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http://dx.doi.org/10.3746/jkfn.2006.35.9.1166

Effects of Silk Protein Hydrolysates on Blood Glucose in C57BL/KsJ db/db Mice  

Shin, Mi-Jin (Dept. of Food Science and Nutrition, Dankook University)
Park, Min-Jeong (Dept. of Food Science and Nutrition, Dankook University)
Youn, Myung-Sub (Dept. of Food Science and Nutrition, Dankook University)
Lee, Young-Sook (Dept. of Food Science and Nutrition, Dankook University)
Nam, Moon-Suk (Dept. of Internal Medicine, Inha University, College of Medicine)
Park, In-Sun (Dept. of Anatomy, Inha University, College of Medicine)
Jeong, Yoon-Hwa (Dept. of Food Science and Nutrition, Dankook University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.35, no.9, 2006 , pp. 1166-1171 More about this Journal
Abstract
This study was performed to investigate the effect of silk protein hydrolysates on blood glucose in diabetic mice (C57BL/KsJ db/db). The silk protein hydrolysates hydrolyzed by protease contains 87.52% of peptides of which molecular weight was below 2,000 dalton. The content of free amino acids was 14.80 g/100 g silk protein hydrolysates and major free amino acids were Pro, Thr, Arg and Ala. Silk protein hydrolysates were administered to the animals for 9 weeks at doses of 0.2, 0.5% and 0.8% solution. The body weight increase in the 0.5 and 0.8% fed groups were higher than control group. Food and water intake in the silk protein hydrolysates fed groups were lower than control group. The weight of liver was not different among groups, while the weight of kidney in control group was higher than silk protein hydrolysates fed groups. The blood glucose level in silk protein hydrolysates fed groups was lower than control group. In the glucose tolerance test, the blood glucose level in control group was the highest at 15 minutes after glucose injection while those in silk protein hydrolysates fed groups were the highest at 30 minutes. Results in this study suggest that silk protein hydrolysates show hypoglycemic effect in C57BL/KsJ db/db mice.
Keywords
diabetic mice; silk protein hydrolysates; blood glucose;
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1 Coulston AM, Hollenbeck CB. 1988. Source and amount of dietary carbohydrate in patients with non insulin-dependent diabetes mellitus. Top Clin Nutr 3: 17-24
2 DeFronzo RA. 1988. Lilly Lecture: the triumvirate: $\beta$-cell, muscle, liver: a collusion responsible for NIDDM. Diabetes 37: 667-687   DOI
3 DeFronzo RA, Jacot E, Jequier E, Maeder E, Wahren J, Felber JP. 1981. The effect of insulin on the disposal of intravenous glucose: results from indirect calorimetry. Diabetes 30: 1000-1007   DOI
4 DeFronzo RA. 1992. Pathogenesis of type 2 (non-insulin dependent) diabetes mellitus: a balanced overview. Diabetalogia 35: 389-397   DOI
5 Bailey CJ. 1999. Insulin resistance and antidiabetic drugs. Biochem Pharmacol 58: 1511-1520   DOI   ScienceOn
6 Shokuhin to kaihatsu Henshubu. 2000. Market trend of health foods and health ingredients. Shokuhin to Kaihatsu 35: 18-34
7 Lee KG, Yeo JH, Lee YW, Kweon HY, Woo SO, Han SM, Kim JH. 2003. Studies on industrial utilization of silk protein. Kor J Food Sci Ind 36: 25-37
8 Lee SH, Cho HN, Hyun CK, Jew SS. 2002. Physiology functional characteristic of silk peptide. Food Sci Ind 35: 57-62
9 Luo J, Chen K, Xu Q, Hirabayashi K. 1993. Study on foodization of fibroin and its functionality. The collection of paper for the second international silk conference, Beijing, China. p 73-87
10 Gotoh K, Izumi H, Kanamoto T, Tamada Y, Nakashima H. 2000. Sulfate fibroin, a novel sulfated peptide derived from silk, inhibits human immunodeficiency virus replication in vitro. Biosci Biotechnol Biochem 64: 1664-1670   DOI   ScienceOn
11 Stanley M, Lee SB. 1986. Chronic effects of an $\alpha$-glucosidase inhibitor (Bay O 1248) on intestinal disaccharidase activity in normal and diabetic mice. J Pharm Exp Therapeutics 240: 123-137
12 Hong H, Maeng WJ. 2004. Effect of malted barley extract and banana extract on blood glucose levels in genetically diabetic mice. J Med Food 7: 487-490   DOI   ScienceOn
13 Jung UJ, Lee MK, Jeong KS, Choi MS. 2004. The hypoglycemic effect of hesperidin and naringin are partely mediated by glucose-regulating enzymes in C57BL/KsJ db/db mice. J Nutr 134: 2499-2503
14 Uchiyama K, Naito Y, Hasegawa G, Nakamura N, Takahashi J, Yoshikawa T. 2002. Astaxanthin protects beta-cells against glucose toxicity diabetic db/db mice. Redox Rep 7: 290-293   DOI   ScienceOn
15 Kim MJ, Ahn JH, Choi KH, Lee YH, Woo GJ, Hong EK, Chung YS. 2006. Effect of pine needle extract oil on blood glucose and serum insulin levels in db/db mice. J Korean Soc Food Sci Nutr 35: 321-327   과학기술학회마을   DOI   ScienceOn
16 Akai H. 1999. New physiological functions of silk material. Shokuhin to Kaihatsu 34: 43-47
17 DeFronzo RA, Gunnarsson R, Bjorkman O, Olsson M, Wahren J. 1985. Effects of insulin on peripheral and splanchnic glucose metabolism in non-insulin dependent diabetes mellitus. J Clin Invest 76: 149-155   DOI
18 Grey NJ, Karls I, Kipinis DM. 1975. Physiological mechanism in the development of starvation ketosis in man. Diabetes 24: 10-14   DOI
19 Ishihara K, Fukuchi Y, Mizunoya W, Mita Y, Fukuya Y, Fuhiki T, Yasumoto K. 2003. Amino acid composition of soybean protein increased postprandial carbohydrate oxidation in diabetic mice. Biosci Biotechnol Biochem 67: 2505-2511   DOI   ScienceOn
20 Park KJ, Hong SE, Do MS, Hyun CK. 2002. Stimulation of insulin secretion by silk fibroin hydrolysate in streptozotocin-induced diabetic rat and db/db mice. Kor J Pharmacogn 33: 21-28   과학기술학회마을
21 Zeman FJ. 1991. Clinical nutrition and diabetics. 2nd ed. Macmillan Publishing Company, New York. p 398-403
22 Nahm JH, Oh YS. 1995. A study of pharmacological effect of silk fibroin. RDA J Agric Sci 37: 145-157
23 Gallaher DD, Casallany AS, Shoeman DW, Olson JM. 1993. Diabetes increases excretion of urinary malonaldehyde conjugates in rat. Lipid 28: 663-666   DOI
24 Zhang BB, Moller DE. 2000. New approaches in the treatment of type 2 diabetes. Curr Opin Chem Biol 4: 461-467   DOI   ScienceOn
25 DeFronzo RA, Ferrannini E. 1987. Regulation of hepatic glucose metabolism in humans. Diabetes Metab Rev 3: 415-459   DOI   ScienceOn