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http://dx.doi.org/10.3746/jkfn.2008.37.2.148

Growth Inhibitory and Quinone Reductase Induction Activities of Salicornia herbacea L. Fractions on Human Cancer Cell Lines in vitro  

Jung, Bok-Mi (Dept. of Food Science and Nutrition, Yosu National University)
Park, Jung-Ae (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University)
Bae, Song-Ja (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.37, no.2, 2008 , pp. 148-153 More about this Journal
Abstract
We investigated the growth inhibitory effect of Salicornia herbacea L. (SH) on human cancer cell lines in vitro. SH was extracted with methanol (SHM), followed by further fractionation into four subfractions according to polarity: hexane (SHMH), methanol (SHMM), butanol (SHMB), and aqueous (SHMA) soluble fractions. We determined the growth inhibitory effect of these fractions against human cancer cell lines using MTT assay. Among the four subfractions of SHM, the SHMM showed the strongest cytotoxic effects on cancer cell lines. We also observed quinone reductase (QR)-inducing effect of methanol layer (SHMM) on HepG2 cells and it was determined to be 3.00 at $100\;{\mu}g/mL$ level compared to the control value of 1.0. The SHMM showed the highest induction activity of quinone reductase on HepG2 cells among the partition layers. The present work suggests that SH merits further study to confirm its chemopreventive potential.
Keywords
cancer cell growth inhibition; quinone reductase; Salicornia herbacea L. (SH);
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Times Cited By KSCI : 9  (Citation Analysis)
Times Cited By SCOPUS : 5
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1 Rha ES. 2004. Utilization of halophyte for improving agricultural environment in China. Korean J Plant Res 17: 170-171
2 Min JG, Lee DS, Kim TJ, Park DI. 2002. Chemical composition of Salicornia herbacea L. J Korean Soc Food Sci Nutr 7: 105-107   DOI   ScienceOn
3 Shimizu K. 2000. Effects of salt treatments on the production and chemical composition of salt wort (Salicornia herbacea L.), rhodesgrass and alfalfa. Jpn J Trop Agr 44: 61-67
4 Prochaska HJ, Santamaria AB, Talalay P. 1992. Rapid detection of inducers of enzyme that protect against carcinogen. Proc Natl Acad Sci USA 89: 2394-2398   DOI   ScienceOn
5 Prochaska HJ. 1994. Screening strategies for the detection of anticarcinogenic enzyme inducers. J Nutr Biochem 5: 360-366   DOI   ScienceOn
6 Hong EY, Kang HJ, Kwan CS, Na YJ, Suh MJ, Kim JS. 1997. Modulation of cellular quinone reductase inducibility by roasting treatment and acid hydrolysis of perilla. J Korean Soc Food Sci Nutr 26: 186-192
7 Prochaska HJ, Santamaria AB. 1988. Direct measurement of NAD(P)H:quinone reductase from cells cultured in microtiter wells: A screening assay for anticarcinogenic enzyme inducers. Anal Biochem 169: 328-336   DOI   ScienceOn
8 Budd GT, Osgood B, Barna B, Boyett IM, Finke J, Mdendrop SV, Murth S, Bukoski RM. 1989. Phase I clinical trial toxicity and immunologic effects. Cancer Res 49: 6432-6439
9 Bae MJ, Yee ST, Chae SY, Shin SH, Kweon SH, Park MH, Song MK, Hwang SJ. 2004. The effect of arabinoxylane and the polysaccharide peptide (PSP) on the antiallergy, anticancer. J Korean Soc Food Sci Nutr 33: 469-474   DOI   ScienceOn
10 Giampietri A. 1981. Drug-mediated increase of tumor immunogenicity in vivo for a new approach to experimental cancer immunotherapy. Cancer Res 41: 681-685
11 Kim JS. 2005. Cancer chemoprevention and NSAID-activated gene (NAG-1). Biochemistry and Molecular Biology News 25: 286-291
12 Cha BC, Lee HW, Choi MY. 1998. Antioxidative and antimicrobial effects of nut species. Kor J Pharmacogn 29: 28-34
13 Park SH, Kim KS. 2004. Isolation and identification of antioxidant flavonoids from Salicornia herbacea. J Korean Soc Appl Biol Chem 47: 120-123
14 Wefers H, Komai T, Talalay P, Sies H. 1984. Protection against reactive oxygen species by NAD(P)H:quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). FEBS Lett 169: 63-66   DOI   ScienceOn
15 Schwartsmann G, Rocha AB, Berlinck RGS, Jimeno J. 2001. Marine organisms as a source of new anticancer agents. Lancet Oncology 2: 221-225   DOI   ScienceOn
16 Park JC, Choi JW. 1996. Screening of marine natural products on inhibitory effect of the formation of lipid peroxidation. Kor J Pharmacogn 27: 117-122
17 Shin KS, Boo HO, Jeon MW, Ko JY. 2002. Chemical components of native plant, Salicornia herbacea L. Korean J Plant Res 15: 216-220
18 Han SK, Kim SM. 2003. Antioxidative effect of Salicornia herbacea L. grown in closed sea beach. J Korean Soc Food Sci Nutr 21: 207-210
19 Son DY, Ermawati N, Cha JY, Liang YS, Jung MH, Shin DJ, Lee BH, Lee KH. 2004. Molecular cloning and characterization of outer envelope membrane protein from Salicornia herbacea. J Plant Biotechnol 31: 273-278   DOI   ScienceOn
20 Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbortt BJ, Mayo JG, Shoemarker RH, Boyd MR. 1988. Feasibility of drug screening with panels of human tumor cell line using a microculture tetrazolium assay. Cancer Res 48: 589-601
21 Carmichael J, De Graff WG, Gazder AF, Minna JD, Mitchell JB. 1987. Evaluation of the tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47: 936-942
22 Lee HJ, Kim YA, Ahn JW, Lee BJ, Moon SG, Seo YW. 2004. Screening of peroxynitrite and DPPH radical scavenging activities from salt marsh plants. Kor J Biotechnol Bioeng 19: 57-61
23 Hwang BH, Zhao JL, Choi KP, Jung SW, Kim EJ, Ham SS. 1996. The antimutagenic and anticancer effect of Taxus cuspidata extracts. J Korean Soc Food Sci Nutr 25: 1062-1068
24 Yoo KY, Shin HR. 2003. Cancer epidemiology and prevention. Kor J Epidemiology 25: 1-15
25 Park SY, Joung YH, Shin MO, Joung BM, Bae SJ. 2005. Effect of antimicrobial and cytotoxicity of Undaria pinnatifida Sporophyll fractions. J Korean Soc Food Sci Nutr 34: 765-770   DOI   ScienceOn