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http://dx.doi.org/10.3746/jkfn.2006.35.5.516

A Study on the Mechanisms by Which the Aqueous Extract of Inonotus obliquus Induces Apoptosis and Inhibits Proliferation in HT-29 Human Colon Cancer Cells  

Kim, Eun-Ji (Silver Biotechnology Research Center, Hallym University)
Lee, Yong-Jin (Chuncheon Bioindustry Foundation)
Shim, Hyun-Kyung (Silver Biotechnology Research Center, Hallym University)
YoonPark, Jung-Han (Silver Biotechnology Research Center, Hallym University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.35, no.5, 2006 , pp. 516-523 More about this Journal
Abstract
The mushroom Inonotus obliquue (IO) has been traditionally used for the treatment of gastrointestinal cancer in Russia, Poland, and most of Baltic countries. To explore the possibility that IO has chemoprevention effects, we examined whether or not the aqueous extract of IO inhibits HT-29 cell growth and investigated tile mechanism for this effect. Cells were incubated in the presence of increasing concentrations of the aqueous extract of IO. The extract substantially inhibited the viable HT-29 cell number in a dose-dependent manner and inhibited 5-bromo-2'-deoxyuridine incorporation into DNA of HT-29 cells. Annexin-V staining followed by flow cytometry revealed that the extract induced apoptosis of HT-29 cells in a dose-dependent manner. Western blot analysis of total cell lysates revealed that the extract induced cleavage of caspase-8, -9 and -3 and poly (ADP-ribose) polymerase, but did not affect the protein levels of Bax and Bcl-2. In addition, the extract dose-dependently increased the activity of caspase-8, -9 and -3. We have demonstrated that the aqueous extract of IO inhibits cell proliferation and induces apoptosis in HT-29 cells, which may be mediated by its ability to activate the caspase pathway.
Keywords
Inonotus obliquue; apoptosis; caspase; colon cancer;
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Times Cited By KSCI : 2  (Citation Analysis)
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