Browse > Article
http://dx.doi.org/10.3746/jkfn.2006.35.5.536

Beneficial Effects of Water Extracts of Scutellariae Radix on Immune Function in Mice Fed Alcohol  

Lee, In-Seok (Dept. of Medical Nutrition, Graduate School of Ease-West, Medical Science, Kyung Hee University)
Kang, Kyoung-Lan (Research Institute of Clinical Nutrition, Kyung Hee University)
Choue, Ryo-Won (Dept. of Medical Nutrition, Graduate School of Ease-West, Medical Science, Kyung Hee University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.35, no.5, 2006 , pp. 536-542 More about this Journal
Abstract
High fat diet and chronic alcohol consumption cause hyperlipidemia, steatohepatitis and in some cases, cirrhosis. But the detailed mechanisms are not known. Scutellariae Radix (SR) has been known to have hepatoprotective effects. The aim of this study was, therefore, to determine whether SR water extract (100 mg/kg) could affect immune function in mice abused by long-term alcohol consumption (feed 25% ethanol in water for 1 month, ad libitum) with high fat diet (40% fat of total calorie). Mice received either a regular diet (RD, AIN 93) or a high fat diet (HD); high fat diet group were divided into ethanol group (HED) or ethanol with SR water extract group (HEDS). Food consumption was measured daily and body weights recorded weekly throughout the experiment. Immunological parameters (Ig A, Ig E, TNF-$\alpha$, IFN-$\gamma$, IL-$1{\beta}$) were measured from the serum and the supernatant of spleen lymphocytes from the all groups. The concentration of serum Ig A, Ig E and cytokines were significantly higher in the alcohol consumed groups. Also the concentration of supernatant of spleen lymphocytes, Ig A, Ig E, cytokines were significantly higher in the ethanol consumption groups. Otherwise, HEDS group were significantly lower than HED group. These results suggest that SR water extract may improve the haptic immune function in mice fed high fat diet with alcohol.
Keywords
Scutellariae Radix; alcohol; high fat diet; immunoglobulin; cytokine;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Latvala J, Hietala J, Koivisto H, Jarvi K, Anttila P, Niemela O. 2005. Immune responses to ethanol metabolites and cytokine profiles differentiate alcoholics with or without liver disease. Am J Gastroenterol 100: 1303-1310   DOI   ScienceOn
2 Kevin W, Graeme A. 2000. Alcoholic liver disease. Postgrad Med J 76: 280-286   DOI
3 Bai J, Odin JA. 2003. Apoptosis and the liver: relation to autoimmunity and related conditions. Autoimmunity Rev 2: 36-42   DOI   ScienceOn
4 Park JW, Yoo BC. 2001. Pathogenesis of nonalcoholic steatohepatitis: Role of lipid peroxidation mitochondrial dysfunction and cytokines. Kor J Gastroenterol 38: 1-8
5 Taylor AN, Tio DL, Yirmiya R. 1999. Fetal alcohol exposure attenuates interleukin-1$\beta$-induced fever: neuroimmune mechanisms. J Neuro 99: 44-52
6 McClain CJ, Barve S, Deaciuc I, Kugelmas M, Hill D. 1999 Cytokines in alcoholic liver disease. Semin Liver Dis 19: 205-219   DOI   ScienceOn
7 Hoek JB, Pastorino JG. 2002. Ethanol, oxidative stress, and cytokine-induced liver cell injury. Alcohol 27: 63-68   DOI   ScienceOn
8 Lim BO, Yamada K, Yoshimura K, Watanabe T, Pham H, Taniguchi S, Sugano M. 1995. Free bile acids inhibit Ig E production by mouse spleen lymphocytes stimulated by lipopolysaccharide and interleukins. Biosci Biotech Biochem 59: 624-627   DOI   ScienceOn
9 Lim BO, Jolly CA, Zaman K, Fernandes G. 2000. Dietary (n-6) and (n-3) fatty acids and energy restriction modulate mesenteric lymph node lymphocyte function in autoimmune-prone (NZBxNZW)F1 mice. J Nutr 130: 1657-1664
10 Robinson RS, Seelig LL Jr. 2002. Effects of maternal ethanol consumption on hematopoietic cells in the rat fetal liver. Alcohol 28: 151-156   DOI   ScienceOn
11 Perlemuter G, Letteron P, Carnot F, Zavala F, Pessayre D, Nalpas B, Brechot C. 2003. Alcohol and hepatitis C virus core protein additively increase lipid peroxidation and synergistically trigger hepatic cytokine expression in a transgenic mouse model. J Hepatol 39: 1020-1027   DOI   ScienceOn
12 Viitala K, Israel Y, Blake JE, Niemela O. 1997. Serum Ig A, Ig G, and Ig M antibodies directed aganist acetaldehyde- derived epitopes: relationship to liver disease severity and alcohol consumption. Hepatololgy 25: 1418-1424   DOI   ScienceOn
13 Lee HJ. 1994. Alcohol and Dyslipidemia. Kor J Lipidol 4: 119-122
14 Ha TY, Park YM, Chun SN, Lee JH, Lee HK, Kim JS. 1990. Effect of alcohol on immune response in mice. Korea Soc Microbiol 23: 265-281
15 McClain CJ, Song Z, Barve SS, Hill DB, Deaciuc I. 2004. Recent advance in alcoholic liver disease IV. dysregulated cytokine metabolism in alcohol liver disease. Am J Physiol Gastrointest Liver Physiol 287: 497-502   DOI   ScienceOn
16 Abe K, Inoue O, Yumioka E. 1990. Biliary excretion of metabolites of baicalin and baicalein in rats. Chem Pharm Bull 38: 208-211   DOI
17 Faist E. 1996. The mechanism of host defense dysfunction following shock and trauma. Curr Top Microbiol Immunol 216: 259-274
18 Valles SL, Blanco AM, Azorin I, Guasch R, Pascual M, Gomez-Lechon MJ, Renau-Piqueras J, Guerric C. 2003. Chronic ethanol consumption enhances interleukin-1-mediated signal trans-duction in rat liver and in cultured hepatocytes. Alcohol Clin Exper Res 27: 1979-1986   DOI   ScienceOn
19 Moon YH, Lee YH, Min BS, Bae KH. 1997. Antibacterial Constituents from Scutellariae Radix against Streptococcus mutans OMZ176. Kor J Pharmacogen 28: 99-103   과학기술학회마을
20 Koda A. 1982. Anti-allergic actions of traditional oriental medicine--actions against types I and IV hypersensitivity reactions. Nippon Yakurigaku Zasshi 80: 31-41   DOI   ScienceOn
21 Kubo M, Matsuda H, Tanaka M, Kimura Y, Okuda H, Higashino M, Tani T, Namba K, Arichi S. 1984. Studies on Scutellariae radix: VII. Anti-arthritic and anti-inflammatory actions of methanolic extract and flavonoid components from Scutellariae radix. Chem Pharm Bull 32: 2724-2729   DOI   ScienceOn
22 Liaw J, Gau YY, Chao YC. 1999. Effect of baicalein on tracheal permeability in ovalbumin (OA)- sensitized guinea pigs. Pharmaceutical Research 16: 1653-1657   DOI   ScienceOn
23 Friedwald WT, Levy RI, Fedreicson DS. 1972. Estimation of the concentration of low-denisty lipprotein cholesterol in plasma, without was of the preparative ultracentrifuge. Clim Chem 18: 499-506
24 Taieb J, Delarche C, Ethuin F, Selloum S, Poynard T, Gougerot-Pocidalo MA, Chollet-Martin S. 2002. Ethanol- induced inhibition of cytokine release and protein degranulation in human neutrophils J Leukocyte Biology 72: 1142-1147
25 Kim DJ, Kim W, Yoon SJ, Cho BM, Kim JS, Jeong JS. 2003. Effect of alcohol hangover on cytokine production in healthy subjects. Alcohol 31: 167-170   DOI   ScienceOn
26 Dominguez-Santalla MJ, Vidal C, Vinuela J, Perez LF, Gonzalez-Quintela A. 2001. Increased serum IgE in alcoholics: Relationship with Th1/Th2 cytokine production by stimulated blood mononuclear cells. Alcoh Clin Exp Res 25: 1198-1205   DOI   ScienceOn
27 Kim HC. 2001. Textbook of herbal pharmacology. Jipmundang. p 129-133
28 Nanji AA, Zhao S, Sadrzadeh SM, Waxman DJ. 1994. Use of reverse transcription-polymerase chain reaction to evaluate in vivo cytokine gene expression in rats fed ethanol for long periods. Hepatology 19: 1483-1487
29 Colell A, Garcia-Ruiz C, Miranda M, Ardite E, Mari M, Morales A, Corrales F, Kaplowitz N, Fernandez-Checa J. 1998. Selective glutathione depletion of mitochondria by ethanol sensitizes hepatocytes to tumor necrosis factor. Gastroenterology 115: 1541-1551   DOI   ScienceOn
30 McClain CJ, Barve S, Deaciuc I, Kugelmas M, Hill D. 1999. Cytokines in alcoholic liver disease. Semin Liver Dis 19: 205-219   DOI   ScienceOn
31 McClain CJ, Barve S, Deaciuc I, Kugelmas M, Hill DB. 1998. Tumor necrosis factor and alcoholic liver disease. Alcohol Clin Exp Res 22(5 Suppl): 248S-252S   DOI
32 Alexander JW. 1990. Mechanism of immunologic suppression in burn injury. J Trauma 30: 70-75   DOI
33 Dinarello CA. 2000. Proinflammatory cytokines. Chest 118: 503-508   DOI   ScienceOn
34 Akerman PA, Cote PM, Yang SQ, McClain CJ, Nelson S, Bagby G, Diehl AM. 1993. Long-term ethanol consumption alters the hepatic response to the regenerative effects of tumor necrosis factor-${\alpha}$. Hepatology 17: 1066-1073   DOI   ScienceOn
35 Tiegs G, Hentschel J, Wendel A. 1992. A T cell-dependent experimental liver injury in mice inducible by concanavalin A. J Clin Invest 90: 196-203   DOI
36 Cabre E, Rodriguez-Iglesias P, Caballeria J, Quer JC, Sanchez-Lombrana JL, Pares A, Papo M, Planas R, Gassull MA. 2000. The spanish group for the study of alcoholic hepatitis. Short- and longterm outcome of severe alcohol- induced hepatitis treated with steroids or enteral nutrition: a multicenter randomized trial. Hepatology 32: 36-42   DOI   ScienceOn
37 Arteel G, Marsano L, Mendez C, Bentley F, McClain CJ. 2003. Advances in alcoholic liver disease. Best Pract Res Clin Gastroenterol 17: 625-647   DOI   ScienceOn
38 Zhou Z, Wang L, Song Z, Lambert JC, McClain CJ, Kang YJ. 2003. A citical involvement of oxidative stress in acute alcohol-induced hepatic TNF-production. Am J Pathol 163: 1137-1146   DOI   ScienceOn