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http://dx.doi.org/10.3746/jkfn.2006.35.3.278

Antioxidant and Anticancer Activities of Extracts from Styela clava According to the Processing Methods and Solvents  

Kim, Jin-Ju (Division of Food Science and Biotechnology, Kyungnam University)
Kim, Sun-Jung (Division of Food Science and Biotechnology, Kyungnam University)
Kim, Sun-Hee (Division of Food Science and Biotechnology, Kyungnam University)
Park, Hae-Ryong (Division of Food Science and Biotechnology, Kyungnam University)
Lee, Seung-Cheol (Division of Food Science and Biotechnology, Kyungnam University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.35, no.3, 2006 , pp. 278-283 More about this Journal
Abstract
Styela clava was processed by four different kinds of method including FR (fresh S. clava), H1 (heat treated S. clava at $110^{\circ}C$ for 15 min) H2 (heat treated S. clava at $120^{\circ}C$ for 5 min), and FD (freeze dried S. clava). Each S. clava sample was treated with methanol, ethanol, acetone, and water, then antioxidant and anticancer activities of the extracts were evaluated. In extracts from non-dried S. clava (FR, H1, and H2), total extract yield decreased with increasing treated temperature. The extraction yield was in the order of ethanol>methanol>water>acetone among treated solvents. In case of dried S. clava (FR), the extraction yield was lower than non-dried samples, and was in the order of methanol>ethanol>water>acetone. The radical scavenging activity (RSA) of non-dried S. clava (FR, H1, and H2) was in the order of acetone>ethanol>methanol and heat treatment also decreased RSA. RSA of FD was the highest in ethanol extract, while acetone and water extracts did not show RSA. When antioxidant activity was determined by reducing power (RD), methanol extract of FR showed the highest values and heat treatment decreased RD, too. RD of FD was in the order of methanol>ethanol>water>acetone. The acetone extracts from FD showed significant anticancer activity against human colon cancer cell line HT-29. These results indicated that extraction yield and properties of extracts from S. clava were dependent on processing temperature, solvent and/or physicochemical state. The appropriate extraction process should provide some valuable bioactive materials from S. clava.
Keywords
Styela clava; antioxidant activity; anticancer; freeze-drying; heat-treatment;
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Times Cited By KSCI : 2  (Citation Analysis)
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