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http://dx.doi.org/10.3746/jkfn.2006.35.6.671

Effect of [6]-Gingerol on Bcl-2 and Bax Expression in MDA-MB-231 Human Breast Cancer Cell Line  

Seo, Eun-Young (Dept. of Food Science and Nutrition, Dankook Uniuersity)
Kim, Woo-Kyung (Dept. of Food Science and Nutrition, Dankook Uniuersity)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.35, no.6, 2006 , pp. 671-676 More about this Journal
Abstract
We investigated the effect of gingerol (Zingiber officinale Roscoe, Zingiberaceae) on Bcl-2 and Bax expression in MDA-MB-231 human breast cell lines. The oleoresin from rhizomes of ginger contains [6]-gingerol (1-[4'-hydroxy-3'-methoxyphenyl]-5-hydroxy-3-decanone). We previously reported that [6]-gingerol inhibits cell proliferation in MDA-MB-231 human breast cancer cell lines. In this study, we examined protein and mRNA expression associated with cell apoptosis in MDA-MB-231 human breast cancer cell lines. We cultured MDA-MB-231 cells in presence of various concentrations 0, 2.5, 5 and $10\;{\mu}M$ of [6]-gingerol. Bcl-2 protein and its mRNA levels were decreased dose-dependently in cells treated with [6]-gingerol, but Bax protein and its mRNA levels were unchanged by [6]-gingerol treatment. Bcl-2/Bax ratio was decreased in a dose dependent manner treated with [6]-gingerol. Caspase-3 activity was significantly increased dose-dependently in cell treated with [6]-gingerol (p<0.05). In conclusion, we have shown that [6]-gingerol induces apoptosis in MDA-MB-231 human breast cancer cell lines.
Keywords
[6]-gingerol; breast cancer cell; apoptosis; Bax; Bcl-2; caspase 3;
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