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http://dx.doi.org/10.3746/jkfn.2006.35.1.028

Inhibition of Inflammatory-cytokines Production and Prostaglandin E2 Activity by Puerariae Radix Extracts  

Kim, Si-Na (Korea Medical Science Institute)
Kim, Hee-Seok (Korea Medical Science Institute)
Nam, Gyeong-Sug (Korea Medical Science Institute)
Hwang, Sung-Wan (Korea Medical Science Institute)
Hwang, Sung-Yeoun (Korea Medical Science Institute)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.35, no.1, 2006 , pp. 28-34 More about this Journal
Abstract
The ethanol extracts of Puerariae Radix inhibited cyclooxygenase-2 (COX-2) activity in bone marrow derived mast cells (BMMC). COX-2 is responsible for the production of large amounts of proinflammatory prostaglandins (PGs) at the inflammatory site. We have investigated the anti-inflammatory effect of ethyl acetate fraction from $70\%$ ethanol extract of Puerariae Radix (EPR), and attempted acetic acid induced writhing to verify the analgesic effect. Inflammation was induced by interleukin-1 (IL-1), tumor necrosis factor-a (TNF-a), $inteferon-\gamma$ $(IFN-\gamma)$ and lipopolysaccharide (LPS). EPR showed strong inhibitory efficacy against cytokine-induced proteoglycan degradation, prostaglandin $E_2\;(PGE_2)$ production, nitric oxide (NO) production, and matrix-metalloproteinases (MMPs) expression in mouse macrophage and rabbit articular chondrocyte. In the writhing test, EPR $(200\~400\;mg/kg)$ exhibited a dose-dependent inhibition of writhing. The results indicate that EPR have anti-inflammatory and analgesic activities, and could be a good herbal medicine candidate for treating of osteoarthritis (OA).
Keywords
Puerariae Radix; anti-inflammation; analgesic; osteoarthritis;
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