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http://dx.doi.org/10.3746/jkfn.2008.37.9.1114

Effect of Epigallocatechin Gallate on Apoptosis in MDA-MB-231 Human Breast Cancer Cells  

Hong, Eun-Jung (Dept. of Food Science and Nutrition, Dankook University)
Kim, Woo-Kyung (Dept. of Food Science and Nutrition, Dankook University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.37, no.9, 2008 , pp. 1114-1119 More about this Journal
Abstract
Among the numerous polyphenols isolated from green tea, epigallocatechin gallate (EGCG) is a predominate and is considered to be a major therapeutic agent. To elucidate the mechanical insights of anti-tumor effect, EGCG was applied to human breast cancer MDA-MB-231 cells. We investigated the effect of EGCG on protein and mRNA expression of proteins related to cell apoptosis in MDA-MB-231 human breast cancer cell lines. We also identified caspase-3 activity. We cultured MDA-MB-231 cells in the presence of 0, 5, 10, and $20\;{\mu}M$ of EGCG. Protein and mRNA expression of bcl-2 were decreased dose-dependently in cells treated with EGCG. However, protein and mRNA expression of bax were increased (p<0.05). Caspase-3 activities were increased dose-dependently in cells treated with EGCG. These results suggest that EGCG induces cell apoptosis by increase of caspase activity through decreasing of protein and mRNA expression of bcl-2 and increasing of protein and mRNA expression of bax.
Keywords
epigallocatechin gallate; breast cancer; apoptosis; bcl-2 family; caspase;
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