Browse > Article
http://dx.doi.org/10.3746/jkfn.2008.37.10.1363

Extraction and Analysis of Astaxanthin from Haematococcus pluvialis Using Sonication  

Kim, So-Young (Dept. of Food and Biotechnology, Center for Food Function and Safety, Hoseo University)
Cho, Eun-Ah (Dept. of Food and Biotechnology, Center for Food Function and Safety, Hoseo University)
Yoo, Ji-Min (Dept. of Food and Biotechnology, Center for Food Function and Safety, Hoseo University)
In, Man-Jin (Dept. of Human Nutrition and Food Science, Chungwoon University)
Chae, Hee-Jeong (Dept. of Food and Biotechnology, Center for Food Function and Safety, Hoseo University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.37, no.10, 2008 , pp. 1363-1368 More about this Journal
Abstract
The extraction and quantitative analysis conditions for astaxanthin from Haematococcus pluvialis, and the structural characteristics of H. pluvialis extract, H. pluvialis hydrolysate and synthetic astaxanthin were investigated using UV/visible and FT-IR spectrometers. Astaxanthin was dissolved in methanol, and then treated to enhance the solubility by sonication for 45 min. With sonication pretreatment, the solubility of astaxanthin increased up to 1.5 times compared to that without sonication. The extracts were hydrolyzed by cholesterol esterase for the analysis of H. pluvialis extract containing astaxanthin ester. A HPLC method using reverse phase C18 column with methanol-water (95:5, v/v) as mobile phase was developed to analyze astaxanthin. After hydrolysis, the absorption spectrum of H. pluvialis hydrolysat was changed to similar pattern to synthetic astaxanthin, confirming the extraction and analysis condition of astaxanthin from H. pluvialis.
Keywords
astaxanthin; Haematococcus pluvialis; HPLC analysis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Kim ZH, Kim SH, Lee HS, Lee CG. 2006. Enhanced production of astaxanthin by flashing light using Haematococcus pluvialis. Enzyme Microb Technol 39: 414-419   DOI   ScienceOn
2 Han JY, Lee SJ, Jung MK, Choi SK. 2000. Process for extracting astaxanthin pigment from blue-green algae and extracted pigment thereof. Korean Patent 0411364
3 Park EK, Seo MW, Lee CK. 2001. Effects of medium compositions for the growth and the astaxanthin production of Haematococcus pluvialis. Kor J Appl Microbiol Biotechnol 29: 227-233
4 Chen X, Chen R, Guo Z, Li C, Li P. 2007. The preparation and stability of the inclusion complex of astaxanthin with $\beta$-cyclodextrin. Food Chem 101: 1-5   DOI   ScienceOn
5 Renstrom B, Borch G, Skulberg OM, Jensen SL. 1981. Optical purity of (3S,3'S)-astaxanthin from Haematococcus pluvialis. Phytochemistry 20: 2561-2564   DOI   ScienceOn
6 Yuan C, Jin Z, Xu X, Zhuang H, Shen W. 2008. Preparation and stability of the inclusion complex of astaxanthin with hydroxypropyl-$\beta$-cyclodextrin. Food Chem 109: 264-268   DOI   ScienceOn
7 Bendich A. 1991. Non vitamin a activity of carotenoids: immuno enhancement. Food Sci Technol Res 2: 127-130   DOI   ScienceOn
8 Kobayashi M. 2003. Astaxanthin biosynthesis enhanced by reactive oxygen species in the green alga Haematococcus pluvialis. Biotechnol Bioprocess Eng 8: 322-330   과학기술학회마을   DOI   ScienceOn
9 Lim KB, Lee EK, Lee SY. 2003. Extraction method of astaxanthin using supercritical fluid extraction. Korean Patent 0465556
10 Margalith PZ. 1999. Production of ketocarotenoids by microalgae Appl Microbiol Biotechnol 51: 431-438   DOI
11 Chew BP, Park JS, Wong MW, Wong TS. 1999. A comparison of the anticancer activities of dietary beta-carotene, canthaxanthin and astaxanthin in mice in vivo. Anticancer Res 19: 1849-1854
12 Jyonouchi H, Sun S, Iijima K, Gross M. 2000. Antitumor activity of astaxanthin and its mode of action. Nutr Cancer 36: 59-65   DOI   ScienceOn
13 Snodderly DM. 1995. Evidence for portection against age-related macular degeneration by carotenoids andantioxidant vitamins. Am J Clin Nutr 62: 1448-1461   DOI
14 Xu BJ, Fang ZM, Kang HJ, Wang QJ, Mo EK, Sung CK. 2003. Liquid chromatographic determination of astaxanthin in Phaffia rhodozyma. Kor J Food Nutr 16: 165-170   과학기술학회마을
15 Shimidzu N, Goto M, Miki W. 1996. Carotenoids as singlet oxygen quenchers in marine organisms. Fish Sci 62: 134-137   DOI
16 Grung M, Jensen SL. 1991. Algal carotenoids 51. Secondary carotenoids 2. Haematococcus pluvialis aplanospores as a source of (3S,3S)-astaxanthin esters. J Appl Phycol 4: 165-171   DOI
17 Kang CD, Park TH, Sim SJ. 2006. Biological $CO_2$ fixation to antioxidant carotenoids by photosynthesis using the green microalga Haematococcus pluvialis. Kor Chem Eng Res 44: 46-51
18 Orosa M, Franqueira D, Cid A, Abalde J. 2005. Analysis and enhancement of astaxanthin accumulation in Haematococcus pluvialis. Bioresour Technol 96: 373-378   DOI   ScienceOn
19 Yun MJ, Kim JH, Choi HY, Kim SK, Gu YS. 2004. The manufacturing method of high-enriched astaxanthin. Korean Patent 0543088
20 Kraay GW, Zapata M, Veldhuis MJ. 1992. Separation of chlorophylls c-1, c-2, and c-3 of marine phytoplankton by reversed phase C18 high performance liquid chromatography. J Phycol 28: 708-712   DOI
21 Lee S, Lee YK, Soh CW. 1991. Accumulation of astaxanthin in Haematococcus lacustris (Chlorophyta). J Phycol 27: 575-577   DOI
22 Schroeder WA, Johnson EA. 1993. Antioxidant role of carotenoids in Phaffia rhodozyma. J Gen Microbiol 39: 907-912
23 Olaizola M. 2000. Commercial production of astaxanthin from Haematococcus pluvialis using 25000-liter outdoor photobioreactors. J Appl Phycol 12: 499-506   DOI   ScienceOn
24 Lee CK. 2003. Process development for astaxanthin production by microalgae. Ministry of Maritime Affairs & Ficheries, Korea
25 Johnson EA, Schroeder WA. 1995. Microbial carotenoids production. Adv Biochem Eng 53: 119-178
26 Sedmak JJ, Weerasinghe DK, Jolly SO. 1990. Extraction and quantitation of astaxanthin from Phaffia rhodozyma. Biotechnol Technol 4: 107-112   DOI
27 Lorenz RT, Cysewski GR. 2000. Commercial potential for Haematococcus microalgae as a natural source of astaxanthin. Tibtech 18: 160-167   DOI   ScienceOn