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http://dx.doi.org/10.3746/jkfn.2008.37.8.992

Inhibitory Effect of Conditioned Medium of Silk Fibroin-Treated Osteoblasts in Osteoclast Differentiation  

Yeo, Joo-Hong (Dept. of Agricultural Biology, National Institute of Agricultural Science and Technology)
Park, Kyung-Ho (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Ju, Won-Chul (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Lee, Jin-Ah (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Lee, Kwang-Gill (Dept. of Agricultural Biology, National Institute of Agricultural Science and Technology)
Woo, Soon-Ok (Dept. of Agricultural Biology, National Institute of Agricultural Science and Technology)
Han, Sang-Mi (Dept. of Agricultural Biology, National Institute of Agricultural Science and Technology)
Kweon, Hae-Yong (Dept. of Agricultural Biology, National Institute of Agricultural Science and Technology)
Kim, Sung-Su (Dept. of Anatomy, Chung-Ang University)
Cho, Yun-Hi (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.37, no.8, 2008 , pp. 992-997 More about this Journal
Abstract
In this study, we investigated the indirect effect of silk-fibroin on osteoclastic differentiation of RAW264.7 cells. The conditioned medium were collected from MC3T3-E1 osbeoblasts treated with $0.001\;mg/mL{\sim}0.1\;mg/mL$ silk fibroin for 6 days, mixed in 1:1 ratio with osteoclast medium, and then added into RAW264.7 cells with receptor activator of nuclear factor kappa B ligand (RANKL), a differentiation inducer for 3 days. Of osteoclastic cytokines in the conditioned medium, the protein expression of osteoprotegerin (OPG) with silk-fibroin was not significantly different. However, the protein expression of interleukin (IL)-$1{\beta}$ was specifically lower in a dose dependent manner. In RAW264.7 cells, the conditioned medium with silk-fibroin inhibited RANKL induced osteoclastic differentiation as total number of multinucleated tartrate-resistant alkaline phosphatase (TRAP)-positive osteoclasts in a dose dependent manner. Taken together, we demonstrated that the conditioned medium of silk-fibroin treated osteoblasts inhibits RANKL induced differentiation of osteoclasts with inhibiting selective expression of IL-$1{\beta}$.
Keywords
silk-fibroin; conditioned medium; osteoprotegerin; interleukin-$1{\beta}$; osteoclastic differentiation;
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