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http://dx.doi.org/10.3746/jkfn.2009.38.11.1492

Effect of Ascorbic Acid, Silicon, Fe, Proline and Lysine on Proliferation and Collagen Synthesis in the Human Dermal Fibroblast Cell (HS27)  

Kim, Sun-Ah (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Lee, Jin-Ah (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Kim, Jung-Min (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Kim, Hyun-Ae (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Kim, Young-Ae (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Yun, Hye-Jeong (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Cho, Yun-Hi (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.38, no.11, 2009 , pp. 1492-1498 More about this Journal
Abstract
In the dermis, fibroblast plays an important role in the turnover of the dermal extracellular matrix. Collagen I and III, which are the most important dermal proteins of the extracellular matrix, function as a stabilizing scaffold of dermal connective tissues, as well as a regulator of differentiation and migration of dermal cells. In this study, we investigated the effect of various nutrients, such as ascorbic acid, silicon, Fe, lysine and proline which function as cofactors or building blocks on collagen synthesis. When the physiological concentrations of ascorbic acid (0-100 ${\mu}M$), silicon (0-50 ${\mu}M$), Fe (0-50 ${\mu}M$), lysine (0-150 ${\mu}M$) and proline (0-300 ${\mu}M$) were treated at HS27 for either 3 or 5 days, 5 day treatment of ascorbic acid at the low concentration (5-10 ${\mu}M$) increased the expression of collagen I and III protein by 115-1300% without increasing cell proliferation. 3 or 5 days treatment of Fe increased the expression of collagen I and III proteins up to 323% in parallel with cell proliferation by 164%. However, cell proliferation and expression of collagen I and III protein in silicon treated HS27 did not differ. Proline and lysine only increased cell proliferation up to 247.9%. Taken together, we demonstrate that the physiological concentrations of ascorbic acid and Fe enhance the expression of collagen I and III protein for treatment of 3 or 5 days.
Keywords
human dermal fibroblast; collagen; ascorbic acid; silicon; Fe;
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1 Ruiz I.G, de la Torre P, Diaz T, Esteban E, Morillas J.D, Munoz-Yague T, Solis-Herruzo J.A. 2000. Sp family of transcription factors is involved in iron-induced collagen alpha1 (I) gene expression. DNA Cell Biol 19: 167-178   DOI   ScienceOn
2 Gardi C, Arezzini B, Fortino V, Comporti M. 2002. Effect of free iron on collagen synthesis, cell proliferation and MMP-2 expression in rat hepatic stellate cells. Biochem Pharmacol 64: 1139-1145   DOI   ScienceOn
3 Bauer E.A, Uitto J. 1982. Skin, collagen in health and disease. JB Weiss, MlV Jayson, eds. Churchill Livingston, Edinburgh, UK. p 474-487
4 Tinker D, Rucker R.B. 1985. Accumulation and chemical modification of connective tissue proteins. Physiol Rev 65: 607-657   DOI
5 Berg R.A, Kerr J.S. 1992. Nutritional aspects of collagen metabolism. Annu Rev Nutr 12: 369-390   DOI   ScienceOn
6 Passoja K, Rautavuoma K, Ala-Kokko L, Kosonen T, Kivirikko K.I. 1998. Cloning and characterization of a third human lysyl hydroxylase isoform. Proc Natl Acad Sci USA 95: 10482-10486   DOI   ScienceOn
7 Seaborn CD, Nielsen FH. 2002. Silicon deprivation decreases collagen formation in wounds and bone, and ornithine transaminase enzyme activity in liver. Biol Trace Elem Res 89: 251-261   DOI   ScienceOn
8 Ishikawa T, Ishikawa O, Miyachi Y. 1995. Measurement of skin elastic properties with a new suction device (Ⅰ): Relationship to age, sex and the degree of obesity in normal individuals. J Dermatol 22: 713-717   DOI
9 Pinnel S.R, Murad S, Darr D. 1987. Induction of collagen synthesis by ascorbic acid. A possible mechanism. Arch Dermatol 123: 1684-1686   DOI   ScienceOn
10 Etienne-Manneville S, Hall A. 2001. Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCzeta. Cell 106: 489-498   DOI   ScienceOn
11 Che P, Xu J, Shi H, Ma Y. 1995. Quantitative determination of serum iron in human blood by high performance capillary electrophoresis. J Chromato B 669: 45-51   DOI   ScienceOn
12 Jemec G.B, Jemec B, Jemec B.I, Serup J. 1990. The effect of superficial hydration on the mechanical properties of human skin in vivo: implications for plastic surgery. Plast Reconstr Surg 85: 100-103   DOI
13 Kang S.A, Jang K.H, Cho Y.H, Hong K.H, Kong S.H, Choue R.W. 2001. High performance liquid chromatographic analysis of isoflavones in soybean and blackbean. J Arache 8: 44-48
14 Bernard M.P, Myers J.C, Chu M.L, Ramirez F, Eikenberry E.F, Prockop D.J. 1983. Structure of a cDNA for the pro alpha 2 chain of human type Ⅰ procollagen. Comparison with chick cDNA for pro alpha 2 (I) identifies structurally conserved features of the protein and the gene. Biochemistry 22: 1139-1145   DOI   ScienceOn
15 Elsner P, Wilhelm D, Maibach H.I. 1990. Mechanical properties of human forearm and vulvar skin. Br J Dermatol 122: 607-614   DOI   ScienceOn
16 Groff J.L, Gropper S.S. 1999. Advanced nutrition and human metabolism. 3rd ed. Peter Marshall Publisher, Wadsworth/ Thomson Learing, Belmont, USA. p 251
17 Shibayama H, Hisama M, Matsuda S, Ohtsuki M, Iwaki M. 2008. Effect of a novel ascorbic derivative, disodium isostearyl 2-O-L-ascorbyl phosphate on human dermal fibroblasts: increased collagen synthesis and inhibition of MMP-1. Biol Pharm Bull 31: 563-568   DOI   ScienceOn
18 Uitto J, Olsen D.R, Fazio M.J. 1989. Extracellular matrix of the skin: 50 years process. J Invest Dermatol 92: 61s-77s   DOI
19 Nimni M.E, Harkness R.D. 1993. Molecular structure and functions of collagen. In Collagen. Nimni ME, ed. CRC Press, Florida. p 1-3
20 Kim N.M, Koo B.S, Lee S.K, Hwang E.I, So S.H, Do J.H. 2007. Effect of Korean red ginseng on collagen biosynthesis and MMP-1 activity in human dermal fibroblast. J Ginseng Res 31: 86-92   과학기술학회마을   DOI   ScienceOn
21 Tajima S, Pinnell S.R. 1996. Ascorbic acid preferentially enhances type Ⅰ and Ⅲ collagen gene transcription in human skin fibroblasts. J Derm Sci 11: 250-253   DOI   ScienceOn
22 Walingo K.M. 2005. Role of vitmin C (ascorbic acid) on human health－A review. African J Food Agric Nutr Develop (AJFAND) 5: 1-13
23 Murad S, Grove D, Lindberg KA, Reynolds G, Sivarajah A, Pinnell S.R. 1981. Regulation of collagen synthesis by ascorbic acid. Proc Natl Acd Sci USA 78: 2879-2882   DOI   ScienceOn
24 Reffitt D.M, Ogston N, Jugdaohsingh R, Cheung H.F, Evans B.A, Thompson R.P, Powell J.J, Hampson G.N. 2003. Orthosilicic acid stimulates collagen type Ⅰ synthesis and osteoblastic differentiation in human osteoblast-like cells in vitro. Bone 32: 127-135   DOI   ScienceOn
25 Carlisle E.M. 1981. Silicon: a requirement in bone formation independent of vitamin D1. Calcif Tissue Int 33: 27-34   DOI   ScienceOn
26 Dionisi Vici C, De Felice L, E.l Hachem M, Bottero S, Rizzo C, Paoloni A, Goffredo B, Sabetta G, Caniglia M. 1998. Intravenous immune globulin in lysinuric protein intolerance. J Inherit Metab Dis 21: 95-102   DOI   ScienceOn
27 Ala-Kokko L, Kontusaari S, Baldwin C.T, Kuivaniemi H, Prockop D.J. 1989. Structure of cDNA clones coding for the entire prepro alpha 1 (Ⅲ) chain of human type Ⅲ procollagen. Differences in protein structure from type Ⅰ procollagen and conservation of codon preferences. Biochem J 260: 509-516
28 Duarte T.L, Cooke M.S, Jones G.D. 2009. Gene expression profiling reveals new protective roles for vitamin C in human skin cells. Free Radic Biol Med 46: 78-87   DOI   ScienceOn