Browse > Article
http://dx.doi.org/10.3746/jkfn.2006.35.9.1232

Anti Complementary Polysaccharides in Grape Wines  

Park, So-Yeon (Dept. of Food Science and Biotechnology, Kyonggi University)
Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University)
Yu, Kwang-Won (Division of Food and Biotechnology, Chungju National University)
Han, Nam-Soo (Dept. of Food Science and Technology, Chungbuk National University)
Lee, Ho (Dept. of Food Science and Biotechnology, Kyonggi University)
Koh, Jong-Ho (Insung Pharm Co., Ltd.)
Shin, Kwang-Soon (Dept. of Food Science and Biotechnology, Kyonggi University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.35, no.9, 2006 , pp. 1232-1236 More about this Journal
Abstract
Crude polysaccharide fractions of commercially available grape wines (red wine, white wine and wild grape wine) were prepared by evaporation and ethanol precipitation to confirm and identify anti complementary polysaccharides in the wines. When these fractions were evaluated for their anti complementary activity, crude polysaccharide fractions of red wine (RW-0) and wild grape wine (WGW-0) showed higher anti-complementary activities than those of white wine (WW-0). RW-0 and WW-0 were further fractionated into RW-1, WW-1 as high-molecular fractions, and RW-2, WW-2 as low-molecular fractions through gel permeation column chromatography on Sephadex G-75. RW-1 had the most potent activity with the highest carbohydrate content (91.3%). Anti-complementary activity of red wine was higher than that of white wine, suggesting that active polysaccharides such as pectin and hemicellulose are mainly distributed in the grape skin which is removed during white wine making. In addition, high molecular fractions, RW-1 and WW-1 with high contents of carbohydrate and high yields showed higher activities than those of low molecular fractions, RW-2 and WW-2.
Keywords
grape wine; anti-complementary activity; polysaccharide; gel permeation chromatography;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Jakobisiak M, Lasek W, Golab J. 2003. Natural mechanisms protecting against cancer. Immunol Lett 90: 103-122   DOI   ScienceOn
2 Frankel EN, Kanner J, German JB, Parks E, Kinsella JE. 1993. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. Lancet 20: 454-457
3 Blumenkrantz N, Asboe-Hansen G. 1973. New method for quantitative determination of uronic acid. Anal Biochem 54: 484-489   DOI   ScienceOn
4 Lowry OH, Rosebrough NJ, Farr AL, Randoll RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-274
5 Karkhanis YD, Zeltner JY, Jackson JJ, Carlo DJ. 1978. A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of Gram-negative bacteria. Anal Biochem 85: 595-601   DOI   ScienceOn
6 Dragon-Durey MA, Fremeaux-Bacchi V. 2006. Complement component deficiencies in human disease. Presse Med 35: 861-870   DOI
7 Rus H, Cudrici C, Nuculescu F. 2005. The role of the complement system in innate immunity. Immunol Res 33: 103-112   DOI   ScienceOn
8 Ptak J, Lochman J. 2005. Immunoadsorption therapy and complement activation. Transfus Apher Sci 32: 263-267   DOI   ScienceOn
9 Janeway CA. 1968. Progress in immunology. Syndromes of diminished resistance to infection. J Pediatr 72: 885-903   DOI
10 Yamaguchi N, Sakai T, Yoshida S, Katayama Y, Kawai K. 1983. Anti-tumor activity of endotoxin depends on activation of serum complement fragments. Gastroenterol Jpn 18: 436-439   DOI
11 Kim ZY. 1986. Activations of the complement system. Allergy 6: 61-66   DOI
12 Mayer MM. 1971. Complement and complement fixation. In Experimental Immunolochemistry. 2nd ed. Kabat EA, Mayer MM, eds. Charles C Thomas Publisher, Illinois. p 133-240
13 Albersheim P, An J, Freshour G, Fuller MS, Guillen R, Ham KS, Hahn MG, Huang J, O'Neill M, Whitcombe A, Williams MV, York WS, Darvill AG. 1994. Structure and function studies of plant cell wall polysaccharide. Biochem Soc Trans 22: 374-378   DOI
14 Tsukagoshi S, Hashimoto Y, Fujii G, Kobayashi H, Nomoto K, Orita K. 1984. Krestin (PSK). Cancer Treat Rev 11: 131-155   DOI   ScienceOn
15 Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350-356   DOI
16 Sinclair D, Wilde G, Bex S, Peters S. 2005. Complementing the patient: a complement component deficiency in a patient with recurrent infections and glomerulonephritis. Clin Lab 51: 505-507
17 Korean Alcohol Liquor Industry Association. 2001. Alcoholic Beverage News. Seoul. p 11-15