Browse > Article
http://dx.doi.org/10.3746/jkfn.2008.37.4.459

Effects of Milk with Boiled-Dried Large Anchovy, Calcium-Fortifying Materials and Fortified-Calcium Milk on Calcium Absorption Rate and Bone Metabolism in Rats  

Jo, Jin-Ho (Korea Food Research Institute)
Kim, Byung-Gi (Korea Food Research Institute)
Han, Chan-Kyu (Korea Food Research Institute)
Jung, Eun-Bong (Korea Food Research Institute)
Cho, Seung-Mock (Korea Food Research Institute)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.37, no.4, 2008 , pp. 459-464 More about this Journal
Abstract
This study was performed to investigate the effect of calcium-rich large anchovy on calcium metabolism in rats for 5 weeks. Experimental animals were randomly assigned to 5 treatments with 14 heads of Spraque Dawley male rats in each group. The experimental diets were as follows; market milk group (M) as control, market milk+calcium-rich large anchovy group (MA), market milk＋calcium carbonate group (MC), market milk+calcium lactate group (ML), and enriched-calcium market milk group (M2), which were formulated with commercially semi-purified rat chow (AIN-diet) to maintain the same level of calcium (1%) in all groups. Femur lengths of M and M2 groups were significantly higher than other groups. Bone mineral density (BMD) and bone mineral content (BMC) and calcium content of femur were the highest in MA group than other groups. In vitro and in vivo calcium absorption rates were high in MA group (7.30% vs 27.50%) compared with those of the other groups. Serum total-cholesterol and HDL-cholesterol levels were significantly different between M group and MA group (p<0.05). Creatinine levels were significantly higher in M, MA and MC groups than in M2 group (p<0.05). Serum calcium, osteocalcin and ALPase activities were higher in calcium-rich large anchovy (MA) group among the treatments, but there was no significant difference. SGOT activity was significantly lower in M2 group than those of M, MA and MC groups (p<0.05). These results may indicate that the calcium-rich large anchovy has enforced the BMD, BMC and calcium absorption rates of in vitro and in vivo compared with the other groups and might be a calcium-enriched food with large anchovy.
Keywords
anchovy; calcium-carbonate; calcium-lactate; calcium absorption rate; bone metabolism; rats;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Folch J, Less M, Stanley GHS. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497-502
2 Aoyama T, Kensuke F, Kiyoharu T, Yukio H, Takashi Y. 2000. Soy protein isolate and its hydrolysate reduce body fat of dietary obese rats and genetically obese mice (Yellow KK). Nutrition 16: 349-354   DOI   ScienceOn
3 L'Heureux-Bouron D, Tome D, Bensaid A, Morens C, Gaudichon C, Fromentin G. 2004. A very high 70%-protein diet does not induce conditioned taste aversion in rats. J Nutr 134: 1512-1515
4 Nagaoka S. 1996. Studies on regulation of cholesterol metabolism induced by dietary food constituents or xenobiotics. J Japan Nutr Soc Food Sci 49: 303-313   DOI
5 Lee HJ, Choi SS, Park MK, An YJ, Seo SY, Kim MC, Hong SH, Hwang TH, Kang DY. 2002. Fenofibrate lowers abdominal and skeletal adiposity and improves insulin sensitivity in OLETF rats. Biochem Biophys Res Commun 296:293-299   DOI   ScienceOn
6 Gil-Campos M, Aguilera CM, Canete R, Gil A. 2006. Ghrelin: a hormone regulating food intake and energy homeostasis. Br J Nutr 96: 201-226   DOI   ScienceOn
7 Bensaid A, Tome D, Gietzen D, Even P, Morens C, Gausseres N, Fromentin G. 2002. Protein is more potent than carbohydrate for reducing appetite in rats. Physiol Behav 75: 577-582   DOI   ScienceOn
8 Lagrange V. 1998. Whey proteins and new fractions as ingredients in functional dairy producs and innovative nutraceuticals. Korean Dairy Techno 16: 106-118
9 Doucet D, Gauthier SF, Foegeding EA. 2001. Rheological characterisation of a gel formed during extensive enzymatic hydrolysis. J Food Sci 66: 711-715   DOI   ScienceOn
10 Calbet JAL, Holst JJ. 2004. Gastric emptying, gastric secretion and enterogastrone response after administration of milk proteins or their peptide hydrolysates in humans. Eur J Nutr 43: 127-139   DOI
11 Lee YS, Park YH, O JH, Kim TJ, Lee HS. 1997. Effect of protein hydrolysates on blood and liver lipids in rats fed fat-enriched diet. Korean J Nutr 30: 614-621
12 Pilvi KT, Riitta K, Minna H, Heikki V, Eero MM. 2007. High-calcium diet with whey protein attenuates bodyweight gain in high-fat-fed C57Bl/6J mice. Br J Nutr 98: 900-907   DOI   ScienceOn
13 O JH, Lee YS. 2002. Hypolipidemic effects of peptide frac tions of casein on serum lipids in rats fed normal or high fat diet. J Korean Soc Food Sci Nutr 31: 263-270   DOI   ScienceOn
14 Claessens M, Saris WHM, van Baak MA. 2008. Glucagon and insulin responses after ingestion of different amounts of intact and hydrolysed proteins. Br J Nutr 2008 Jan 2:1-9 [Epub ahead of print]   DOI   ScienceOn
15 Havel PJ. 2000. Role of adipose tissue in body-weight regulation: mechanisms regulating leptin production and energy balance. Proceedings of the Nutrition Society 59: 359-371
16 Akahoshi A, Koba K, Enmoto R, Nishimura K, Honda Y, Minami M, Yamamoto K, Iwata T, Yamauchi Y, Tsutsumi K, Sugano M. 2005. Combined effects of dietary protein type and fat level on the body fat-reducing activity of conjugated linoleic acid (CLA) in rats. Biosci Ciotechnol Ciochem 69: 2409-2415   DOI   ScienceOn
17 Dimaraki EV, Jaffe CA. 2006. Role of endogenous ghrelin in growth hormone secretion, appetite regulation and metabolism. Rev Endocr Metab Disord 7: 237-249   DOI
18 Belobrajdic DP, Mclntosh GH, Owens JA. 2004. A highwhey- protein diet reduces body weight gain and alters insulin sensitivity relative to red meat in Wistar rats. J Nutr 134: 1454-1458   DOI   ScienceOn
19 Hammond KA, Janes DN. 1998. The effects of increased protein intake on kidney size and function. J Expe Bioll 201: 2081-2090
20 Nagaoka S, Kanamaru Y, Kuzuya Y. 1991. Effect of whey protein and casein on the plasma and liver lipid in rats. Agric Biol Chem 55: 813-818   DOI
21 Nagaoka S, Kanamaru Y, Kojima T, Kuwata T. 1992. Comparative studies on the serum cholesterol lowering action of whey protein and soybean protein in rats. Biol Biotechnol Biochem 56: 1484-1485   DOI
22 Molina OSE, Wagner JR. 2002. Food Res Inter 35:511-518   DOI   ScienceOn
23 Yang HJ, Lee SW. 2003. Emulsifying properties of whey protein hydrolysates. Korean J Food Sci 23: 63-69   과학기술학회마을
24 Reeves PG, Nielsen FH, Fahey Jr GC. 1993. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939-1951
25 Frings CS, Dunn RT. 1970. A colorimetric method for determination of total serum lipids based on the sulfo-phospho- vanillin reaction. Am J Clin Pathol 53: 89-91
26 Ministry of Health and Welfare. 2005. National Health and Nutrition Survey. Seoul
27 Aoyama T, Fukuki K, Toshihiro N, Yukio H, Takashi Y, Kiyoharu T, Michihiro S. 2000. Effect of soy and milk whey protein isolates and their hyrolysates on weight reduction in genetically obese mice. Biosci Biotechnol Biochem 64:2594-2600   DOI   ScienceOn
28 Marshall K. 2004. Therapeutic applications of whey protein. Altern Med Rev 9: 136-156
29 Kimball SR, Jefferson LS. 2001. Regulation of protein synthesis by branched-chain amino acids. Curr Opin Clin Nutr Metab Care 4: 39-43   DOI   ScienceOn
30 Hall WL, Millward DJ, Long SJ, Morgan LM. 2003. Casein and whey exert different effects on plasma amino acid profiles, gastrointestinal hormone secretion and appetite. Br J Nutr 89: 239-248   DOI   ScienceOn
31 Kelley DE, Wing R, Buonocore C, Sturis J, Polonsky D, Fitzsimmons M. 1993. Relative effects of calorie restriction and weight loss in non insulin-dependent diabetes melitus. J Clin Endocrinol Metab 77: 1287-1293   DOI
32 U.S. Department of Health and Human Services. 2006. Health, United States
33 World Health Organization. Fact sheet: obesity and overweight. http://www.who.int/mediacentre/factsheets/fs311/en/ (accessed October, 2005)