhang H, Lambert GH, Meeker R, Licitra E, Rafi MM, Zhu BT, Spaulding H, Goodin S, Toledano MB, Hait WN, Gallo MA. 1998. Clinical and biologica activity of an estrogenic herbal combination (PC-SPES) in prostate cancer. N Egnl J Med 339: 785-791   DOI   ScienceOn