Browse > Article
http://dx.doi.org/10.3746/jkfn.2005.34.9.1367

Expression and Role of the System L Amino Acid Transporter in FOB Human Osteoblast Cells  

Kim, Chang-Hyun (Oral Biology Research Institute, College of Dentistry, Chosun University)
Park, Joo-Cheol (Oral Biology Research Institute, College of Dentistry, Chosun University)
Kim, Do Kyung (Oral Biology Research Institute, College of Dentistry, Chosun University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.34, no.9, 2005 , pp. 1367-1374 More about this Journal
Abstract
Amino acid transporters play an important role in supplying nutrition to normal and cancer cells for cell proliferation. Amino acid transport system L is a major nutrient transport system responsible for the $Na^+$-independent transport of neutral amino acids including several essential amino acids. The system L is divided into two major subgroups, the L-tyre amino acid transporter 1 (LAT1) and the L-type amino acid transporter 2 (LAT2). In the present study, we have examined the expression and functional characterization of system L amino acid transporters in FOB human osteoblast cells. RT-PCR and western blot analysis have revealed that the FOB cells expressed LAT1, LAT2 together with their associating protein 4F2hc. The uptakes of $[^{14}C]_L$-leucine by FOB cells are $Na^+$-independent and almost completely inhibited by system L amino acid transporter selective inhibitor, 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH). These results suggest that the transport of neutral amino acids including several essential amino acids for cellular nutrition into the FOB human osteoblast cells is mediated by system L amino acid transporters.
Keywords
nutrition to cells; L-type amino acid transporters; FOB cells; essential amino acids; BCH;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Mannion BA, Kolesnikova TV, Lin SH, Wang S, Thompson NL, Hemler ME. 1998. The light chain of CD98 is identified as E16/TA1 protein. J Biol Chem 273: 33127-33129   DOI   ScienceOn
2 Nakamura E, Sato M, Yang H, Miyagawa F, Harasaki M, Tomita K, Matsuoka S, Noma A, Iwai K, Minato N. 1999. 4F2 (CD98) heavy chain is associated covalently with an amino acid transporter and controls intracellular trafficking and membrane topology of 4F2 heterodimer. J Biol Chem 274: 3009-3016   DOI   ScienceOn
3 Yoon JH, Kim IJ, Kim H, Kim HJ, Jeong MJ, Ahn SG, Kim SA, Lee CH, Choi BK, Kim JK, Jung KY, Lee SU, Kanai Y, Endou H, Kim DK. 2004. Amino acid transport system L is differently expressed in human normal oral keratinocytes and humal oral cancer cells. Cancer Lett 222: 237-245   DOI   ScienceOn
4 Kim DK, Kim IJ, Hwang SA, Kook JH, Lee MC, Shin BA, Bae CS, Yoon SH, Ahn SG, Kim SA, Kanai Y, Endou H, Kim JK. 2004. System L-amino acid transporters are differently expressed in rat astrocyte and C6 glioma cells. Neuroscience Res 50: 437-446   DOI   ScienceOn
5 Wolf DA, Wang S, Panzica MA, Bassily NH, Thompson NL. 1996. Expression of a highly conserved oncofetal gene, TA1/E16, in human colon carcinoma and other primary cancers: homology to Schistosoma mansoni amino acid permease and Caenorhabditis elegans gene products. Cancer Res 56: 5012-5022
6 Gomes P, Soares-da-Silva P. 1999. L-DOPA transport properties in an immortalised cell line of rat capillary cerebral endothelial cells, RBE 4. Brain Res 829: 143- 150   DOI   ScienceOn
7 Yanagida O, Kanai Y, Chairoungdua A, Kim DK, Segawa H, Nii T, Cha SH, Matsuo H, Fukushima J, Fukasawa Y, Tani Y, Taketani Y, Uchino H, Kim JY, Inatomi J, Okayasu I, Miyamoto K, Takeda E, Goya T, Endou H. 2001. Human L -type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines. Biochim Biophys Acta 1514: 291 -302   DOI   ScienceOn
8 Kim DK, Kanai Y, Choi HW, Tangtrongsup S, Chair-oungdua A, Babu E, Tachampa K, Anzai N, Iribe Y, Endou H. 2002. Characterization of the system L amino acid transporter in T24 human bladder carcinoma cells. Biochim Biophys Acta 1565: 112-121   DOI   ScienceOn
9 Kim DK, Yoon JH, Jeon JE, Lee SH. 2004. Expression and functional characterization of neutral amino acid transporter in Hep2 human head and neck squamous cell carcinoma. Int J Oral Biol 29: 51-58
10 Christensen HN. 1990. Role of amino acid transport and countertransport in nutrition and metabolism. Physiol Rev 70: 43-77   DOI
11 Lakshmanan M, Goncalves E, Lessly G, Foti D, Robbins J. 1990. The transport of thyroxine into mouse neurobla-stoma cells, NB41A3: the effect of L-system amino acids. Endocrinology 126: 3245-3250   DOI   ScienceOn
12 Kanai Y, Segawa H, Miyamoto K, Uchino H, Takeda E, Endou H. 1998. Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98). J Biol Chem 273: 23629-23632   DOI   ScienceOn
13 Sang J, Lim YP, Panzica M, Finch P, Thompson NL. 1995. TA1, a highly conserved oncofetal complementary DNA from rat hepatoma, encodes an integral membrane protein associated with liver development, carcinogenesis, and cell activation. Cancer Res 55: 1152- 1159
14 Uchino H, Kanai Y, Kim DK, Wempe MF, Chairoungdua A, Morimoto E, Anders MW, Endou H. 2002. Transport of amino acid-related compounds mediated by L-type amino acid transporter 1 (LAT1): insights into the mechanisms of substrate recognition. Mol Pharmacol 61: 729-737   DOI   ScienceOn
15 Mastroberardino L, Spindler B, Pfeiffer R, Skelly PJ, Loffing J, Shoemaker CB, Verrey F. 1998. Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family. Nature 395: 288-291   DOI   ScienceOn
16 Pfeiffer R, Spindler B, Loffing J, Skelly PJ, Shoemaker CB, Verrey F. 1998. Functional heterodimeric amino acid transporters lacking cysteine residues involved in disulfide bond. FEBS Lett 439: 157-162   DOI   ScienceOn
17 Verrey F, Meier C, Rossier G, Kuhn LC. 2000. Glyco-protein-associated amino acid exchangers: broadening the range of transport specificity. Pflugers Arch 440: 503-512   DOI
18 Pineda M, Fernandez E, Torrents D, Estevez R, Lopez C, Camps M, Lloberas J, Zorzano A, Palacin M. 1999. Identification of a membrane protein, LAT-2, that co-expressed with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small large zwitterionic amino acids. J Biol Chem 274: 19738-19744   DOI
19 Segawa H, Fukasawa Y, Miyamoto K, Takeda E, Endou H, Kanai Y. 1999. Identification and functional characterization of a $Na^{+}$-independent neutral amino acid transporter with broad substrate selectivity. J Biol Chem 274: 19745-19751   DOI
20 Rossier G, Meier C, Bauch C, Summa V, Sordat B, Verrey F, Kuhn LC. 1999. LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine. J Biol Chem 274: 34948-34954   DOI   ScienceOn
21 Utsunomiya-Tate N, Endou H, Kanai Y. 1997. Tissue specific variants of glutamate transporter GLT-1. FEBS Lett 416: 312-316   DOI   ScienceOn
22 Utsunomiya-Tate N, Endou H, Kanai Y. 1996. Cloning and functional characterization of a system ASC-like $Na^{+}$-dependent neutral amino acid tansporter. J Biol Chem 271: 14883-14890   DOI   ScienceOn