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http://dx.doi.org/10.3746/jkfn.2008.37.5.555

Mechanism of Growth Inhibition by BCH in HEp2 Human Head and Neck Squamous Cell Carcinoma  

Choi, Bong-Kyu (Dept. of Pharmacology, Wonkwang University School of Medicine)
Jung, Kyu-Yong (Dept. of Pharmacology, Wonkwang University School of Medicine)
Cho, Seon-Ho (Dept. of Oral Physiology and The Second Stage of BK21, Chosun University College of Dentistry)
Kim, Chun-Sung (Dept. of Oral Physiology and The Second Stage of BK21, Chosun University College of Dentistry)
Kim, Do-Kyung (Dept. of Oral Physiology and The Second Stage of BK21, Chosun University College of Dentistry)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.37, no.5, 2008 , pp. 555-560 More about this Journal
Abstract
Amino acid transporters are essential for the growth and proliferation in all living cells. Among the amino acid transporters, the system L amino acid transporters are the major nutrient transport system responsible for the $Na^+$-independent transport of neutral amino acids including several essential amino acids. The L-type amino acid transporter 1 (LAT1), an isoform of system L amino acid transporter, is highly expressed in cancer cells to support their continuous growth and proliferation. 2-Aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH) is a model compound for the study of amino acid transporter as a system L selective inhibitor. We have examined the effect and mechanism of BCH on cell growth suppression in HEp2 human head and neck squamous cell carcinoma. The BCH inhibited the L-leucine transport in a concentration-dependent manner with a $IC_{50}$ value of $51.2{\pm}3.8{\mu}M$ in HEp2 cells. The growth of HEp2 cells was inhibited by BCH in the timeand concentration-dependent manners. The formation of DNA ladder was not observed with BCH treatment in the cells. Furthermore, the proteolytic processing of caspase-3 and caspase-7 in the cells were not detected by BCH treatment. These results suggest that the BCH inhibits the growth of HEp2 human head and neck squamous cell carcinoma through the intracellular depletion of neutral amino acids for cell growth without apoptotic processing.
Keywords
amino acid transporter; cancer cells; BCH; growth inhibition; anti-cancer therapy;
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