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http://dx.doi.org/10.3746/jkfn.2009.38.11.1535

Immune Stimulation and Anti-Metastasis of Crude Polysaccharide from Submerged Culture of Hericium erinaceum in the Medium Supplemented with Korean Ginseng Extracts  

Kim, Hoon (Division of Food and Biotechnology, Chungju National University)
Park, Chang-Kyu (Division of Food and Biotechnology, Chungju National University)
Jeong, Jae-Hyun (Division of Food and Biotechnology, Chungju National University)
Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
Lee, Hyeon-Yong (Dept. of Biomaterials Engineering, Kangwon National University)
Yu, Kwang-Won (Division of Food and Biotechnology, Chungju National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.38, no.11, 2009 , pp. 1535-1542 More about this Journal
Abstract
To find the new use of Korean ginseng and mushroom, crude polysaccharides were prepared from submerged cultures of Hericium erinaceum in the medium supplemented with Korean ginseng extracts. When we fractionated crude polysaccharides (HE-GE-CP-1, 3, and 5) from hot-water extracts of submerged cultures of H. erinaceum with ginseng extracts (1%, 3%, and 5% addition of total medium), the yields of HE-GE-CP-1, 3, and 5 were identified at 5.7, 5.1, and 4.8%, respectively. Among crude polysaccharide fractions, HE-GE-CP-5 was significantly higher (1.89-fold of the saline control) than those of HE-GE-CP-1 (1.64-fold) or HE-GE-CP-3 (1.76-fold) on mitogenic activity of splenocytes. HE-GE-CP-5 also had the more potent bone marrow cell proliferation (1.83-fold) rather than HE-CP or HE-GE-CP-1 or HE-GE-CP-3 (1.59- or 1.44- or 1.69-fold, respectively), and anti-metastatic activity as anti-cancer effect showed the highest prophylactic value (72.4% inhibition of tumor control) in 5% supplementation of ginseng extract. However, the lysosomal phosphatase of macrophage was significantly stimulated after HE-GE-CP-3 treatment (2.03-fold). In addition, the immunostimulating and anti-metastatic crude polysaccharide, HE-GE-CP-5, contained mainly neutral sugars (63.2%) with considerable amounts of uronic acid (19.3%) and a small amount of proteins (8.8%). HE-GE-CP-5 can stimulate immune system to inhibit tumor metastasis, and its anti-tumor metastasis may be associated with macrophages, splenocytes and Peyer's patch cells activation.
Keywords
Hericium erinaceum; Korean ginseng extract; submerged culture; crude polysaccharide; immunostimulation; anti-metastasis;
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