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http://dx.doi.org/10.3746/jkfn.2009.38.10.1317

Induction of Apoptosis by Citrus grandis Osbeck Peel (CGP) Extract in HL60 Cells  

Hyon, Jae-Seok (Dept. of Food and Nutrition, Jeju College of Technology)
Kang, Sung-Myung (School of Marine Biomedicinal Sciences, Jeju National University)
Kim, Areum-Daseul (School of Marine Biomedicinal Sciences, Jeju National University)
Oh, Myung-Cheol (Dept. of Tourism Hotel Culinary Art, Jeju College of Technology)
Oh, Chang-Kyung (Dept. of Tourism Hotel Culinary Art, Jeju College of Technology)
Kim, Dong-Woo (Central Research Center, Natural F&P Co., Ltd.)
Jeon, You-Jin (School of Marine Biomedicinal Sciences, Jeju National University)
Kim, Soo-Hyun (Dept. of Food Bioengineering, Jeju National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.38, no.10, 2009 , pp. 1317-1323 More about this Journal
Abstract
In the present study, we investigated the anti-proliferation activity of Citrus grandis Osbeck peel (CGP) in HL60 (human promyelocytic leukemia) cells. It was found that 80% ethanol extract of CGP could inhibit the cell growth in a dose-dependent manner ($250{\sim}1,000{\mu}g/mL$), which was associated with morphological changes and apoptotic cell death such as depolarized mitochondrial membrane, formation of apoptotic bodies and increased populations of apoptotic sub-G1 phase. The results indicate that CGP extract inhibits the growth of HL60 cancer cells by the induction of apoptosis, which may be mediated by its ability to change the Bcl family proteins and increase the activation of caspase-3 and PARP. Therefore, it is suggested that CGP has the potential to provide a remarkable natural defense against the proliferation of HL60 cells.
Keywords
Citrus grandis Osbeck peel; anti-proliferation; apoptosis; HL60;
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