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http://dx.doi.org/10.3746/jkfn.2008.37.8.979

Isolation and Identification of Antioxidant Substances from the Stems of Butterbur (Petasites japonicus)  

Kim, Min-Young (Division of Food Science, Dong-A University)
Yi, Jung-Hyun (Division of Food Science, Dong-A University)
Hwang, Yun-Yi (Division of Food Science, Dong-A University)
Song, Kyung-Sik (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Jun, Mi-Ra (Division of Food Science, Dong-A University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.37, no.8, 2008 , pp. 979-984 More about this Journal
Abstract
The stems of P etasites japonicus were extracted with ethanol and then partitioned with hexane, chloroform, ethyl acetate, n-butanol and water, successively. The antioxidant potency of five crude fractions were determined using (1) 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, (2) thiobarbituric acid reactive substances (TBARS) assay in the linoleic acid model system, and (3) lipoxygenase inhibition assay. Among the crude fractions, the ethyl acetate fraction exhibited the most potent antioxidant effect. By activity-guided fractionation, compound PJ-4 was isolated from the ethyl acetate fraction through the repeated silica gel open column chromatography. The chemical structure of the isolated compound was determined as kaempferol by $^1H-$ and $^{13}C$-NMR analysis and its antioxidative capacity was further investigated. DPPH radical scavenging activity of the compound was 65.76% at the concentration of $100 \;{\mu}g/mL$. The inhibitory activity of the compound against lipid peroxidation and lipoxygenase exhibited 43.47% and 58.60%, respectively at the concentration of $100\;{\mu}g/mL$. The result suggests that the compound may serve as a useful natural antioxidant and furthermore indicates the possibility of developing the stems of Petasites japonicus as a natural antioxidant source.
Keywords
Petasites japonicus; kaempferol; antioxidant; TBARS; lipoxygenase;
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