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http://dx.doi.org/10.3746/jkfn.2008.37.7.853

The Micronucleus Test of the Diglyceride Preparation with Conjugated Linoleic Acid by Using Mice  

Hong, Soon-Gi (KT&G Central Research Institute)
Chung, Shin-Gyo (College of Agriculture & Life Sciences, Kyungpook National University)
Hyun, Sun-Hee (KT&G Central Research Institute)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.37, no.7, 2008 , pp. 853-857 More about this Journal
Abstract
To assess the clastogenic effects of the diglyceride preparation containing conjugated linoleic acid (DG+CLA) in vivo micronucleus test was performed using ICR mice. Each of the groups consisted of three doses of DG+CLA (500, 1,000 and 2,000 mg/kg, p.o.), Mitomycin C (positive control, 2 mg/kg, i.p.) and negative control (olive oil, 10 mL/kg, p.o.). A slide preparation was made at 24 hours after 1st treatment with DG+CLA. As a result of counting the icronucleated polychromatic erythrocyte (MNPCE) of 2,000 polychromatic erythrocyte (PCE), the number of aberrant cells was not increased in any of the three doses of DG+CLA orally administered. There was no clinical sign connected with administration of DG+CLA. These results indicate that DG+CLA is not capable of inducing micronuclei in vivo mice cells and thus has no genotoxicity in micronucleus.
Keywords
diglyceride preparation containing conjugated linoleic acid; micronucleus; micronucleated polychromatic erythrocyte; polychromatic erythrocyte;
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1 Lee J, Joo ST, Choi BD, Ha YL, Ha JK, Park GB. 1999. The effect of conjugated linoleic acid (CLA) feeding period on CLA content and fatty acid composition of chicken. Korean J Anim Sci 41: 375-386
2 Joseph AS, Miller GD. 2000. Potential health benefits of conjugated linoleic acid. J Nutr 19: 470S-471S
3 Yurawecz MP, Hood JK, Pariza MW. 1995. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108: 19-25   DOI
4 Alder ID. 1984. Cytogenetic tests in mammals. In Mutagenecity testing a practical approach. Venitt S, Parry JM, eds. IRL Press, Oxford. p 275-306
5 Ha YL, Grimm NK, Pariza MW. 1987. Anticarcinogens from fried ground beef: Heataltered derivatives of linoleic acid. Carcinogenesis 8: 1881-1887   DOI   ScienceOn
6 Lin TY. 1999. Conjugated linoleic acid concentration as affected by lactic cultures and added linoleic acid. Food Chem 67: 1-3   DOI   ScienceOn
7 Gniwotta C, Morrow JD, Roberts LJ 2nd, Kühn H. 1997. Prostaglandin F2-like compounds, F2-isoprostanes, are present in increased amounts in human atherosclerotic lesions. Arterioscler Thromb Vasc Biol 17: 3236-3241   DOI   ScienceOn
8 Pariza MW, Ha YL, Benjamin H, Sword JT, Gruter A, Chin SF, Storkson J, Faith N, Albright K. 1991. Formation and action of anticarcinogenic fatty acids. Adv Exp Med Biol 289: 269-272   DOI
9 Macdonald HB. 2000. Conjugated linoleic acid and disease prevention: A review of current knowledge. J Nutr 19: 111S-118S   DOI   ScienceOn
10 Sugano M, Tsujita A, Yamasaki M, Yamada K, Ikeda I, Kritchevsky D. 1997. Lymphatic recovery, tissue distribution and metabolic effects of conjugated linoleic acid in rats. J Nutr Biochem 8: 38-43   DOI   ScienceOn
11 Kastenbaum MA, Bowman KO. 1970. Tables for determining the statistical significance of mutation frequencies. Mutat Res 9: 527-549   DOI   ScienceOn