Browse > Article
http://dx.doi.org/10.3746/jkfn.2009.38.10.1347

Modulation of Ethanol-Induced P450 Enzyme Activities and Antioxidants in Mice by Hordeum vulgare Extract  

Lee, Yoo-Hyun (Dept. of Food Science and Nutrition, University of Suwon)
Lee, Jeong-Min (Dept of Medical Nutrition, Kyunghee University)
Im, Eun-Jeong (Dept of Food Service, Hanyang Women's College)
Jun, Woo-Jin (Dept. of Food and Nutrition, Chonnam National University)
Cho, Hong-Yon (Dept of Food and Biotechnology, Korea University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.38, no.10, 2009 , pp. 1347-1352 More about this Journal
Abstract
The effects of methanol extract of barely (Hordeum vulgare) on alcohol-induced damages of liver were investigated in male ICR mice. Mice were divided into three groups, control, ethanol, and ethanol plus 0.15% of barley extract. After four weeks of ethanol feeding, ethanol group significantly increased the P450 content, CYP2E1 and CYP1A2 enzyme activities, whereas ethanol plus barely group markedly decreased to levels similar to control group. Catalase activity in ethanol group was significantly lower than that in control group; however, ethanol plus barely group stimulated catalase activity as well as SOD activity significantly. These results indicated that barely extract modulated P450 enzymes for ethanol-induced liver damage and might be useful in developing functional food for alcoholic liver damage.
Keywords
alcohol; cytochrome P450; Hordeum vulgare; hepatoprotective effect; oxidative damage;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By SCOPUS : 3
연도 인용수 순위
1 Kessova IG, Leo MA, Lieber CS. 2001. Effect of beta-carotene on hepatic cytochrome P-450 in ethanol-fed rats. Alcohol Clin Exp Res 25: 1368-1372.   DOI
2 심창구. 1999. 생물약제학. 서울대학교 출판부, 서울. p 296-299.
3 Lu Y, Cederbaum AI. 2008. CYP2E1 and oxidative liver injury by alcohol. Free Radic Biol Med 44: 723-738.   DOI   ScienceOn
4 Rizzo N, Hispard E, Dolbeault S, Dally S, Leverge R, Girre C. 1997. Impact of long-term ethanol consumption on CYP1A2 activity. Clin Pharmacol Ther 62: 505-509.   DOI
5 Schoedel KA, Tyndale RF. 2003. Induction of nicotine-metabolizing CYP2B1 by ethanol and ethanol-metabolizing CYP2E1 by nicotine: summary and implications. Biochim Biophys Acta 1619: 283-290.   DOI   ScienceOn
6 Mari M, Cederbaum AI. 2001. Induction of catalase, alpha, and microsomal glutathione S-transferase in CYP2E1 overexpressing HepG2 cells and protection against short-term oxidative stress. Hepatology 33: 652-661.   DOI   ScienceOn
7 Zdunczyk Z, Flis M, Zielinski H, Wroblewska M, Antoszkiewicz Z, Juskiewicz J. 2006. In vitro antioxidant activities of barley, husked oat, naked oat, triticale, and buckwheat wastes and their influence on the growth and biomarkers of antioxidant status in rats. J Agric Food Chem 54: 4168-4175.   DOI   ScienceOn
8 Omura T, Sato R. 1964. The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J Biol Chem 239: 2370-2378.
9 Hong H, Jai Maeng W. 2004. Effects of malted barley extract and banaba extract on blood glucose levels in genetically diabetic mice. J Med Food 7: 487-490.   DOI   ScienceOn
10 Kamimura A, Takahashi T. 2002. Procyanidin B-3, isolated from barley and identified as a hair-growth stimulant, has the potential to counteract inhibitory regulation by TGFbeta1. Exp Dermatol 11: 532-541.   DOI   ScienceOn
11 Hidestrand M, Shankar K, Ronis MJ, Badger TM. 2005. Effects of light and dark beer on hepatic cytochrome P-450 expression in male rats receiving alcoholic beverages as part of total enteral nutrition. Alcohol Clin Exp Res 29: 888-895.   DOI   ScienceOn
12 Aebi H. 1984. Catalase in vitro. Methods Enzymol 105: 121-126.   DOI
13 You Y, Jung KY, Lee YH, Jun W, Lee BY. 2009. Hepatoprotective effects of Hovenia dulcis fruit on ethanol-induced liver damage in vitro and in vivo. J Korean Soc Food Sci Nutr 38: 154-159.   과학기술학회마을   DOI   ScienceOn
14 Caro AA, Cederbaum AI. 2004. Oxidative stress, toxicology, and pharmacology of CYP2E1. Annu Rev Pharmcol Toxicol 44: 27-42.   DOI   ScienceOn
15 Choi HB. 2009. Alcoholic liver disease. Korean J Gastroenterol 53: 275-282.   DOI   ScienceOn
16 Jaeschke HG, Gores J, Cederbaum AI, Hinson JA, Pessayre S, Lemasters JJ. 2002. Mechanism of hepatotoxicity. Toxicol Sci 65: 166-176.   DOI   ScienceOn
17 Gaudineau C, Beckerman R, Welbourn S, Auclair K. 2004. Inhibition of human P450 enzymes by multiple constituents of the Ginkgo biloba extract. Biochem Biophys Res Commun 318: 1072-1078.   DOI   ScienceOn
18 Schoedel KA, Tyndale RF. 2003. Induction of nicotine-metabolizing CYP2B1 by ethanol and ethanol-metabolizing CYP2E1 by nicotine: summary and implications. Biochim Biophys Acta 1619: 283-290.   DOI   ScienceOn
19 Seog HM, Seo MS, Kim SR, Park YK, Lee YT. 2002. Characteristics of barely polyphenol extract (BPE) separated from pearling by-products. Korean J Food Sci Technol 5: 775-779.
20 Gillam EM, Baba T, Kim BR, Ohmori S, Guengerich FP. 1993. Expression of modified human cytochrome P450 3A4 in Escherichia coli and purification and reconstitution of the enzyme. Arch Biochem Biophys 305: 123-131.   DOI   ScienceOn
21 Rizzo N, Hispard E, Dolbeault S, Dally S, Leverge R, Girre C. 1997. Impact of long-term ethanol consumption on CYP1A2 activity. Clin Pharmacol Ther 62: 505-509.   DOI
22 McLean L, Soto U, Agama K, Francis J, Jimenez R, Pommier Y, Sowers L, Brantley E. 2008. Aminoflavone induces oxidative DNA damage and reactive oxidative species- mediated apoptosis in breast cancer cells. Int J Cancer 122: 1665-1674.   DOI   ScienceOn
23 McCord JM, Fridovich I. 1969. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 244: 6049-6055.
24 Kim SN, Kim MS, Park HS. 2007. Effects of dietary conjugated linoleic acid (CLA) on antioxidant system in the liver of chronically ethanol-treated rats. Korean J Nutrition 40: 105-110.   과학기술학회마을
25 Shim SI, Chung KW, Lee JM, Hwang KT, Sone J, Hong BS, Cho HY, Jun W. 2006. Hepatoprotective effects of black rice on superoxide anion radicals in HepG2 cell. Food Sci Biotechnol 15: 993-996.
26 Zuber R, Modriansky M, Dvorak Z, Rohovsky P, Ulrichova J, Simanek V, Anzenbacher P. 2002. Effect of silybin and its congeners on human liver microsomal cytochrome P450 activities. Phytother Res 6: 632-638.   DOI   ScienceOn
27 Wolf KK, Wood SG, Allard JL, Hunt JA, Gorman N, Walton-Strong BW, Szakacs JG, Duan SX, Hao Q, Court MH, von Moltke LL, Greenblatt DJ, Kostrubsky V, Jeffery EH, Wrighton SA, Gonzalez FJ, Sinclair PR, Sinclair JF. 2007. Role of CYP3A and CYP2E1 in alcohol-mediated increases in acetaminophen hepatotoxicity: comparison of wild-type and Cyp2e1(-/-) mice. Drug Metab Dispos 35: 1223-1231.   DOI   ScienceOn
28 Wilson TA, Nicolosi RJ, Delaney B, Chadwell K, Moolchandani V, Kotyla T, Ponduru S, Zheng GH, Hess R, Knutson N, Curry L, Kolberg L, Goulson M, Ostergren K. 2004. Reduced and high molecular weight barley beta- glucans decrease plasma total and non-HDL-cholesterol in hypercholesterolemic Syrian golden hamsters. J Nutr 134: 2617-2622.   DOI
29 Caro AA, Cederbaum AI. 2004. Oxidative stress, toxicology, and pharmacology of CYP2E1. Annu Rev Pharmcol Toxicol 44: 27-42.   DOI   ScienceOn
30 Lieber CS. 2004. CYP2E1: ASH to NASH. Hepatol Res 28: 1-11.   DOI   ScienceOn