Physiological Function and Development of Beverage from Grifola frondosa

Contents of polyphenol compounds and the physiological activity of extracts from Grifola frondosa by water and methanol extraction were investigated to determine their functional effects. A functional beverage was developed using the extracts. The yield and phenolic compounds content of the water extracts were highest (49.2% and 327 mg/100 g, respectively), while for the methanol extraction method they were 28.7% and 130 mg/100 g, respectively. DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity was 76.3% for the water extract and 65.4% for methanol extract, whereas the superoxide dismutase (SOD)-like activity was low ($26.3{\sim}36.8%\;at\;1,000{\mu}g/mL$ concentration) Angiotensin converting enzyme (ACE) inhibitory effect of water extract (75.1%) was higher compared to the methanol extracts (41.2%). Tyrosinase inhibition activity was 42.5% for the water extracts and 31.8% for the methanol extracts at $1,000{\mu}g/mL$ concentration. The most acceptable formulation for G. frondosa beverage developed was 0.5% G. frondosa water extract, 8.0% oligosaccharide, 2.0% green tea extract, 2.0% jujube extract, 1.0% Solomon's seal extract, 0.01% vitamin C, and 2.0% apple extract. The final product had 9.8 Brix and color values of L, 35.2+1.1; a, 3.2+0.2; b, 13.6+0.3.

잎새버섯 추출물의 생리 기능 및 음료 제조

잎새버섯을 건조시 킨 후 메탄올과 물로 추출하여 생리활성을 측정하고 관능검사로 최적 음료 조건을 조사하였다. 건조한 잎새버섯을 메탄올과 물로 추출한 후 수율과 폴리페놀 함량은 메탄을 추출물 28.7%, 130 mg/100 g, 물 추출물 49.2%, 327 mg/100 g으로 물 추출물이 수율 1.7배, 폴리페놀 함량은 2.5배가량 높았다. 잎새버섯의 DPPH 라디칼 소거능은 메탄을 추출물에서 65.4%,물 추출물에서 76.3%으로 높았으나 SOD 유사활성능은 $1,000{\mu}g/mL$에서 $26.3{\sim}33.8%$로 낮은 것으로 나타났다. 또한, ACE 저해 효과는 메탄을 추출물에서 41.2%,물 추출물에서 75.1%로 나타났다. Tyrosinase 억제 효과는 메탄을 추출물에서 31.8%, 물 추출물에서 42.5%이었다. 이상과 같이 메탄을 추출물보다는 물 추출물에서 생리적 기능이 높음을 확인하였기에 물 추출물을 음료 제조에 이용하였다. 그 결과, 잎새버섯 음료의 최적 배합비는 잎새버섯 물 추출물 0.5%, oligosaccharide 8.0%, green tea extract 2%, jujube extract 2%, solomon's seal extract 1.0%, vitamin C 0.01%, apple extract 2%이었으며, 이 때 최종 음료의 당도는 9.8 Brix, 색도는 L, $35.05{\pm}1.08;$ a, $3.24{\pm}0.19;$ b, $13.64{\pm}0.33$으로 나타났다.