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http://dx.doi.org/10.3746/jkfn.2006.35.1.078

Purification and Chemical Characterisation of Laminaran from Eisenia bicyclis in Korea  

Kim, Young-Myoung (Korea Food Research Institute)
Choi, Yong-Seok (Korea Food Research Institute)
Park, Jong-Hyuk (Korea Food Research Institute)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.35, no.1, 2006 , pp. 78-86 More about this Journal
Abstract
Laminarans with different purity were prepared from Eisenia bicyclis and their structures were characterized. Crude laminaran was successively extracted two times at room temperature for 2 hr with 0.09 N HCl, and partially purified laminaran was isolated using cetylpyridinium chloride (CPC). Crude laminaran accounted for $14.5\%$ of the dry seaweed weight and contained $8.4\%$ protein, $7.6\%$ sulfate and $68.2\%$ polysaccharide. Partially purified laminaran accounted for $6.5\%$ of the dry algal weight and composed of $3.8\%$ protein, $3.2\%$ sulfate and $74.7\%$ total sugar, which is mainly composed of glucose $(83.3\%)$, indicating that partially purified laminaran was more purified polysaccharide than crude laminaran. Purified laminaran was fractionated into one fractions by Sephacryl S-300 HR column chromatography and this fraction was analysed by FT-IR, $^{13}C$ NMR, methylation and gel filtration chromatography. Purified laminaran showed $\beta-configuration$ $(^{13}C:103.0ppm,\;FT-IR:888cm^{-1})$ in the anomerization of the glycosidic linkages and was $(1\rightarrow3),\;(1\rightarrow6)$ linked $\beta-glucan$. The average molecular weight of purified laminaran was 12,600 dalton.
Keywords
Eisenia bicyclis; laminaran; purification; structure;
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